The In Vitro Ovicidal Activity of Cassia alata Methanolic Extracts on Aedes aegypti and Aedes albopictus Eggs
Keywords:
Aedes mosquitoes, ovicidal activity, Cassia alata, extracts, methanolicAbstract
The widespread use of chemical insecticides for controlling Aedes mosquitoes has resulted in the development of insecticide resistance, prompting the need for alternative solutions. Botanical insecticides have gained attention as a promising approach in modern pest control. This study focuses on evaluating the ovicidal effects of methanol extracts from Cassia alata leaves on Aedes mosquitoes. The extracts were produced through the maceration-filtration technique, followed by the preparation of various concentrations (0.05-2.00 mg/ml) These concentrations were then tested to assess their impact on the fertility and egg viability of Aedes aegypti and Aedes albopictus. From the results obtained in the bioassay on egg fertility and viability, a significant difference in fertility was observed between Ae. aegypti and Ae. albopictus (p<0.05, p = 0.044). However, no significant difference was observed in overall egg viability between the two species (p>0.05, p = 0.468). The LC50 and LC90 values for Ae. albopictus were 0.323 mg/ml and 5.280 mg/ml, respectively, which were lower than those for Ae. aegypti 0.560 mg/ml and 11.480 mg/ml. This indicates that, Ae. albopictus is more susceptible to the methanolic C. alata extracts. These findings suggest that C. alata extracts could be a viable alternative to chemical insecticides in mosquito control programs, particularly for targeting Ae. albopictus.
References
Abdullahi, R.A. & Mainul, H. (2020). Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy & Bioallied Sciences, 12(1): 1–10. DOI:10.4103/jpbs.JPBS_175_19
AbuBakar, S., Wan Puteh, S.E., Kastner, R., Oliver, L., Lim, S.H., Hanley, R. & Gallagher, E. (2022). Epidemiology (2012-2019) and costs (2009-2019) of dengue in Malaysia: a systematic literature review. International Journal of Infectious Diseases, 124: 240-247. DOI:10.1016/j.ijid.2022.09.006.
Anabela, B., Helena, J., Vera, H. & Manuel, S. (2020). Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea. Antibiotics, 9(2):48. DOI:10.3390/antibiotics9020048
Andrew, J.M., Manuel, A., Gilberto, F., Veronica, A. & Roberto, B. (2016). Evaluation of Household Bleach as an Ovicide for the Control of Aedes aegypti. Journal of the American Mosquito Control Association, 31(1): 77-84. DOI:10.2987/14-6427R.1
Chew, Y.L., Khor, M.A., Xu, Z., Lee, S.K., Keng, J.W., Sang, S.H., Akowuah, G.A., Goh, K.W., Liew, K.B. & Ming, L.C. (2022). Cassia alata, Coriandrum sativum, Curcuma longa and Azadirachta indica: Food Ingredients as Complementary and Alternative Therapies for Atopic Dermatitis-A Comprehensive Review. Molecules, 27(17): 5475. DOI:10.3390/molecules27175475
Dong-jiang, L., Yong, F., Ling-yun, L., Li-zhao, Z., San-ji, G., Ran, W. & Jin-da, W. (2022). The insecticidal effect of the botanical insecticide chlorogenic acid on Mythimna separata (Walker) is related to changes in MsCYP450 gene expression. Frontiers in Plant Science, 13:1015095. DOI: 10.3389/fpls.2022.1015095
Ephantus, J.M., Jose, L.R., Bruce, Z., Lina, B.F. & Alejandro, P.R. (2018). Ovicidal and Larvicidal Effects of Garlic and Asofoetida Essential Oils Against West Nile Virus Vectors. Journal of Insect Science, 18(2): 43. DOI:10.1093/jisesa/iey036
Fatmawati, S., Yuliana, Purnomo, A.S. & Abu Bakar, M.F. (2020). Chemical constituents, usage and pharmacological activity of Cassia alata. Heliyon, 6(7): e04396. DOI:10.1016/j.heliyon.2020.e04396
Gan, S.J., Leong, Y.Q., Barhanuddin, M.F.H, Wong, S.T., Wong, S.F., Mak, J.W. & Ahmad, R. (2021). Dengue fever and insecticide resistance in Aedes mosquitoes in Southeast Asia: a review. Parasites Vectors, 14: 315. DOI:10.1186/s13071-021-04785-4.
Giovanni, B. (2015). Plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review. Parasitology Research, 114: 3201–3212. DOI:10.1007/s00436-015-4656-z
Govindarajan, M., Mathivanan, T., Elumalai, K., Krishnappa, K. & Anandan, A. (2011). Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Asian Pacific journal of tropical biomedicine, 1(1): 43–48. DOI:10.1016/S2221-1691(11)60066-X
Kamal, A., Bulbuli, K. & Riju, S. (2022). Persistent susceptibility of Aedes aegypti to eugenol. Scientific Reports, 12: 2277. DOI:10.1038/s41598-022-06302-8
Lengai, G.M.W., Muthomi, J.W. & Mbega, E.R. (2020). Phytochemical activity and role of botanical pesticides in pest management for sustainable agricultural crop production, Scientific African, 7: e00239. DOI:10.1016/j.sciaf.2019.e00239.
Lin, L.Q., Chen, Y.H., Tian, Y.F., Zheng, Z.Y., Wu, J.X., Hu, F., Wu, C. & Xie, L.H. (2024). Study on the cross-resistance of Aedes albopictus (Skuse) (Diptera: Culicidae) to deltamethrin and pyriproxyfen, Parasites Vectors, 17: 403. DOI:10.1186/s13071-024-06485-1
Meryem, S.S.D. & Emel, C. (2022). Plant-Based Bioinsecticides for Mosquito Control: Control:Impact on Insecticide Resistance and Disease Transmission. Insects, 13(2):162. DOI:10.3390/insects13020162
Petr, H., Pavel, T. & Marie, S. (2002). Flavonoids-potent and versatile biologically active compounds interacting with cytochromes P450. Chemico-Biological Interactions, 139(1): 1-21. DOI:10.1016/S0009-2797(01)00285-X
Raman Ibrahim, N.B., Puchooa, D., Govinden-Soulange, J. & Facknath, S. (2022). Chemical profiling and biological activity of Cassia abbreviata Oliv. South African Journal of Botany, 146: 325-339. DOI:10.1016/j.sajb.2021.11.004.
Richards, S.L., Byrd, B.D., Reiskind, M.H. & White, A.V. (2020). Assessing Insecticide Resistance in Adult Mosquitoes: Perspectives on Current Methods. Environmental Health Insights, 14. doi:10.1177/1178630220952790
Seng, C.T., Samuel, L., Scholastica, R.B. & Sui, S.L. (2023). In vitro antimicrobial efficacy of Cassia alata (Linn.) leaves, stem, and root extracts against cellulitis causative agent Staphylococcus aureus. BMC Complementary Medicine and Therapies, 23: 85. DOI:10.1186/s12906-023-03914-z
Şengül Demirak, M.Ş. & Canpolat, E. (2022). Plant-Based Bioinsecticides for Mosquito Control: Impact on Insecticide Resistance and Disease Transmission. Insects, 13(2): 162. DOI:10.3390/insects13020162
Senthil-Nathan S. (2020). A Review of Resistance Mechanisms of Synthetic Insecticides and Botanicals, Phytochemicals, and Essential Oils as Alternative Larvicidal Agents Against Mosquitoes. Frontiers in Physiology, 10: 1591. DOI:10.3389/fphys.2019.01591
Simmonds, M.S.J. (2001). Importance of flavonoids in insect–plant interactions: feeding and oviposition. Phytochemistry, 56, (3): 245-252. DOI:10.1016/S0031-9422(00)00453-2.
Soni, S., Gill, V.J.S., Anusheel, Singh, J., Chhabra, J., Gill, G.J.S. & Bakshi, R. (2023). Dengue, Chikungunya, and Zika: The Causes and Threats of Emerging and Re-emerging Arboviral Diseases. Cureus, 15(7): e41717. DOI:10.7759/cureus.41717
Truong, D.H., Dinh, H.N., Nhat, T.A.T., Anh, V.B., Tuong, H.D. & Hoang, C.N. (2019). Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. Journal of Food Quality, 2019(1): 8178294. DOI:10.1155/2019/8178294
World Health Organization (2005). Guidelines and field testing of mosquito larvicides. World Health Organization. https://iris.who.int/handle/10665/69101
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