Contamination of Bacillus cereus in Foods Sold at Ramadan Bazaar in Kuching, Sarawak
Bacillus cereus in foods at Ramadan bazaar
Every year there are reports of food poisoning involving food sold at the Ramadan bazaar and one of the causes of food poisoning is Bacillus cereus. This study aimed to determine the contamination level of B. cereus and the antimicrobial susceptibility profile of the isolates in food sold at Ramadan bazaar in Kuching, Sarawak. A total of 155 samples from five food categories were collected from six Ramadan bazaars in three locations around Kuching. Overall, 32 (21%) food samples were found positive for B. cereus. Cooked rice showed the least satisfactory levels of B. cereus among the food categories and nasi lemak was the type of food that highly contaminated with B. cereus. Antibiotic susceptibility assessment showed that B. cereus isolates were highly resistant to ampicillin, amoxicillin/clavulanic, penicillin and cefotaxime in this study. The findings of this study showed that B. cereus has the potential to cause food poisoning to Ramadan bazaar consumers due to the high mean count in some types of food. Therefore, this study can be used as basic data for B. cereus control measures in food sold at the Ramadan bazaar.
Bakar, B. (2022). 93 kes keracunan makanan sejak 1 Ramadan. Berita Harian, https://www.bharian. com.my/berita/nasional/2022/04/945787/93-kes-keracunan-makanan-sejak-1-ramadan. Downloaded on 14 April 2022.
Bilung, L.M., Tesfamariam, F., Andriesse, R., San, Y.K.F., Ling, C.Y. & Tahar, A.S. (2018a). Presence of Bacillus cereus from local unhusked (rough) rice samples in Sarawak, Malaysia. Journal of Sustainability Science and Management, 13: 181-187.
Bilung, L.M., Jaraeel, J., Nolasco-Hipólito, C. & Vincent, M. (2018b). Detection, genetic diversity and antibiotic resistance profiles of Bacillus cereus isolated from sago processing plants in Malaysia. Malaysian Journal of Microbiology, 14(4): 320-324.
Clinical and Laboratory Standards Institute (CLSI) (2020). Performance Standards for Antimicrobial Susceptibility Testing. M100. 30th Edition.
Dietrich, R., Jessberger, N., Ehling-Schulz, M., Märtlbauer, M. & Granum, P.E. (2021). The food poisoning toxins of Bacillus cereus. Toxins, 13(98): 1-48.
Elise, N., Mohamed, C., Souleymane, B. & Marianne, S. (2019). A review on Bacillus cereus in Africa’s locally produce foods. European Scientific Journal, 15(9): 228-236.
European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2020). Breakpoint tables for interpretation of MICs and zone diameters. Staphylococcus spp. https://www.eucast.org/file admin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_10.0_Breakpoint_Tables.pdf Downloaded on 15 June 2022.
Fiedler, G., Schneider, C., Igbinosa, E.O., Kabisch, J., Brinks, E., Becker, B., Stoll, D.A., Cho., G., Huch, M. & Franz, C.M.A.P. (2019). Antibiotics resistance and toxin profiles of Bacillus cereus-group isolates from fresh vegetables from German retails markets. BMC Microbiology, 19(250): 1-13.
Fogele, B., Granta, R., Valcin¸ O. & Berzin, A. (2017). Occurrence and diversity of Bacillus cereus and moulds in spices and herbs. Food Control, 83: 69-74
Food Standards Australia New Zealand (FSAND) (2022). Compendium of Microbiological Criteria for Food. https://www.foodstandards.gov.au/pub lications/Documents/Compendium_revised% 20 March%202022.pdf Downloaded on 10 June 2022.
Gao, T., Ding, T., Wu, Q., Wang, J., Zhang J., Yu, S., Yu, P., Liu, C., Kong, L., Feng, Z., Chen, M., Wu, S., Zeng, H. & Wu, H. (2018). Prevalence, virulence genes, antimicrobial susceptibility and genetic diversity of Bacillus cereus isolated from pasteurized milk in China. Frontiers in Microbiology, 9(553): 1-11.
Gdoura-Ben Amor, M., Siala, M., Zayani, M., Grosset, N., Smaoui, S., Messadi-Akrout, F., Barom, F., Jan, S., Gautier, M. & Gdoura, R. (2018). Isolation, identification, prevalence and genetic diversity of Bacillus cereus group bacteria from different foodstuffs in Tunisia. Frontiers in Microbiology, 9(447): 1-12.
Idris, S.R. (2022). 57 notis kompaun dikenakan kepada peniaga sepanjang Ramadan. Berita Harian. https://www.bharian.com.my/berita/wila yah/2022/04/946919/57-notis-kompaun-dikenak an-kepada-peniaga-sepanjang-ramadan. Downloaded on 17 April 2022.
Ikeda, M., Yagihara, Y., Tatsuno, K., Okazaki, M., Okugawa, S. & Moriya, K. (2015). Clinical characteristics and antimicrobial susceptibility of Bacillus cereus blood stream infections. Annals of Clinical Micrbiology and Antimicrobials, 14(43): 1-7.
ISO 7932 (2004). Microbiology of the food and animal feeding stuffs - Horizontal method for the eunemaration of Bacillus cereus- Colony-count technique at 30 °C. Geneva: International Organisation for Standardization. https://www.iso.org/standard/38219.html. Downloaded on 12 Jan 2022.
Jamaluddin, N.A.H., Periyasamy, P., Lau, C.L., Ponnampalavanar, S., Lai, P.S.M., Ramli, R., Tan, T.L., Kori, N., Yin, M.K., Azman, N.J., James, R., Thursky, K. & Naina-Mohamed, I. (2021). Point prevalence survey of antimicrobial use in a Malaysian tertiary care university hospital. Antibiotics, 10(531): 1-13.
Khadka, S., Adhikari, S., Rai, T., Ghimire, U. & Parajuli, A. (2018). Bacterial contamination and risk factors associated with street vended Panipuri sold in Bharatpur, Nepal. International Journal of Food Research, 5: 32-38.
Khasnabis, J., Adhikari, P., Chowdhury, D., Rai, C. & Roy, A. (2017). Incidence of multiple drug resistant Bacillus cereus in some popular snacks and sweets sold in Kolkata city, India. Indian Journal Microbiol Research, 4(1):14-19.
Mohd Asmawi, U.M., Norehan, A.A., Salikin, K., Rosdi, N.A.S., Abdul Munir, N.A.T., Muhammad Basri, N.B., Selamat, M.I. & Md Nor, N. (2018). An assessment of knowledge, attitudes and practices in food safety among food handlers engaged in food courts. Current Research in Nutrition and Food Science, 6(2): 346-353.
Msarah, M., Alsier, A. & Sahilah, A.M. (2020). Detection of HBLD toxin gene by Bacillus cereus isolated from meat curry food samples in Malaysian restaurants. Environment & Ecosystem Science, 4(2): 51-55.
Navaneethan, Y. & Effarizah, M.E. (2021). Prevalence, toxigenic profiles, multidrug resistance, and biofilm formation of Bacillus cereus isolated from ready-to eat cooked rice in Penang, Malaysia. Food Control, 121(2021): 107553.
Park, K.M., Kim, H.J., Jeong, M. & Koo, M. (2020). Enterotoxin genes, antibiotic susceptibility, and biofilm formation of low-temperature-tolerant Bacillus cereus isolated from green leaf lettuce in the cold chain. Foods, 9: 249.
Rusnan, A.N., Nordin, N., Radu, S. & Abdul-Mutalib, N.A. (2020). Pathogenic Bacillus cereus, an overlooked food contaminant in Southeast Asia. Pertanika Journal of Tropical Agricultural Science, 43(1): 1-17.
Saba, C.K.S., Antwi, M.V. & Adzitey, F. (2019). Prevalence of Bacillus cereus in ready-to-eat boiled and fried rice in the Tamale Metropolis of Ghana. Journal of Food Safety and Hygiene, 5(1): 20-23.
Tewari, A. & Abdullah, S. (2015). Bacillus cereus food poisoning: International and Indian perspective. Journal of Food Science and Technology, 52(5): 2500–2511.
Ubong, A., New, C.Y., Chai, L.C., Loo, Y.Y., Nor Khaizura, M.A.R., Kayali, A.Y. & Son, R. (2020). Prevalence of Bacillus cereus in ultra-high temperature chocolate milk from selected milk manufacturers in Malaysia. Food Research, 4(4): 982-990.
Vedesh, R. & Neel, A.C. (2017). Microbial analysis of street foods of different locations at Chennai City, India. Innovate International Journal of Medical & Pharmaceutical Sciences, 2(1): 21-23.
Yu, S., Yu, P., Wang, J., Li, C., Guo, H., Liu, C., Kong, Li, Yu, L., Wu, S., Lei, T., Chen, M., Zeng, H., Pang, R., Zhang, Y., Wei, X., Zhang, J., Wu, Q. & Ding, Y. (2020). A study on prevalence and characterization of Bacillus cereus in ready-to-eat foods in China. Frontiers in Microbiology, 10(3043): 1-11.
Zulfakar, S.S., Zin, N.M., Zalami, S.N.S.M. & Nawawee, N.S.M. (2021). Identification and antibiotic resistance profile of Salmonella spp. and Citrobacter spp. isolated from street-vended beverages. Pertanika Journal of Science and Technology, 29(1): 593-606.
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