Impact of Various Pre-Treatments on the Lignocellulosic Compositions of Sarawak ‘Paun’ Pineapple Leaf Waste
Keywords:
bioconversion, compositional analyses, hydrochloric acid, pineapple leaves, pre-treatmentsAbstract
Pre-treatment of lignocellulosic biomass is a crucial step in breaking down the complex structure of the plant`s cell wall to enhance the availability and digestibility of cellulose for bioconversion process of the biomass to value-added products. The overall efficiency of the process designed to convert lignocelluloses also lies on an accurate determination of compositions of the lignocellulosic substrate. In this study, local species of Sarawak ‘Paun’ pineapple leaves collected from Simunjan, Sarawak, were subjected to various pre-treatment methods including thermal treatment, acidic treatments and alkaline treatments. Compositional analyses of the raw and pre-treated leaves were conducted through a gravimetric method to study the effect of different methods of pre-treatments in altering the lignocellulosic compositions of the pineapple leaf wastes focusing on the hemicellulose, lignin and cellulose content. As the main purpose of a pre-treatment method focuses on its ability and efficiency to disintegrate the biomass complex structure, especially, in reducing lignin content for higher cellulose accessibility of enzymes in enzymatic hydrolyses. This study suggested that pre-treatment with 1.5% (v/v) hydrochloric acid solution displayed the most notable change to the lignocellulose contents of the leaves as highest cellulose content (51.5% w/w) and lowest amount of lignin (10.3% w/w) were recorded, compared to that of other pre-treatment methods. These findings may provide a better understanding for future research in designing a suitable biochemical process with enhanced enzymatic digestibility of cellulose present in the pineapple leaves to yield wealth-added products.
References
Aili Hamzah, A.F., Hamzah, M.H., Che Man, H., Jamali, N.S., Siajam, S.I. & Ismail, M.H. (2021). Recent updates on the conversion of pineapple waste (Ananas comosus) to value-added products, future prespectives and challenges. Agronomy, 11: 2221. DOI: 10.3390/agronomy11112221
Amin, F.R., Khalid, H., Zhang, H., Rahman, S.U., Zhang, R., Liu, G. & Chen, C. (2017). Pre-treatment methods of lignocellulosic biomass for anaerobic digestion. AMB Express, 7(72): 1-12. DOI: 10.1186/s13568-017-0375-4
Ariffin, K.K., Masngut, N., Seman, M.N.A., Saufi, S.M., Jamek, S. & Sueb, M.S.M. (2020). Dilute acid hydrolysis pretreatment for sugar and organic acid production from pineapple residues. IOP Conference Series: Materials Science and Engineering, 991: 1-9. DOI: 10.1088/1757-899X/991/1/012057
Awogbemi, O. & Kallon, D.V.V. (2022). Pretreatment techniques for agricultural waste. Case Studies in Chemical and Environmental Engineering, 6: 1-9. DOI: 10.1016/j.cscee.2022.100229
Awoyale, A.A. & Lokhat, D. (2021). Experimental determination of the effects of pretreatment on selected Nigerian lignocellulosic biomass in bioethanol production. NatureResearch, 11(2021): 557. DOI: 10.1038/s41598-020-78105-8
Ayeni, A.O., Adeeyo, O.A., Oresegun, O.M. & Oladimeji, T.E. (2015). Compositional analysis of lignocellulosic materials: evaluation of an economically viable method suitable for woody and non-woody biomass. American Journal of Engineering Research (AJER), 4(4): 14-19.
Bansal, N., Tewari, R., Soni, R. & Soni, S.K. (2012). Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues. Waste Management, 32(7): 1341–1346. DOI: 10.1016/j.wasman.2012.03.006
Cho, E.J., Trinh, L.T.P., Song, Y., Lee, G.Y. & Bae, H.J. (2019). Bioconversion of biomass waste into high value chemicals. Bioresource Technology, 2019: 1-48. DOI: 10.1016/j.biortech.2019.122386
Corbin, K.R., Hsieh, Y.S.Y., Betts, N.S., Byrt, C.S., Henderson, M., Stork, J., DeBolt, S., Fincher, G.B. & Burton, R.A. (2015). Grape marc as a source of carbohydrates for bioethanol: chemical composition, pre-treatment and saccharification. Bioresource Technology, 2015: 1-34. DOI: 10.1016/j.biortech.2015.06.030
Fadeyi, A.E., Akiode, S.O., Emmanuel, S.A. & Falavi, O.E. (2020). Compositional analysis and characterization of lignocellulosic biomass from selected agricultural wastes. Journal of Science and Mathematics Letters, 8(1): 48-56. DOI: 10.37134/jsml.vol8.1.6.2020
Ferreira, S.L.C., Silva, L.O.B., de Santana, F.A., Junior, M.M.S., Matos, G.D. & dos Santos, W.N.L. (2013). A review of reflux systems using cold finger for sample preparation in the determination of volatile elements. Microchemical Journal, 106(2013): 307-310. DOI: 10.1016/j.microc.2012.08.015
Hu, L.S., Fang, X.Z., Du, M.H., Luo, F. & Guo, S. (2020). Hemicellulose-based polymers processing and application. American Journal of Plant Sciences, 11: 2066-2079. DOI: 10.4236/ajps.2020.1112146
Iram, A., Cekmecelioglu, D. & Demirci, A. (2020). Ideal feedstock and fermentation process improvements for the production of lignocellulolytic enzymes. Process, 9(38): 1-26. DOI: 10.3390/pr9010038
Iroba, K.L., Tabil, L.G., Dumonceaux, T. & Baik, O. (2013). Effect of alkaline pretreatment on chemical composition of lignocellulosic biomass using radio frequency heating. Biosystems Engineering, 116: 385-398. DOI: 10.1016/j.biosystemseng.2013.09.004
Kim, J.S., Lee, Y.Y. & Kim, T.H. (2016). A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresource Technology, 2015: 1-7. DOI: 10.1016/j.biortech.2015.08.085
Lobo, F.C.M., Franco, A.R., Fernandes, E.M. & Reis, R.L. (2021). An overview of the antimicrobial properties of lignocellulosic materials. Molecules, 26: 1749. DOI: 10.3390/molecules26061749
Lodha, A., Pawar, S. & Rathod V. (2020). Optimised cellulase production from fungal co-culture of Trichodetma reesei NCIM 1186 and Penicillium citrinum NCIM 768 under solid state fermentation. Journal of Environmental Chemical and Engineering, 8(5): 1-6. DOI: 10.1016/j.jece.2020.103958
Maisyarah, A., Lim, J.S., Ani, F.N., Hashim, H. & Ho, W.S. (2019), Characteristics of cellulose, hemi-cellulose and lignin of MD2 pineapple biomass. Chemical Engineering Transaction, 72: 79–84. DOI: 10.3303/CET1972014
Nashiruddin, N.I., Mansor, A.F., Rahman, R.A., Ilias, R.M. & Wan Yussof, H. (2020). Process parameter optimization of pretreated pineapple leaves fiber for enhancement of sugar recovery. Industrial Crops and Products, 152(112514): 1-8. DOI: 10.1016/j.indcrop.2020.112514
Oriez, V., Peydecastaing, J. & Pontalier, P. (2019). Lignocellulosic biomass fractionation by mineral acids and resulting extract purification processes: conditions, yields, and purities. Molecules, 24(2019): 4273. DOI: 10.3390/molecules24234273
Pendse, D.S., Deshmukh, M. & Pande, A. (2023). Different pre-treatments and kinetic models for bioethanol production from lignocellulosic biomass: A review. Heliyon, 9: 1-15. DOI: 10.1016/j.heliyon.2023.e16604
Rajesh, B.J., Sugitha, S., Kavitha, S., Yukesh, K.R., Merrylin, J. & Kumar, G. (2021). Lignocellulosic biomass pretreatment for enhanced bioenergy recovery: Effect of lignocelluloses recalcitrance and enhancement strategies. Frontiers in Energy Research, 9: 1-17. DOI: 10.3389/fenrg.2021.646057
Reddy, N. & Yang, Y. (2005). Biofibers from agricultural byproducts for industrial applications. TRENDS in Biotechnology, 23(1): 22-27. DOI: 10.1016/j.tibtech.2004.11.002
Saini, R., Chen, C., Patel, A.K., Saini, J.K., Dong, C. & Singhania, R.R. (2022). Valorization of pineapple leaves waste for the production of bioethanol. Bioengineering, 9(557): 1-9. DOI: 10.3390/bioengineering9100557
Saravanan, P., Muthuvelayudham, R. & Viruthagiri, T. (2013). Enhanced production of cellulase from pineapple waste by response surface methodology. Journal of Engineering, 2013: 1-8. DOI: 10.1155/2013/979547
Shi, F., Wang, Y., Davaritouchaee, M., Yao, Y. & Kang, K. (2020). Directional structure modification of poplar biomass- inspired high efficacy of enzymatic hydrolysis by sequential dilute acid-alkali treatment. ACS Omega, 5: 24780-24789. DOI: 10.1021/acsomega.0c03419
Singh, A., Bajar, S., Devi, A. & Bishnoi, N.R. (2021). Evaluation of cellulase production of Aspergillus niger and Aspergillus heteromorphus under submerged and solid-state fermentation. Environmental Sustainability, 4: 437-442. DOI: 10.1007/s42398-021-00173-x
Sluiter, J.B., Ruiz, R.O., Scarlata, C.J., Sluiter, A.D. & Templeton, D.W. (2010). Compositional analysis of lignocellulosic feedstocks. 1. Review and description of methods. Journal of Agricultural Food Chemistry, 58: 9043-9053. DOI: 10.1021/jf1008023
Tumuluru, J.S., Sokhansanj, S., Wright, C.T. & Boardman, R.D. (2011). A review on biomass torrefaction process and product properties for energy applications. Industrial Biotechnology, 7(5): 384-401. DOI: 10.1089/ind.2011.0014
Usino, D.O., Sar, T., Ylitervo, P. & Richards, T. (2023). Effect of acid pretreatment on the primary products of biomass fast pyrolysis. Energies, 16: 2377. DOI: doi.org/10.3390/en16052377
Vélez-Mercado, M.I., Talavera-Caro, A.G., Escobedo-Uribe, K.M., Sánchez-Muñoz, S., Luévanos-Escareño, M.P., Hernández-Terán, F., Alvarado, A. & Balagurusamy, N. (2021). Bioconversion of lignocellulosic biomass into value added products under anaerobic conditions: Insight into proteomic studies. International Journal of Molecular Sciences, 22(12249): 1-26. DOI: 10.3390/ijms222212249
Widihastuty, Y.R., Sutini & Nur Ramadhani, A. (2021). Optimization of fermentable sugar production from pineapple leaf waste (Ananas comosus [L.] Merr) by enzymatic hydrolysis. Jurnal Presipitasi, 18(1): 73-80. DOI: 10.14710/presipitasi.v18i1.73-80
Yoon, L.W., Ang, T.N., Ngoh, G.C. & Adeline, S.M.C. (2014). Fungal solid-state fermentation and various methods of enhancement in cellulase production. Biomass and Bioenergy, 67: 319-338. DOI: 10.1016/j.biombioe.2014.05.013
Yuliasmi, S., Nerdy & Husnita, A. (2017). Characterization of microcrystalline from pineapple leaf (Ananas comosus L. Merr). IOP Conference Series: Materials Science and Engineering, 180: 1-7. DOI: 10.1088/1757-899X/180/1/012267
Zawawi, D., Mohd Zainuri, M.H., Angzzas S.M.K., Halizah, A. & Ashuvila, M.A. (2014). Exploring of agro waste (pineapple leaf, corn stalk, and napier grass) by chemical composition and morphological study. BioResources, 9(1): 872-880. DOI: 10.15376/biores.9.1.872-880
Zhao, C., Deng, L. & Fang, H. (2018). Mixed culture of recombinant Trichoderma reesei and Aspergillus niger for cellulase production to increase the cellulose degrading capability. Biomass and Bioenergy, 112(2018): 93-98. DOI: 10.1016/j.biombioe.2018.03.001
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