Antifungal Properties of Selected Medicinal Plant Species Against Fusarium spp. – A Preliminary Study
DOI:
https://doi.org/10.33736/bjrst.1202.2018Abstract
Usage of synthetic fungicides has inevitably been one of the agricultural practices in combating crop pathogens and maintaining the quality of production. Although fungicides have been proven to be profoundly effective, excessive and frequent reliance on these synthetic fungicides have caused negative impacts to the environment and human health. Besides that, indiscriminate use of fungicides may lead to the development of resistant strains of pathogenic fungi. The need to find an alternative solution to synthetic fungicides has led to the interest in finding plant-based fungicides. This study aimed to test the antifungal properties of plant extracts from 13 different medicinal plant species towards plant pathogenic fungi. Absolute methanol was used as a solvent to extract the secondary metabolites from the different plant species. The effect of methanolic crude extract at different concentrations (500 g/ml, 250 g/ml and 100 g/ml), from different medicinal plant species, were tested on the growth of two Fusarium spp., FsB and FsP. The assay showed that the methanolic crude extract from six plant species viz. Alpinia galanga, Annona muricata, Archidendron jiringa, Nephelium lappaceum, Polygonum minus and Artocarpus hybrid (Nanchem) had successfully inhibit the radial mycelial growth of either FsB or FsP, or both. The assay suggested that the six plant species have antifungal properties towards the crop pathogenic fungi tested.
Keywords: antimicrobial, Fusarium, plant extracts, methanolic extracts, biofungicides
References
Báidez, A.G., Gómez, P., Del Río, J.A. & Ortuño, A. (2007). Dysfunctionality of the xylem in Olea europaea L. plants associated with the infection process by Verticillium dahliae Kleb. Role of phenolic compounds in plant defense mechanism. Journal of Agricultural and Food Chemistry, 55(9): 3373-3377.
Balasundram, N., Sundram, K. & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1): 191-203.
Bennett, R.N. & Wallsgrove, R.M. (1994). Secondary metabolites in plant defence mechanisms. New Phytologist, 127(4): 617-633.
Bravo, L. (1998). Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Reviews, 56(11): 317 -333
https://doi.org/10.1111/j.1753-4887.1998.tb01670.x
Brent, K.J. & Holloman, D.W. (2007). Fungicide resistance in crop pathogens: How can it be managed? Fungicide Resistance Action Committee, Monograph 1, Global Crop Protection Federation.
Brito-Argáez, L., Moguel-Salazar, F., Zamudio, F., González-Estrada, T. & Islas-Flores, I. (2009). Characterization of a Capsicum chinense seed peptide fraction with broad antibacterial activity. Asian Journal of Biochemistry, 4(3): 77-87.
Chauhan, A. & Mittu, B. (2015). Phyto-chemical screening and anti listerial activity of Annona Muricata (L) leaf extract. Journal of Chromatography & Separation Techniques, 6(3): 269.
Clinical and Laboratory Standards Institute (CLSI) (2012). Performance standards for antimicrobial disk susceptibility tests; Approved standard - Eleventh edition. CLSI document M02-A11. Wayne, PA: Clinical and Laboratory Standards Institute.
Cowan, M.M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4): 564-582.
Francis, R. & Keinath, A. (2010). Biofungicides and Chemicals for Managing Diseases In Organic Vegetable Production. CLEMSON Cooperative Extencion Information Leaflet. Pp. 88.
George, V.C., Kumar, D.N., Suresh, P.K. & Kumar, R.A. (2015). Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts. Journal of Food Science and Technology, 52(4): 2328-2335.
Hossain, F., Ali, O., D'Souza, U.J. & Naing, D.K.S. (2010). Effects of pesticide use on semen quality among farmers in rural areas of Sabah, Malaysia. Journal of Occupational Health, 52(6): 353-360.
Nuttaporn, S. (2007). Isolation and identification of galangin and other compounds from Alpinia galanga linnaeus willd and Alpinia officinarum hance (MSc thesis), Suranaree University of Technology.
Peraica, M., Radic, B., Lucic, A. & Pavlovic, M. (1999). Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization, 77(9): 754-766.
Pereira, A.P., Ferreira, I.C., Marcelino, F., Valentão, P., Andrade, P.B., Seabra, R., Estevinho, L., Bento, A. & Pereira, J.A. (2007). Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrançosa) leaves. Molecules, 12(5): 1153-1162.
Qader, S.W., Abdulla, M.A., Chua, L.S. & Hamdan, S. (2012). Potential bioactive property of Polygonum minus Huds (kesum) review. Scientific Research and Essays, 7(2): 90-93.
Seo, J.W., Cho, S.C., Park, S.J., Lee, E.J., Lee, J.H., Han, S.S., Pyo, B.S., Park, D.H. & Kim, B.H. (2013). 1′-Acetoxychavicol acetate isolated from Alpinia galangal ameliorates ovalbumin-induced asthma in mice. PLoS One, 8(2): e56447.
Thitilertdecha, N., Teerawutgulrag, A. & Rakariyatham, N. (2008). Antioxidant and antibacterial activities of Nephelium lappaceum L. extracts. LWT-Food Science and Technology, 41(10): 2029-2035.
Wightwick, A., Walters, R., Allinson, G., Reichman, S. & Menzies, N. (2010). Environmental risks of fungicides used in horticultural production systems. In Carisse O. (Ed), Fungicides, InTech. Pp. 273-304.
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