Trace Element Assessment in Fingernails of Adult Females

Authors

  • LAURA DINES NGAU
  • BOON SIONG WEE
  • NAZARATUL ASHIFA ABD SALIM

DOI:

https://doi.org/10.33736/tur.1135.2018

Abstract

Trace elements play a significant role in giving nutritional benefits to the body because they act as essential cofactors for all physiological processes. However, there are some trace elements which may bring more harm than good when entering the human body. Due to its ability to incorporate trace elements in an amount that is proportional to an individual’s dietary intake and environmental exposure, human fingernails are suitable biomarkers in assessing the health status of an individual as they reflect on the trace element concentration present in the body. This study has analysed fingernail samples of 23 adult females residing in Kuching and Kota Samarahan, Sarawak, Malaysia for four elements, namely Cd, Cu, Pb and Zn. By using flame atomic absorption spectroscopy (FAAS), the mean elemental concentrations found in fingernail samples of research participants were 171.8 ± 33.8 μg/g for Zn, 27.8 ± 14.8 μg/g for Cu and 2.64 ± 0.94 μg/g for Pb. Cd concentrations were not able to be detected as they were below the detection limits. A standard reference material, NIST 1568b Rice Flour was used to verify the methods used in elemental analysis using FAAS. Independent t-test which was used to compare the means of Zn and Cu between vegetarians and non-vegetarians showed no significant differences for both elements. Moreover, correlation analysis showed negative correlations between Cu/Zn pair and Pb/Zn pair, whereas significant positive correlation was obtained for Cu/Pb pair. The overall data from this study showed good agreements with data obtained from studies in other countries. Therefore, the current data in this study represents the latest background elemental concentrations in fingernails of the residents in Kuching and Kota Samarahan, Sarawak.
Keywords: Adult females, fingernails, flame atomic absorption spectroscopy (FAAS), trace elements

References

Abdulrahman, F. I., Akan, J. C., Chellube, Z. M., & Waziri, M. (2012). Levels of heavy metals in human hair and nail samples from Maiduguri Metropolis, Borno State, Nigeria. World Environment, 2(4), 81-89.

https://doi.org/10.5923/j.env.20120204.05

Afridi, H. I., Kazi, T. G., Kazi, N., Jamali, M. K., Arain, M. B., Jalbani, N., Baig, J. A., & Sarfraz, R. A. (2008). Evaluation of status of toxic metals in biological samples of diabetes mellitus patients. Diabetes Research and Clinical Practice, 80, 280-288.

https://doi.org/10.1016/j.diabres.2007.12.021

Ashish, B., Neeti, K., & Himanshu, K. (2013). Copper toxicity: A comprehensive study. Research Journal of Recent Sciences, 2, 58-67.

Ashraf, W., Jaffar, M., & Mohammad, D. (1994). Age and sex dependence of selected trace metals in scalp hair of urban population of Pakistan. Science of Total Environment, 151, 227-233.

https://doi.org/10.1016/0048-9697(94)90471-5

Ashraf, W., Jaffar, M., Anwer, K., & Ehsan, U. (1995). Age- and sex-based comparative distribution of selected metals in the scalp hair of an urban population from two cities in Pakistan. Environmental Pollution, 87, 61-64.

https://doi.org/10.1016/S0269-7491(99)80008-6

Angerer, J., Aylward, L. L., Hays, S. M., Heinzow, B., & Wilhelm, M. (2011). Human biomonitoring assessment values: Approaches and data requirements. International Journal of Hygiene and Environmental Health, 214, 348-360.

https://doi.org/10.1016/j.ijheh.2011.06.002

Bost, M., Houdart, S., Oberli, M., Kalonji, E., Huneau, J. F., & Margaritis, I. (2015). Dietary copper and human health: Current evidence and unresolved issues. Journal of trace elements in medicine and biology, 35, 107-115.

https://doi.org/10.1016/j.jtemb.2016.02.006

Chaudhary, K., Ehmann, W. D., Rengan, K., & Markesbery, W. R. (1995). Trace element correlations with age and sex in human fingernails. Journal of Radioanalytical and Nuclear Chemistry, 195(1), 51-56.

https://doi.org/10.1007/BF02036472

Ebrahim, A. M., Eltayeb, M., Benker, B., Grill, P., Attahir, M., Osman, A., Elsadig, M., & Michalke, B. (2011). Study on some trace elements contents in serum and nail samples obtained from Sudanese subjects. Biological Trace Element Research, 144, 225-233.

https://doi.org/10.1007/s12011-011-9076-z

Esteban, M., & Castano, A. (2008). Non-invasive matrices in human biomonitoring: A review. Environment International, 35, 438-449.

https://doi.org/10.1016/j.envint.2008.09.003

Fadayon, F., Abdollahi, A., Nia, S. R., & Ostovar, R. (2013). Relationship between the level of zinc, lead, cadmium, nickel and chromium in hair of people with diabetes. Web of Conferences, 1. doi.org/10.1051/e3sconf/20130141012

https://doi.org/10.1051/e3sconf/20130141012

Ghazali, A. R., Kamarulzaman, F., Normah, C. D., Ahmad, M., Ghazali, S. E., Ibrahim, N., & Razali, R. (2013). Levels of metallic elements and their potential relationships to cognitive function among elderly from Federal Land Development Authority (FELDA) settlement in Selangor Malaysia. Biological Trace Element Research, 153, 16-21.

https://doi.org/10.1007/s12011-013-9642-7

Ghazali, A. R., Razak, N. E. A., Othman, M. S., Othman, H., Ishak, I., Lubis, S. H., Mohammad, N., Hamid, Z., A., Harun, Z., Kamarulzaman, F., & Abdullah, R. (2012). Study of heavy metal levels among farmers of Muda Agricultural Development Authority, Malaysia. Journal of Environmental and Public Health, 2012. doi:10.1155/2012/758349

https://doi.org/10.1155/2012/758349

Gholami, M., Behkami, S., Zain, S. M., & Bakirdere, S. (2016). A simple design for microwave assisted digestion vessel with low reagent consumption suitable for food and environmental samples. Scientific Reports, 6. doi: 10.1038/srep37186

https://doi.org/10.1038/srep37186

Goulle, J. P., Saussereau, E., Mahieu, L., Bouige, D., Groenwont, D., Guerbet, M., & Lacroix, C. (2009). Application of inductively coupled plasma mass spectrometry mulitielement analysis in fingernail and toenail as a biomarker of metal exposure. Journal of Analytical Toxicology, 33, 92-98.

https://doi.org/10.1093/jat/33.2.92

Hunt, J. R. (2003). Bioavailability of iron, zinc and other trace minerals from vegetarian diets. The American Journal of Clinical Nutrition, 78, 633-639.

https://doi.org/10.1093/ajcn/78.3.633S

Kanabrocki, E. L., Kanabrocki, J. A., Greco, J., Kaplan, E., & Oester, Y. T. (1979). Instrumental analysis of trace elements in thumbnails of human subjects. The Science of the Total Environment, 13, 131-140.

https://doi.org/10.1016/0048-9697(79)90067-6

King, J. C., Shames, D. M., & Woodhouse, L. R. (2000). Zinc homeostasis in humans. The Journal of Nutrition, 130(5), 1360-1366.

https://doi.org/10.1093/jn/130.5.1360S

Lech, T., & Sadlik, J. K. (2007). Copper concentration in body tissues and fluids in normal subjects of Southern Poland. Biological Trace Element Research, 118, 10-15.

https://doi.org/10.1007/s12011-007-0014-z

Li, Y., Zou, X., Lv, J., Yang, L., Li, H., & Wang, W. (2012). Trace elements in fingernails of healthy Chinese centenarians. Biological Trace Element Research, 145, 158- 165.

https://doi.org/10.1007/s12011-011-9187-6

Mahler, D. J., Scott, A. F., Walsh, J. R., & Haynie, G. (1970). A study of trace metals in fingernails and hair using neutron activation analysis. Journal of Nuclear Medicine, 11, 739-742.

Mehra, R., & Juneja, M. (2005). Fingernails as biological indices of metal exposure. Journal of Bioscience, 30, 253-257.

https://doi.org/10.1007/BF02703706

Mehra, R., & Thakur, A. S. (2016). Relationship between lead, cadmium, zinc, manganese and iron in hair of environmentally exposed subjects. Arabian Journal of Chemistry, 9, 1214-1217.

https://doi.org/10.1016/j.arabjc.2012.01.014

Momen, A. A., Khalid, M. A. A., Elsheikh, M. A. A., & Ali, D. M. H. (2015). Trace elements in scalp hair and fingernails as biomarkers in clinical studies. Journal of Health Specialties, 3(1), 28-34.

https://doi.org/10.4103/1658-600X.150760

Nowak, B., & Chmielnicka, J. (2000). Relationship of lead and cadmium to essential elements in hair, teeth, and nails of environmentally exposed people. Ecotoxicology and Environmental Safety, 62, 213-228.

https://doi.org/10.1006/eesa.2000.1921

Nyambura, J. N. (2012). Determination of levels of essential elements, lead and lead risk exposure factors in adult males using fingernails and scalp hair (master's thesis). Kenyatta University, Nairobi, Kenya.

Olabanji, S. O., Ajose, O. A., Makinde, N. O., Buoso, M. C., Ceccato, D., DePoli, M., & Moschini, G. (2005). Characterization of human fingernail elements using PIXE techniques. Nuclear Instruments and Methods in Physics Research Section B, 240, 895-907.

https://doi.org/10.1016/j.nimb.2005.06.206

Parizanganeh, A., Zamani, A., Bijnavand, V., & Taghilou, B. (2014). Human nail usage as a bio-indicator in contamination monitoring of heavy metals in Dizajabaad, Zanjan province-Iran. Journal of Environmental Health Science & Engineering, 12, 147-155.

https://doi.org/10.1186/s40201-014-0147-x

Rodushkin, I., & Axelsson, M. D. (2000). Application of double focusing sector field ICP- MS for multielemental characterization of human hair and nails. Part II: A study of the inhabitants of northern Sweden. Science of Total Environment, 262, 21-36.

https://doi.org/10.1016/S0048-9697(00)00531-3

Samantha, G., Sharma, R., Roychowdhury, T., & Chakraborti, D. (2004). Arsenic and other elements in hair, nails and skin-scales of arsenic victims in West Bengal, India. Science of the Total Environment, 326, 33-47.

https://doi.org/10.1016/j.scitotenv.2003.12.006

Shrivastava, A., & Gupta, V. B. (2011). Methods for the determination of limit of detection and limit of quantification of the analytical methods. Chronicles of Young Scientists, 2(1), 21-25.

https://doi.org/10.4103/2229-5186.79345

Skalny, A. V., Skalnaya, M. G., Tinkov, A. A., Serebryanksky, E. P., Demidov, V. A., Lobanova, Y. N., Grabeklis, A. R. & Nikonorov, A. A. (2015). Reference values of toxic trace elements content in occupationally non-exposed Russian population. Environmental Toxicology and Pharmacology, 409(1), 18-21.

https://doi.org/10.1016/j.etap.2015.05.004

Strachan, S. (2010). Trace elements. Current anaesthesia & Critical Care, 21, 44-48.

https://doi.org/10.1016/j.cacc.2009.08.004

Waseem, A., & Arshad, J. (2016). A review of human biomonitoring studies of trace elements in Pakistan. Chemosphere, 163, 153-176.

https://doi.org/10.1016/j.chemosphere.2016.08.011

Wee, B. S., & Ebihara, M. (2017). Neutron activation analysis and assessment of trace elements in fingernail from residents of Tokyo, Japan. Sains Malaysiana, 46(4), 605-613.

https://doi.org/10.17576/jsm-2017-4604-13

World Health Organization (WHO). (2015). Human biomonitoring: Facts and figures. Retrieved January 15, 2018, from http://www.euro.who.int/data/assets/pdf_file/0020/276311/Human-biomonitoring-facts-figures-en.pdf.

Yoshida, M., Ogi, N., & Iwashita, Y. (2011). Estimation of mineral and trace element intake in vegans living in Japan by chemical analysis of duplicate diets. Health, 3, 672-676.

https://doi.org/10.4236/health.2011.311113

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Published

2018-12-10

How to Cite

NGAU, L. D., WEE, B. S., & SALIM, N. A. A. (2018). Trace Element Assessment in Fingernails of Adult Females. Trends in Undergraduate Research, 1(1), a8–15. https://doi.org/10.33736/tur.1135.2018

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Section

Resource Science and Technology