@article{BABAI_KIRA_MOHD SINANG_MAURICE BILUNG_2020, title={Occurrences of Vibrio parahaemolyticus in Retailed Freshwater Fish}, volume={3}, url={https://publisher.unimas.my/ojs/index.php/TUR/article/view/2181}, DOI={10.33736/tur.2181.2020}, abstractNote={<p><em>Vibrio parahaemolyticus</em> is a causative agent of foodborne outbreaks associated with the consumption of raw or under-cooked seafood. This study aimed to quantify and detect the occurrence of <em>V. parahaemolyticus</em> in freshwater fish by performing Most Probable Number (MPN) method in combination with Polymerase Chain Reaction (PCR). In this study, a total of 20 red tilapia (<em>Oreochromis</em> sp.) were collected from nearby local wet markets. Polymerase Chain Reaction (PCR) assay targeting the <em>tox</em>R gene in <em>V. parahaemolyticus</em> was performed, with the expected DNA amplification size of 368 bp. MPN analysis showed that the estimated microbial load of <em>V. parahaemolyticus </em>were more than 1100 MPN/g. The result of the PCR assay confirmed the presence of <em>V. parahaemolyticus </em>in 90% of the isolates. This positive detection elucidated the presence of food-borne bacteria in freshwater fish from local wet-market which may affect not only the health of fish stocks but also raise public health concerns.</p&gt;}, number={1}, journal={Trends in Undergraduate Research}, author={BABAI, JESMIE and KIRA, ROSDI and MOHD SINANG, FAZIA and MAURICE BILUNG, LESLEY}, year={2020}, month={May}, pages={a1-4} }