Prevalence and Absolute Quantification of NDM-1: a β-Lactam Resistance Gene in Water Compartment of Lakes Surrounding Hyderabad, India

  • Rajeev Ranjan Department of Civil Engineering, Indian Institute of Technology Hyderabad, India
  • Shashidhar Thatikonda Department of Civil Engineering, Indian Institute of Technology Hyderabad, India
Keywords: Antibiotic Resistance, NDM-1, Lake Water, qPCR, Hierarchical Clustering


NDM-1(New Delhi Metallo-beta-lactamase-1) is considered an emerging environmental contaminant, which causes severe hazards for public health. The abuse of antimicrobials for public health and veterinary use could favor the proliferation of resistance in bacteria. In this study, screening and absolute quantification of the NDM-1 in 17 water samples collected from a different sampling location surrounding Hyderabad, India performed using a real-time quantitative polymerase chain reaction (qPCR). Absolute quantification achieved by running the isolated DNA (Deoxy-ribonucleic acid) samples from different water bodies in triplicate with the known standards of the NDM-1 and results reported as gene copy number/ng(nanogram) of template DNA. All collected samples had shown a positive signal for the NDM-1 during qPCR analysis. Among the tested samples, the highest gene copy number/ng of template DNA was observed in the Mir Alam tank (985.74)Further, based on pollution sources and observed data, a hierarchical clustering analysis was performed to categorize the different sampling locations and results presented in the form of a dendrogram.

Author Biography

Shashidhar Thatikonda, Department of Civil Engineering, Indian Institute of Technology Hyderabad, India

Professor, Department of Civil Engineering, Indian Institute of Technology Hyderabad, India


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How to Cite
Ranjan, R., & Thatikonda, S. (2021). Prevalence and Absolute Quantification of NDM-1: a β-Lactam Resistance Gene in Water Compartment of Lakes Surrounding Hyderabad, India. Journal of Applied Science & Process Engineering, 8(1), 700-711.