Occurrences of Vibrio parahaemolyticus in Retailed Freshwater Fish



Vibrio parahaemolyticus is a causative agent of foodborne outbreaks associated with the consumption of raw or under-cooked seafood. This study aimed to quantify and detect the occurrence of V. parahaemolyticus in freshwater fish by performing Most Probable Number (MPN) method in combination with Polymerase Chain Reaction (PCR). In this study, a total of 20 red tilapia (Oreochromis sp.) were collected from nearby local wet markets. Polymerase Chain Reaction (PCR) assay targeting the toxR gene in V. parahaemolyticus was performed, with the expected DNA amplification size of 368 bp. MPN analysis showed that the estimated microbial load of V. parahaemolyticus were more than 1100 MPN/g. The result of the PCR assay confirmed the presence of V. parahaemolyticus in 90% of the isolates. This positive detection elucidated the presence of food-borne bacteria in freshwater fish from local wet-market which may affect not only the health of fish stocks but also raise public health concerns.


Apun, K., Asiah, M. Y., & Jugang, K. (1999). Distribution of bacteria in tropical freshwater fish and ponds. International Journal of Environmental Health Research, 9, 285-292.


Feldhusen, F. (2000). The role of seafood in bacterial food borne diseases. Microbes and Infection, 2, 1651-1660.


Givens, C. E., Bowers, J. C., DePaola, A., Hollibaugh, J. T., & Jones, J. L. (2014). Occurrence and distribution of Vibrio vulnificus and Vibrio parahaemolyticus - potential role for fish, oyster, sediment and water. Letters in Applied Microbiology, 58, 503-510.


Huehn, S., Eichhorn, C., Urmersbach, S., Breidenbach, J., Bechlars, S., Bier, N., Alter, T., Bartelt, E., Frank, C., Oberheitmann, B., Gunzer, F., Brennholt, N., Böer, S., Appel, B., Dieckmann, R., & Strauch, E. (2014). Pathogenic Vibrios in environmental, seafood and clinical sources in Germany. International Journal of Medical Microbiology, 304, 843-850.


Institute of Food and Agriculture Sciences, IFAS (2009). Preventing food borne and nonfood borne illness. Retrieved October 3, 2012, from http://edis.ifas.ufl.edu.

Kaysner, C., DePaola, A. J., & Jones, J. (2004). Vibrio. In Bacteriological Analytical Manual. Retrieved March 25, 2019, from http://www.fda.gov/Food/scienceResearch/LaboratoryMethods/BacteriologicalAnalytical ManualBAM/ucm070830.htm.

Letchumanan, V., Yin, W. F., Lee, L. H., & Chan, K. G. (2015). Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shrimps in Malaysia. Frontiers in Microbiology, 6, 33.


Meador, C. E., Parsons, M. M., Bopp, C. A., Gerner-Smidt, P., Painter, J. A., & Vora, G. J. (2007). Virulence gene-and pandemic group-specific marker profiling of clinical Vibrio parahaemolyticus isolates. Journal of Clinical Microbiology, 45(4), 1133-1139.


Noorlis, A., Ghazali, F. M., Cheah, Y. K., Tuan Zainazor, T. C., Ponniah, J., Tunung, R., Tang, J. Y. H., Nishibuchi, M., Nakaguchi, Y., & Son, R. (2011). Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level. International Food Research Journal, 18, 689-695.

Novoslavskij, A., Margarita, T., Inga, E., Olga, V., Vadims, B., & Aivars, B. (2016). Major foodborne pathogens in fish and fish products: a review. Annals of Microbiology, 66, 1-15.


Novotny, L., Dvorska, L., Lorencova, A., Beran, V., & Pavlik, I. (2004). Fish: a potential source of bacterial pathogens for human beings. Veterinary Medicine, 49(9), 343-358.


Pillay, T. V. R. (1990). Fish and public health and disease. In Aquaculture, principles and practices (pp. 174-215). Oxford, UK: Fishing News Books.

Southeast Asian Fisheries Development Center, SEADEC. (2015). Fisheries country profile: Malaysia. Retrieved June 10, 2018, from http://www.seafdec.org/fisheries-country-profileMalaysia/.

Tunung, R., Margaret, S. P., Jeyaletchumi, P., Chai, L. C., Zainanor, T. C., Ghazali, F. M., Nakaguchi, Y., Nishibuchi, M., & Son, R. (2010). Prevalence and quantification of Vibrio in raw salad vegetables at retail level. Journal of Microbiology and Biotechnology, 20(2), 391-396.


Yang, Z., Jiao, X., Zhou, X., Cao, G., Fang, W., & Gu, R. (2008). Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried and salted seafood products in two coastal areas of eastern China. International of Food Microbiology, 125, 279-285.


How to Cite
BABAI, J., KIRA, R., MOHD SINANG, F., & MAURICE BILUNG, L. (2020). Occurrences of Vibrio parahaemolyticus in Retailed Freshwater Fish. Trends in Undergraduate Research, 3(1), a1-4. https://doi.org/10.33736/tur.2181.2020
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