Influence of Temperature and Oxygen Injection on Population Growth of Marine Rotifers, Brachionus plicatilis
Keywords:
Aquaculture, Hatchery, Live feed, Mariculture, Population growth, RotiferAbstract
Rotifers (Brachionus plicatilis, Brachionidae) are critical live feed for marine, crustaceans, and ornamental fish during their early developmental stages. This study investigated the effects of temperature and oxygen injection on rotifer population growth. A five-day experiment was conducted with nine treatments combining temperatures (26 °C, 28 °C, and 30 °C) and oxygen injection frequencies (0, 2, 4, and 6 times/day). Each treatment was triplicated, with 27 experimental jars containing 4 L of water, a filter, and aeration at 200 mL/min. Commercial Nannochloropsis sp. was used as feed through automated feeders. The results show significant effects of temperature and oxygen injection on population density, growth rate, and egg production (p<0.01). Statistical analysis was conducted using two-way ANOVA to assess the significance of main and interaction effects. The highest population density (308 individuals/mL) and growth rate (1.14 day⁻¹) were observed at 30 °C with 6 oxygen injections/day, followed by 256 individuals/mL with 4 injections/day. The lowest density (32 individuals/mL) and growth rate (0.62 day⁻¹) were recorded at 26 °C with 4 injections/day. Egg production was highest (27.09 ± 2.49%) at 30 °C with 6 injections/day. These findings indicate the critical role of temperature and oxygen injection frequency for optimizing rotifer production for aquaculture.
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