Morphological and Physiological Development of Pyricularia oryzae Isolates from North-western Region of Sarawak on Different Media under Laboratory Conditions
Growth of Pyricularia oryzae on different media
Rice blast (causal agent: Pyricularia oryzae) is an important disease of rice in Sarawak. Understanding the pathogen’s morphological characteristics, genetic diversity and pathogenicity is important. Having a suitable medium for culturing and maintaining P. oryzae is important to ensure the availability of inoculum or materials under laboratory conditions. Oatmeal agar (OMA) and potato dextrose agar (PDA) are common media used for growing P. oryzae. OMA allows better mycelial growth and better sporulation as compared to PDA. There are also other alternatives such as fresh rice leaf agar and rice straw agar. Although OMA seems to be the best medium, unfortunately the opaqueness of the medium causes difficulty in observing the morphology and growth of mycelia. In addition, it is known that different isolates of P. oryzae will respond differently to different media. This study aims to identify the best media for culturing and maintaining P. oryzae isolates from Sarawak. A total of 14 P. oryzae isolates were characterised for their morphological characteristics, growth rate and sporulation rate using seven growing media. These 14 isolates included seven newly identified isolates in this study and seven isolates from a previous study, which were verified using internal transcribed spacer DNA sequence. The colony surface of the 14 P. oryzae isolates varied on different growing media. The pigmentation of colony surface varied from different shades of grey, translucent light brown, white and colourless. Pyricularia oryzae isolates grew better on OMA and PDA, while OMA was the best for sporulation. These two media can be recommended for culturing and maintaining different P. oryzae isolates under laboratory conditions.
Abed-Ashtiani, F., Kadir, J., Nasehi, A., Hashemian-Rahaghi, S.R., Vadamalai, G. & Rambe, S.K. (2016). Characterisation of Magnaporthe oryzae isolates from rice in Peninsular Malaysia. Czech Journal of Genetics and Plant Breeding, 52(4): 145-156. DOI: 10.17221/102/2016-CJGPB
Altschul, S.F., Gishm, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3): 403-410. DOI: 10.1016/S0022-2836(05)80360-2
Gad, M.A., Dileep, K.B.S., Bora, T.C., Deka, M., Ibrahim, N.A. & Nath, P.D. (2011). Studies on the sporulation and growth of the rice blast fungus Magnaporthe oryzae. Journal of Assam Science Society, 52(2): 24-31.
Gianessi, L.P. (2014). Importance of pesticides for growing rice in South and South East Asia. International pesticide benefit case study, 108: 30-3.
Gohel, N.M. & Chauhan, H.L. (2015). Nutritional and physiological studies on the growth and sporulation of Pyricularia oryzae Cavara isolated from Rice. Trends in Biosciences, 8(22): 6381-6387.
Harun, R. (2015). 13.0 Kajian penandaarasan dan memprospek teknologi pengeluaran padi. In Dardak, R.A., Suntharalingam, C., Suhaimee, S., Masdek, N.R.N.M. & Hashim, F.A.H. (eds.) Laporan Kajian Sosioekonomi 2015. Serdang, Malaysia, Institut Penyelidikan dan Kemajuan Pertanian Malaysia (MARDI). pp. 171-189.
Hosseini-Moghaddam, M.S. & Soltani, J. (2013). An Investigation on the effects of photoperiod, aging and culture media on vegetative growth and sporulation of rice blast pathogen Pyricularia oryzae. Progress in Biological Sciences, 3(2): 135-143. DOI: 10.22059/PBS.2013.35843
Huang, P., Cao, H., Li, Y., Zhu, S., Wang, J., Wang, Q., Liu, X., Lin, F.C. & Lu, J. (2022). Melanin promotes spore production in the rice blast fungus Magnaporthe oryzae. Frontiers in Microbiology, 13: 1-12. DOI:10.3389/fmicb.2022.843838
Hussin, N.A., Yeo, F.K.S., Mohamad, N.K., Hamsein, N.N., Thanh, T.A.V., Chung, H.H. & Lai, L.S. (2020). Isolation and characterization of Pyricularia oryzae isolated from lowland rice in Sarawak, Malaysian borneo. Malaysian Journal of Microbiology, 16(1): 58-67. DOI:10.21161/mjm.190423
International Rice Research Institute (IRRI). (n.d.). Rice and the Environment. http://www.knowledgebank.irri.org/ewatermgt/courses/course1/resources/presentations/RiceEnvironment.pdf. Downloaded on 29 April 2023.
Kulkarni, K. & Peshwe, S. (2019). Comparative study of growth and sporulation of Magnaporthe oryzae using different media formulations. International Journal of Scientific & Technology Research, 8(11): 1740-1744.
Kulmitra, A.K., Sahu, N., Sahu, M.K., Kumar, R., Kushram, T. & Kumar, V.B.S. (2017). Growth of rice blast fungus Pyricularia oryzae (Cav.) on different solid and liquid media. International Journal of Current Microbiology and Applied Sciences, 6(6): 1154-1160. DOI:10.20546 /ijcmas.2017.606.133
Kumar, D., Singh, K.P. & Jaiswai, R.K. (2005). Screening of different media and substrates for cultural variability and mass culture of Arthrobotrys dactyloides Drechsler. Mycobiology, 33(4): 215-222. DOI: 10.4489/MY CO.2005.33.4.215
Lai, L.S. (2016). Blast disease and the screening on selected rice varieties. Proceeding Technical Session, Senior Officers’ Conference, 25-27 Oct. 2016.
Lai, L.S. & Eng, L. (2011). Prevalence and severity of paddy blast disease in Sarawak 2009-2010. Research Officers’ Progress Meeting, 4-6 October 2011.
Lai, L.S. & Eng, L. (2013). Prevalence and incidence of paddy blast disease in Sarawak in 2012. Research Officers’ Conference, 1-3 October 2013.
Li, Y., Uddin, W. & Kaminski, J.E. (2014). Effects of relative humidity on infection, colonisation and conidiation of Magnaporthe oryzae on perennial ryegrass. Plant Pathology, 63: 590-597. DOI: 10.1111/ppa.12127
Lujan, P., Sanogo, S., Puppala, N. & Randall, J. (2016). Factors affecting mycelium pigmentation and pathogenicity of Sclerotinia sclerotiorum on Valencia peanut. Canadian Journal of Plant Science, 96(3): 461-473. DOI:10.1139/cjps-2015-0258
Manjunatha, B. & Krishnappa, M. (2019). Morphological characterization of Pyricularia oryzae causing blast disease in rice (Oryza sativa L.) from different zones of Karnataka. Journal of Pharmacognosy and Phytochemistry, 8(3): 3749-3753.
Mohammadpourlima, M., Yassoralipour, A., Tong, P.E., Ahmad, Z.A.M. & Yun, W.M. (2017). Morphological and molecular characterisation of rice blast fungus, Magnaporthe oryzae. Pakistan Journal of Agricultural Sciences, 54(4): 785-792. DOI: 10.21162/PAKJAS/17.3786
Mohsen, L., Al-Janabi, J.K. & Jebor, M.A. (2016). The effect of some environmental conditions on the growth and activity of the external enzymes for five sp. of Fusarium. Journal of Babylon University/Pure and Applied Sciences, 3: 1-17.
Morgulis, A., Coulouris, G., Raytselis, Y., Madden, T.L., Agarwala, R. & Schäffer, A.A. (2008). Database indexing for production MegaBLAST searches. Bioinformatics, 24(16): 1757-1764. DOI: 10.1093/bioinformatics/btn322
Motoyama, T. (2020). Secondary metabolites of the rice blast fungus Pyricularia oryzae: Biosynthesis and biological function. International Journal of Molecular Sciences, 21(22): 1-16. DOI: 10.3390/ijms21228698
Oh, Y., Robertson, S.L., Parker, J., Muddiman, D.C. & Dean, R.A. (2017). Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae. Proteome Science, 15(20): 1-12. DOI:10.11 86/s12953-017-0128-y
Ou, S.H. (1985). Rice Diseases (2nd ed.). Wallingford, UK: C.A.B International.
Pandey, S. (2014). In vitro studies of various culture media, pH, carbon and nitrogen sources on growth of Magnaporthe oryzae causing rice blast. Indian Journal of Tropical Biodiversity, 22(2): 194-198.
Rajput, L.S., Sharma, T., Madhusudhan, P. & Sinha, P. (2017). Effect of temperature on growth and sporulation of rice leaf blast pathogen Magnaporthe oryzae. International Journal of Current Microbiology and Applied Sciences, 6(3): 394-401. DOI: 10.20546/ijcmas.2017.603.045
Skamnioti, P. & Gurr, S.J. (2009). Against the grain: safeguarding rice from rice blast disease. In Trends in Biotechnology, 27(3): 141-150. DOI: 10.1016/j.tibtech.2008.12.002
Srivastava, D., Shamim, M.D., Kumar, D., Pandey, P., Khan, N.A. & Singh, K.N. (2014). Morphological and molecular characterization of Pyricularia oryzae causing blast disease in rice (Oryza sativa) from North India. International Journal of Scientific and Research Publications, 4(7): 2250-3153.
Suzalina, H. (2017). 60,000 tan padi terdedah serangan karah. Berita Harian, 72. Retrieved July 15, 2022, from https://www.pressreader. com/malaysia/berita-harian-malaysia/20170220/ 282527248198351.
TeBeest, D.O., Guerber C. & Ditmore M. (2007). Rice blast. The American Phytopathological Society (APS). Retrieved July 15, 2022, from https://www.apsnet.org/edcenter/disandpath/fungalasco/pdlessons/Pages/RiceBlast.aspx.
Vanaraj, P., Kandasamy, S., Ambalavanan, S., Ramalingam, R. & Sabariyappan, R. (2013). Variability in Pyricularia oryzae from different rice growing regions of Tamil Nadu, India. African Journal of Microbiology Research, 7(26): 3379-3388. DOI: 10.5897/ajmr12.1286
Wang, X., Lee, S., Wang, J., Ma, J., Bianco, T. & Jia, Y. (2014). Current advances on genetic resistance to rice blast disease. In Yan, W. and Bao, J. (eds.) Rice - Germplasm. Genetics and Improvement, Rijeka, Croatia, InTech. pp.195-217. DOI: 10.5772/56824
White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1): 315-322.
Yusop, K., Aziz, A.S.A., Saili, A.R., Nor, R.C.M. & Sahmat, S.S. (2021). Constraints of paddy production among smallholding farmers. International Journal of Service Management and Sustainability, 6(1): 107-119. DOI:10.24191/ ijsms.v6i1.12881.
Zhou, J.M. (2016). Plant pathology: A life and death struggle in rice blast disease. Current Biology, 26(18): 843-845. DOI: 10.1016/ j.cub.2016.08.038
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