Histopathology of Pre-diabetic White Rat (Rattus norvegicus L.) Renal After Treatment with Turmeric Powder and Organic Quail Eggs
DOI:
https://doi.org/10.33736/bjrst.4920.2022Keywords:
Kidney tissue, organic quail eggs, pre-diabetic, turmeric powderAbstract
Pre-diabetic is a disease that is caused by insulin resistance, which is identified by higher blood glucose levels than normal. Turmeric (Curcuma longa L.) contains curcumin compounds that act as antioxidants to prevent damage from free radicals and to repair damaged kidney tissue from pre-diabetic condition. Organic quail eggs are supplements that can help to repair kidney tissue. This study investigated the effect of turmeric powder and organic quail eggs on pre-diabetic kidney tissue histopathology of male white rats. Twenty-five male white rats (Rattus norvegicus L.) were used in this study and they were divided into 5 treatment groups, namely D0 (normal white rats were given standard diet), D1 (positive control, pre-diabetic white rats were given standard diet), D2 (pre-diabetic white rats were given turmeric powder 1.35 mg/head/day), D3 (pre-diabetic white rats were given 1 organic quail egg/head/day), and D4 (pre-diabetic white rats were given turmeric powder 1.35 mg/head/day and 1 organic quail egg/head/day). This research was done within 60 days. The results of the study were analysed using the ANOVA and Duncan tests. The analysis results showed that turmeric powder and organic quail eggs treatments had a significant effect on the observed parameters. The conclusion of this study is that the effect of turmeric powder and organic quail eggs has the potential to repair the kidney tissue of pre-diabetic white rats
References
Aba, P.E., Igwebuike, D.C. & Onah, J.A. (2015). Effects of various concentrations of quail egg solution on glycemia and antioxidant parameters of alloxan-induced diabetic rats. Journal of Advances in Medical and Pharmaceutical Sciences, 5(4): 1-7.
https://doi.org/10.9734/JAMPS/2016/22723
Al-Malki, A.L. & El-Rabey, H.A. (2015). The antidiabetic effect of low doses of Moringa oleifera Lam. seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats. BioMed Research International, 2015: 1-13.
https://doi.org/10.1155/2015/381040
Ando, M., Magi, S., Seki, M., Suzuki, Y., Kasukawa, T., Lefaudeux, D., Hoffmann, A. & Okada, M. (2021). IκBα is required for full transcriptional induction of some NFκB-regulated genes in response to TNF in MCF-7 Cells. Nature Partner Journal Systems Biology and Applications, 7(1): 1-15.
https://doi.org/10.1038/s41540-021-00204-7
Andrie, M., Taurina, W. & Ayunda, R.. (2014). Activities test of "Jamu Gendong Kunyit Asam" (Curcuma domestica Val.; Tamarindus indica L.) as an antidiabetic in streptozotocin-induced rats. Majalah Obat Tradisional, 19(2): 95-102.
Arief, I.I., Jenie, B.S.L., Astawan, M. & Witarto, A.B. (2010). Efektivitas probiotik Lactobacillus plantarum 2C12 dan Lactobacillus acidophilus 2B4 sebagai pencegah diare pada tikus percobaan. Media Peternakan, 33(3): 137-137.
https://doi.org/10.5398/medpet.2010.33.3.137
Azhari, B., Luliana, S. & Robiyanto (2017). Antihypercholestrolemic activity of aqueous extract of belimbing wuluh (Averrhoa bilimbi Linn.) on hypercholesterolemic modelling wistar male rats. Majalah Obat Tradisional, 22(1): 57-62.
https://doi.org/10.22146/tradmedj.24319
Basri, H. (2018). Status leukosit dan differential leukosit tikus putih (Rattus norvegicus L.) periode laktasi setelah diberi suplement telur puyuh organik. Biota: Biologi dan Pendidikan Biologi, 11(2): 136-145.
https://doi.org/10.20414/jb.v11i2.112
Celsi, G., Larsson, L., Seri, L., Savin, V. & Aperia, A.. (1989). Glomerular adaptation in uninephrectomized young rats. Pediatric Nephrology, 3(3): 280-285.
https://doi.org/10.1007/BF00858530
Chen, J., Mangelinckx, S., Adams, A., Wang, Z.T., Li, W.L., & De Kimpe, N. (2015). Natural flavonoids as potential herbal medication for the treatment of diabetes mellitus and its complications. Natural Product Communications, 10(1): 187-200.
https://doi.org/10.1177/1934578X1501000140
Dharmaputra, G.A., EVeronica, E., Driansha, C.L., Satyarsa, A.B.S. & Dewi, N.W.S. (2020). Potensi ekstrak kunyit (Curcuma longa) terhadap kadar ischemia modified albumin (ima) pada tikus model penyakit jantung koroner. Jurnal Kesehatan Andalas, 9(3): 328-333.
https://doi.org/10.25077/jka.v9i3.1392
Efendi, E., Prasetyo, F.A., Taufiqurrahman, M., Ariesaka, K.M., Insani, A.Y. & Ariansari, B.G. (2016). Efek nefroprotektif ekstrak bawang kucai (Allium tuberosum) terhadap kadar blood urea nitrogen (bun) dan kreatinin tikus wistar yang diinduksi doxorubicin. NurseLine Journal, 1(2): 203-211.
El-Masry, A.A. (2012). Potential therapeutic effect of Curcuma longa on streptozotocin induced diabetic rats. Global Advanced Research Journal of Medicine and Medical Sciences, 1(4): 91-98.
Fahrimal, Y., Rahmiwati & Dwinna, A. (2016). Gambaran histopatologis ginjal tikus putih (Rattus novergicus) jantan yang diinfeksikan Trypanosoma evansi dan diberi ekstrak daun sernai (Wedelia biflora). Jurnal Medika Veterinaria, 10(2): 166-170.
https://doi.org/10.21157/j.med.vet..v10i2.4386
Fahriyansyah, F., Isdadiyanto, S., Mardiati, S.M. & Sitasiwi, A.J. (2021). Gambaran histologi ren tikus putih (Rattus norvegicus L.) hiperglikemia setelah pemberian ekstrak. Buletin Anatomi dan Fisiologi, 6(2): 193-202.
https://doi.org/10.14710/baf.6.2.2021.193-202
Franco-Robles, E., Campos-Cervantes, A., Murillo-Ortiz, B.O., Segovia, J., López-Briones, S., Vergara, P., Pérez-Vázquez, P., Solís-Ortiz, M.S. & Ramírez-Emiliano, J. (2014). Effects of curcumin on brain-derived neurotrophic factor levels and oxidative damage in obesity and diabetes. Applied Physiology, Nutrition, and Metabolism, 39(2): 211-218.
https://doi.org/10.1139/apnm-2013-0133
Genchev, A. (2012). Quality and composition of japanese quail eggs (Coturnix japonica). Trakia Journal of Sciences 10(2): 91-101.
Gómez, J.C. & Lorido, J.A. (2018). Clinical assessment and treatment of diabetes in patients with chronic kidney disease. Revista Clínica Española (English Edition), 218(6): 305-315.
https://doi.org/10.1016/j.rceng.2018.03.010
Gultom, S.M. & Supratman, H. (2012). Pengaruh imbangan energi dan protein ransum terhadap bobot karkas dan bobot lemak abdominal ayam broiler umur 3-5 minggu. Students e-Journal, 1(1): 1-15
Gumolung, D. (2017). Analisis beta karoten dari ekstrak jonjot buah labu kuning (Cucurbita moschata). Fullerene Journal of Chemistry, 2(2): 69-71.
https://doi.org/10.37033/fjc.v2i2.12
Hidayat, R. (2015). Peroxisome Proliferator Activator Receptor (PPAR) γ agonis menurunkan kadar sitokin anti inflamasi TGF-β dan IL-10 pada tikus putih wistar model inflamasi vaskular. Jurnal Kedokteran dan Kesehatan, 2(3): 291-296.
Isdadiyanto, S. (2018). Tebal dinding dan diameter lumen arteria koronaria tikus putih setelah pemberian teh kombucha kadar 100% waktu fermentasi 6, 9 dan 12 hari. Buletin Anatomi Dan Fisiologi, 3(1): 97.
https://doi.org/10.14710/baf.3.1.2018.97-104
Jacob, A., Chaves, L., Eadon, M.T., Chang, A., Quigg, R.J. & Alexander, J.J. (2013). Curcumin alleviates immune‐complex‐mediated glomerulonephritis in factor‐H‐deficient mice. Immunology, 139(3): 328-337.
https://doi.org/10.1111/imm.12079
Jusuf, A.A. (2009). Histoteknik Dasar. Jakarta: Fakultas Kedokteran Universitas Indonesia.
Khalishah, H., Kurniawan, H., Nugraha, F., Nurbaeti, S.N. & Fajriaty, I. (2021). Pengaruh pemberian serbuk cangkang telur terhadap bobot badan dan indeks organ tikus putih (Rattus norvegicus l.) galur wistar dalam 28 hari. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 5(1): 1-6.
Kotyk, T.N., Dey, A.S., Ashour, D., Balas, T.S., Chakraborty, A.S., Ashour & Tavares, J.M.R.S. (2016). Measurement of glomerulus diameter and Bowman's space width of renal albino rats. Computer Methods and Programs in Biomedicine, 126: 143-153.
https://doi.org/10.1016/j.cmpb.2015.10.023
Kuncarli, I. & Djunarko, I. (2014). Uji toksisitas subkronis infusa daun sirih merah (Piper crocatum ruiz & pav) pada tikus: studi terhadap gambaran mikroskopis jantung dan kadar sgot darah. Jurnal Farmasi Sains dan Komunitas. Journal of Pharmaceutical Sciences and Community, 11(2): 86-95.
Lismawati, L., Tutik, T. & Nofita, N. (2021). Kandungan beta karoten dan aktivitas antioksidan terhadap ekstrak buah labu kuning (Cucurbita moschata). Jurnal Mandala Pharmacon Indonesia, 7(2): 263-273.
Luc, K., Schramm-Luc, A., Guzik, T.J. & Mikolajczyk, T.P. (2019). Oxidative stress and inflammatory markers in prediabetes and diabetes. Journal Physiology Pharmacology, 70(6): 809-824.
Malik, M., Ulma, A.B. Sarmoko & Nugraha, Y. (2021). Fungsi kurkumin sebagai antidiabetes pada tingkat molekular. Acta Pharmaciae Indonesia, 9(1): 70-77.
https://doi.org/10.20884/1.api.2021.9.1.3323
Mappa, I.S., Kairupan, C. & Loho, L. (2013). Gambaran hitologi ginjal tikus putih (Wistar) setelah pemberian rifampisin. e-Biomedik, 1(1): 338-342.
https://doi.org/10.35790/ebm.1.1.2013.4368
Maslachah, L.R., Sugihartuti & Kurniasanti, R. (2008). Hambatan produksi reactive oxygen species radikal superoksida (O2-) oleh antioksidan vitamin E (α-tocopherol) pada tikus putih (Rattus norvegicus) yang menerima stressor renjatan listrik. Media Kedokteran Hewan, 24(1): 21-26.
Mayori, R., Marusin, N. & Tjong, D.H. (2013). Pengaruh pemberian rhodamin b terhadap struktur histologis ginjal mencit putih (Mus musculus L.). Jurnal Biologi Unand, 2(1): 43-49.
Mediani, A., Abas, F. Maulidiani, M., Khatib, A., Tan, C.P., Ismail, I.S. Shaari, K., Ismail, A. & Lajis, N.H. (2016). Metabolic and biochemical changes in streptozotocin induced obese-diabetic rats treated with Phyllanthus niruri extract. Journal of Pharmaceutical and Biomedical Analysis, 128: 302-312.
https://doi.org/10.1016/j.jpba.2016.06.003
Muliani, H. (2010). Struktur mikroanatomi ren ayam (Gallus sp.) karena pengaruh pemberian kunyit putih (Curcuma zedoaria). Anatomi Fisiologi, 18(2): 8-20.
Nugroho, A.D., Nilasari, K., Putri, V.A., Sumekar, T.A., Karlowee, V. Hardian & Gumay, A.R. (2019). Turmeric as a preventive agent of oxidative stress and diabetic nephropathy in alloxan induced wistar rats. Pakistan Journal of Medical and Health Sciences, 13(4): 1208-1213.
Nugroho, S. (2012). Pencegahan dan pengendalian diabetes melitus melalui olahraga. Medikora, 9(1): 1-15.
Piwkowska, A., Rogacka, D., Kasztan, M., Angielski, S. & Jankowski, M. (2013). Insulin increases glomerular filtration barrier permeability through dimerization of protein kinase G type Iα subunits. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1832(6): 791-804.
https://doi.org/10.1016/j.bbadis.2013.02.011
Pivari, F., Mingione, A. Brasacchio, C. & Soldati, L. (2019). Curcumin and type 2 diabetes mellitus. Prevention and Treatment. Nutrients, 1(8): 1-12.
https://doi.org/10.3390/nu11081837
Rabiah, E.S., Berata, K. & Samsuri (2015). Gambaran histopatologi ginjal tikus putih yang diberi deksametason dan vitamin E. Indonesia Medicus Veterinus, 4(1): 249-256.
Rajak, Z.F., Loho, L. & Lintong, P. (2016). Gambaran histopatologik ginjal wistar yang diberi ekstrak binahong pasca pemberian gentamisin. eBiomedik, 4(2): 1-6.
https://doi.org/10.35790/ebm.4.2.2016.13911
Ratnasari, A.A., Winarto, H. Purbadi, S., Sri M, Sekarutami & Sutrisna, B. (2016). Hubungan ekspresi NFκB dengan respons radiasi kanker serviks stadium lokal lanjut. eJournal Kedokteran Indonesia, 4(1): 31-36.
https://doi.org/10.23886/ejki.4.5906.31-6
Riandika, C., Saraswati, T.R. & Isdadiyanto, S. (2019). Kadar glukosa darah tikus putih (Rattus norvegicus L.) pada periode laktasi setelah pemberian suplemen telur puyuh organik. Buletin Anatomi dan Fisiologi, 4(2): 122-128.
https://doi.org/10.14710/baf.4.2.2019.122-128
Rohman, J.H.F., Sunarno, Isdadiyanto, S. & Mardiati, S.M. (2021). Efek minuman berenergi terhadap histopatologis ginjal tikus putih (Rattus norvegicus). Media Bina Ilmiah, 15(7): 4835-4848.
Saraswati, T.R. & Isdadiyanto, S. (2018). The effect of organic quail egg supplementation on the blood lipid profile of white mice (Rattus Norvegicus L.) during the lactation period. Journal of Physics: Conference Series, 1025(1): 1-8.
https://doi.org/10.1088/1742-6596/1025/1/012077
Saraswati, T.R. & Tana, S. (2016). Effect of turmeric powder supplementation to the age of sexual maturity, physical, and chemical quality of the first japanese quail's (Coturnix japonica) egg. Journal of Biology & Biology Education, 8(1): 18-24.
https://doi.org/10.15294/biosaintifika.v8i1.4982
Saraswati, T.R., Suartini, G.A.A. & Tana, S. (2021). Development of diabetic animal model via insulin glargine. Asian Journal of Animal Sciences, 15(2): 67-74.
https://doi.org/10.3923/ajas.2021.67.74
Schrijvers, B.F., Vriese, A.S.D. & Flyvbjerg, A. (2004). From hyperglycemia to diabetic kidney disease: the role of metabolic, hemodynamic, intracellular factors and growth factors/cytokines. Endocrine Reviews, 25(6): 971-1010.
https://doi.org/10.1210/er.2003-0018
Septiva, E.B., Sitasiwi, A.J. & Isdadiyanto, S. (2019). Struktur mikroanatomi ginjal mencit (Mus musculus L.) betina setelah paparan ekstrak etanol daun mimba (Azadirachta indica A. Juss). Jurnal Pro-Life, 6(2): 180-190.
https://doi.org/10.33541/pro-life.v6i1.931
Simanjuntak, P. (2015). Studi kimia dan farmakologi tanaman kunyit (Curcuma longa L) sebagai tumbuhan obat serbaguna. Jurnal Ilmu Pertanian, 17(2): 103-107.
Singh, D.K. & Farrington, K. (2010). The tubulointerstitium in early diabetic nephropathy: prime target or bystander?. International Journal of Diabetes in Developing Countries, 30 (4): 185-190.
https://doi.org/10.4103/0973-3930.70857
Sovia, E., Sukandar, E.Y., Sigit, J.I. & Sasongko, L.D.N. (2011). Efek rimpang kunyit (Curcuma longa L.) dan bawang putih (Allium sativum L.) terhadap sensitivitas insulin pada tikus galur wistar. Majalah Kedokteran Bandung, 43(4): 153-159.
https://doi.org/10.15395/mkb.v43n4.62
Sudargo, T., Prameswari, A.A., Aulia, B., Aristasari, T., Alfionita, K., Muslichah, R., Isnansetyo, A., Puspita, I.D., Budhiyanti, S.A. & Putri, S.R. (2021). Analisis sensoris dan umur simpan makanan selingan prediabetes berbasis tuna (Thunnus sp.) dan labu siam (Sechium edule). Media Gizi Mikro Indonesia, 12(2): 153-164.
https://doi.org/10.22435/mgmi.v12i2.4952
Syam, I.A., Hatta, R. & Ruslin, M. (2015). Potensi dari ceker ayam kampung (Gallus domesticus) untuk mempercepat penyembuhan soket pascaekstraksi gigi. Makassar Dental Journal, 4(2): 50-55.
Tandi, J., Wulandari, A. & Asrifa (2017). Efek ekstrak etanol daun gendola merah (Basella alba L.) terhadap kadar kreatinin, ureum dan deskripsi histologis tubulus ginjal tikus putih jantan (Rattus norvegicus) diabetes yang diinduksi streptozotocin. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (E-Journal), 3(2): 93-102.
https://doi.org/10.22487/j24428744.0.v0.i0.8813
Teușan, A., Vacaru-Opriș, I. & Teușan, V. (2008). Aspects regarding some morphological values of the domestic quail eggs (Coturnix coturnix japonica). Lucrări ştiinŃifice Zootehnie şi Biotehnologii, 41(2): 709-716.
Vallon, V. (2011). The proximal tubule in the pathophysiology of the diabetic kidney. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 300(5): 1009-1022.
https://doi.org/10.1152/ajpregu.00809.2010
Vessal, M., Hemmati, M. & Vasei, M. (2003). Antidiabetic effects of quercetin in streptozocin-induced diabetic rats. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 135(3): 357-364.
https://doi.org/10.1016/S1532-0456(03)00140-6
Wahyuni, E., Sri & Hermawati (2017). Persepsi pemenuhan kebutuhan nutrisi pada pasien diabetes mellitus di desa sawah kuwung karang anyar. Jurnal Care, 5(2): 306-317.
https://doi.org/10.33366/cr.v5i2.571
Walton, S.L., Moritz, K.M., Bertram, J.F. & Singh, R.R. (2016). Lengths of nephron tubule segments and collecting ducts in the CD-1 mouse kidney: an ontogeny study. American Journal of Physiology-Renal Physiology, 311(5): 976-983.
https://doi.org/10.1152/ajprenal.00435.2016
Wibawa, I.M.B.C., Putra, A.I.Y.D. & Tandiono, E.K. (2019). Potensi curcumin dalam ekstrak rimpang kunyit (Curcumma domestica L.). Essence of Scientific Medical Journal, 38-42.
Widiastuti, E.L., Ardiansyah, B.K., Nurcahyani, N. & Silvinia, A. (2021). Antidiabetic potency of jeruju (Acanthus ilicifolius L.) ethanol extract and taurine on histopathological response of mice kidney (Mus musculus L.) induced by alloxan. Journal of Physics, 1751(1): 1-9.
https://doi.org/10.1088/1742-6596/1751/1/012052
Wiladipta, D., Pramono, A. & Purnomo, Y. (2021). Potensi sari biji kedelai (Glycine max), sari rimpang jahe (Zingiber officinale) dan kombinasinya terhadap kadar malondialdehida (MDA) dan diameter glomerulus ginjal pada tikus model diabetes tipe II. Jurnal Kedokteran Komunitas, 9(1): 1-9.
Yang, X., Mei, S., Gu, H., Guo, H., Zha, L., Cai, J., Li, X., Liu, Z. & Cao, W. (2014). Exposure to excess insulin (Glargine) induces Type 2 Diabetes Mellitus in mice fed on a chow diet. The Journal of Endocrinology, 221(3): 469-480.
https://doi.org/10.1530/JOE-14-0117
Yimagou, L.E., Tanya, A., Tchankou, C., Ngondi, J. & Oben, J. (2016). Metabolic effects of quail eggs in diabetes-induced rats: comparison with chicken eggs. Food and Nutrition Research, 60(1): 1-7.
https://doi.org/10.3402/fnr.v60.32530
Zhang, S., Xu, L., Liang, R., Yang, C. & Wang, P. (2020). Baicalin suppresses renal fibrosis through microrna-124/TLR4/NF-Κb axis in streptozotocin -induced diabetic nephropathy mice and high glucose-treated human proximal tubule epithelial cells. Journal of Physiology and Biochemistry, 76(3): 407-416.
https://doi.org/10.1007/s13105-020-00747-z
Zulfadhli, N., Wijayanti, B. & Retnoaji (2016). Perkembangan ovarium ikan wader pari (Rasbora lateristriata) pendekatan histologi. Jurnal Perikanan Tropis, 3(1): 32-38.
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