Optimization of Lactic Acid Fermentation Conditions for the Production of Antibacterial Peptides Targeting Pantoea spp. for Rice Leaf Blight Control
Keywords:
Antimicrobial Peptides, Bioactive Molecules, Lactic Acid Fermentation, Pantoea spp., Rice Disease ControlAbstract
This study aimed to optimize the production of antibacterial peptides from Bactronophorus thoracites via lactic acid fermentation, specifically focusing on Pantoea species to manage rice leaf blight. The main goal was to investigate sustainable and environmentally friendly approaches to address this agricultural disease using bioactive compounds derived from marine sources. The fermentation process was refined using Response Surface Methodology (RSM), producing highly reliable results confirmed by the analysis of variance (ANOVA) and strong determination coefficients (R² = 0.9952 for Pantoea ananatis and R² = 0.9967 for Pantoea stewartii). The optimized parameters included a 4-day fermentation duration, a 3% (w/v) glucose concentration, and a 0.92% (w/v) solid-to-water ratio. These conditions closely matched predictive models and were further validated by a residual standard error (RSE) of less than 5%. The study identified the minimum inhibitory concentration (MIC) of the bioactive peptides, determining that 125 µg/mL was effective against the target bacteria. The hydrolysates produced in this study show promise as a natural method to control rice leaf blight and may have broader applications in agricultural disease management. This research highlights the potential of optimized lactic acid fermentation to produce effective antimicrobial agents, contributing to sustainable agriculture and offering new biotechnological strategies for plant disease control.
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