Investigation of Sodium Benzoate and Potassium Sorbate in Anti-Diabetic Herbal Drugs
The objective of the study was to assess sodium benzoate and potassium sorbate in some anti-diabetic herbal drugs (ADHDs) collected from local markets using UV-Vis spectrophotometric method. Analytes were measured in bulk and finished drug formulations at 224 nm for Sodium benzoate and 254.5 nm for potassium sorbate. The calibration curve obeyed Beer’s law in the range of 0-40 ppm for sodium benzoate and 0-50 ppm for potassium sorbate and passed the goodness of fit evaluation. Limit of detection, LOD and limit of quantification, LOQ for sodium benzoate was 0.13 pm and 0.40 ppm respectively. The limit of detection, LOD and limit of quantification country is unknown. To conclude, whether the herbal drugs are safe to consume in terms of preservatives, more assessment is required., LOQ for potassium sorbate were 0.28 pm and 0.85 ppm respectively. No samples crossed safety limits for sodium benzoate and potassium sorbate. Overall picture of preservative content in herbal drugs of the country is unknown and, in this study, we identified both preservatives in the drug samples. To conclude, whether the herbal drugs are safe to consume in terms of preservatives, more assessment is required.
Malik, A.K., Blasco, C. & Pico, Y. (2010). Liquid chromatography-mass spectrometry in food safety, Journal of Chromatography A, 1217 (25), 4018–4040. https://doi.org/10.1016/j.chroma.2010.03.015
Akter, S., Zubair, M.A., Khan, M.S.H., Bari, L., Huq, M.A. & Rashid, M.A. (2017). Identification and quantification of sodium benzoate in different brands of mango juices available in Tangail region, Bangladesh, Bangladesh Pharmaceutical Journal, 20(1), 20-26. https://doi.org/10.3329/bpj.v20i1.32089
Sankar, D.G., Babu, A.N., Rajeswari, A. & Krishna, M.V. (2009). RP- HPLC method for estimation of flucloxacillin magnesium and sodium benzoate in oral suspension, Asian Journal of Chemistry, 21 (8), 5843-5846. ISSN: 0970-7077
Hassouna, M.E.M., Abdelrahman, M.M. & Mohamed, M.A. (2017). Validation of a novel and sensitive RP- HPLC method for simultaneous determination of cefixime trihydrate and sodium benzoate in powder for oral suspension dosage form, Journal of Forensic Science and Criminal Investigation, 2(5). DOI: 10.19080/JFSCI.2017.02.555600.
The United States Pharmacopoeial Convention, Sodium benzoate NF Monograph. (2017). USP40- NF 35, 7876.
World Health Organization (WHO), 24 th May 2018.
Parle, M. & Bansal, N. (2006). Herbal medicines: are they safe? Nature Product Radiance, 5, 6–14. ISSN 0975-1092
Ingle, K.P., Deshmukh, A.G., Padole, D.A., Dudhare, M.S., Moharil, M.P. & Khelurkar, V.C. (2017). Phytochemicals: extraction methods, identification, and detection of bioactive compounds from plant extracts, Journal of Pharmacognosy and Phytochemistry, 6, 32–36. E-ISSN: 2278-4136
Azwanida, N.N. (2015). A review on the extraction methods use in medicinal plants, principle, strength, and limitation, Medicinal and Aromatic Plants Research Journals, 4, 196. DOI: 10.4172/2167-0412.1000196
Pandey, A. & Tripathi, S. (2014). Concept of standardization, extraction, and pre-phytochemical screening strategies for herbal drug, Journal of Pharmacognosy and Phytochemistry. 2, 115–9. ISSN 2278-4136
Doughari, J. H. (2012). Phytochemicals: extraction methods, basic structures and mode of action as potential chemotherapeutic agents (pp. 1-33). Rijeka, Croatia: INTECH Open Access Publisher. ISBN 978-953-51-0296-0
Alhusban, A. A., Ata, S. A., & Shraim, S. A. (2019). The safety assessment of toxic metals in commonly used pharmaceutical herbal products and traditional herbs for infants in Jordanian market. Biological trace element research, 187(1), 307-315. https://doi.org/10.1007/s12011-018-1367-1
ICH. (2005). The International Council for Harmonization of technical requirements for pharmaceuticals for human use (ICH), In Validation of Analytical Procedurers: Text and Methodology (Q2) R1, ICH, Geneva, Switzerland.
FDA- Guidance for Industry, Validation of Analytical Procedurers- Definition and Terminology Final Guidance, FDA, Silver Spring, MD, USA. (2010).
Nair, B. (2001). Final report on the safety assessment of Benzyl Alcohol, Benzoic Acid, and Sodium Benzoate. International Journal of Toxicology, 20, 23-50. https://doi.org/10.1080/10915810152630729
Carocho, M., Barreiro, Filomena & B.M., Ferreira, P.I. (2014). Adding molecules to food, Pros and cons: a review on synthetic and natural food additives. Comprehensive Reviews in Food Science and Food Safety. 13 (4), 377–399. https://doi.org/10.1111/1541-4337.12065
Lee, J.W. (2006). Fit-for-purpose method development and validation for successful biomarker measurement, Pharmaceutical Research, 23(2), 312-328. https://doi.org/10.1007/s11095-005-9045-3
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