Effect of Thermal Treatment on Kelulut Honey Towards the Physicochemical, Antioxidant and Antimicrobial Properties
DOI:
https://doi.org/10.33736/bjrst.4645.2022Keywords:
Antibacterial, antioxidant, stingless bee honey, thermal treatmentAbstract
Heat treatment on commercial honey could deteriorate its quality associated with physicochemical and bioactive capacities. In this study, the effects of thermal treatment (50 °C, 75 °C and 90 °C) on the physicochemical properties (i.e., pH, colour intensity), total phenolic content and total flavonoid content were investigated on the Kelulut honey. The results revealed a significant increase in TFC (0.154 mg QE/g honey) for the heat-treated Kelulut honey compared to the control (0.085 mg QE/g honey). The antioxidant activity of the heat-treated honey revealed an increase in 2, 2- Diphenyl-1-picrylhydrazyl levels by 42%, while the ferric reducing antioxidant power levels were reduced significantly by 22.4% compared to the untreated honey. The antimicrobial activities of heat-treated honey declined against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Salmonella typhi bacteria at 75 °C and 90 °C. Based on the effects observed in the bioactive capacities of the heat-treated honey, it is therefore recommended to minimize thermal treatment on the honey during the processing to maintain its natural nutritional quality and benefit consumers.
References
Abd Jalil, M.A., Kasmuri, A.R. & Hadi, H. (2017). Stingless bee honey, the natural wound healer: A review. Skin Pharmacology and Physiology, 30: 66-75.
https://doi.org/10.1159/000458416
Akgün, N., Çelik, Ö.F. & Kelebekli, L. (2021). Physicochemical properties, total phenolic content, and antioxidant activity of chestnut, rhododendron, acacia and multifloral honey. Journal of Food Measurement and Characterization, 26: 1-8.
https://doi.org/10.1007/s11694-021-00937-3
Al, M.L., Daniel, D., Moise, A., Bobis, O., Laslo, L. & Bogdanov, S. (2009). Physico-chemical and bioactive properties of different floral origin honeys from Romania. Food Chemistry, 112(4): 863-867.
https://doi.org/10.1016/j.foodchem.2008.06.055
Ali, H., Abu Bakar, M.F., Majid, M., Muhammad, N. & Lim, S.Y. (2020). In vitro antidiabetic activity of stingless bee honey from different botanical origins. Food Research, 4(5): 1421-1426.
https://doi.org/10.26656/fr.2017.4(5).411
Almasaudi, S.B., Al-Nahari, A.A.M., Abd El-Ghany, E.S.M., Barbour, E., Al Muhayawi, S.M., Al-Jaouni, S., Azhar, E., Qari, M., Qari, Y.A. & Harakeh, S. (2017). Antimicrobial effect of different types of honey on Staphylococcus aureus. Saudi Journal of Biological Sciences, 24(6): 1255-1261.
https://doi.org/10.1016/j.sjbs.2016.08.007
Azlim Almey, A.A., Ahmed Jalal Khan, C., Syed Zahir, I., Mustapha Suleiman, K., Aisyah, M.R. & Kamarul Rahim, K. (2010). Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants' leaves. International Food Research Journal, 17(4): 1077-1084.
Boussaid, A., Chouaibi, M., Rezig, L., Hellal, R., Donsì, F., Ferrari, G. & Hamdi, S. (2018). Physicochemical and bioactive properties of six honey samples from various floral origins from Tunisia. Arabian Journal of Chemistry, 11(2): 265-274.
https://doi.org/10.1016/j.arabjc.2014.08.011
Braghini, F., Biluca, F.C., Gonzaga, L.V., Kracik, A.S., Vieira, C.R.W., Vitali, L., Micke, G.A., Costa, A.C.O. & Fett, R. (2019). Impact of short-term thermal treatment on stingless bee honey (Meliponinae): Quality, phenolic compounds and antioxidant capacity. Journal of Food Processing and Preservation, 43(7): 1-8.
https://doi.org/10.1111/jfpp.13954
Carciochi, R.A., Galván, D., Alessandro, L. & Manrique G.D. (2016). Effect of roasting conditions on the antioxidant compounds of quinoa seeds. International Journal of Food Science Technology, 51(4): 1018-1025.
https://doi.org/10.1111/ijfs.13061
Chan, B.K., Haron, H., Talib, R.A. & Subramaniam, P. (2017). Physical properties, antioxidant content and anti-oxidative activities of Malaysian Stingless Kelulut (Trigona spp.) honey. Journal of Agricultural Science, 9(13): 32-40.
https://doi.org/10.5539/jas.v9n13p32
Chen, C., Campbell, L.T., Blair, S.E. & Carter, D.A. (2012). The effect of standard heat and filtration processing procedures on antimicrobial activity and hydrogen peroxide levels in honey. Frontiers in Microbiology, 3: 1-8.
https://doi.org/10.3389/fmicb.2012.00265
Chong, K.Y., Chin, N.L. & Yusof. Y.A. (2017). Thermosonication and optimization of stingless bee honey processing. Food Science and Technology International, 23(7): 608-622.
https://doi.org/10.1177/1082013217713331
Chua, L.S., Adnan, N.A., Abdul-Rahaman, N.L. & Sarmidi, M.R. (2014). Effect of thermal treatment on the biochemical composition of tropical honey samples. International Food Research Journal, 21(2): 773-778.
Cooper, R.A., Jenkins, L., Henriques, A.F., Duggan, R.S. & Burton, N.F. (2010). Absence of bacterial resistance to medical-grade manuka honey. European Journal of Clinical Microbiology, 29(10): 1237-1241.
https://doi.org/10.1007/s10096-010-0992-1
Diva, A.N., Pratami, D.K., Wijanarko, A., Hermansyah, H. & Sahlan, M. (2019). Effect of ethanolic propolis extract from Tetragonula biroi bees on the growth of human cancer cell lines HeLa and MCF-7. AIP Conference Proceedings, 2092(1): 1-6.
https://doi.org/10.1063/1.5096706
Elamine, Y., Anjos, O., Estevinho, L.M., Lyoussi, B., Aazza, S. & Miguel, M.G. (2020). Effect of extreme heat processing on the Moroccan Zantaz' honey antioxidant activities. Journal of Food Science and Technology, 57(9): 3323-3333.
https://doi.org/10.1007/s13197-020-04365-x
Froschle, M., Horn, H. & Spring, O. (2018). Characterization of Jatropha curcas honeys originating from the southern highlands of Madagascar. LWT - Food Science and Technology, 93: 525-533.
https://doi.org/10.1016/j.lwt.2018.04.006
Iqbal, M., Fan, T.P., Watson, D. Alenezi, S., Saleh, K. & Sahlan, M. (2019). Preliminary studies: the potential antiangiogenic activities of two Sulawesi Island (Indonesia) propolis and their chemical characterization. Heliyon, 5(7): 1-12.
https://doi.org/10.1016/j.heliyon.2019.e01978
Ismail, M.M. (2014). Competitiveness of beekeeping industry in Malaysia, Inaugural Lecture Series. Serdang: Universiti Putra Malaysia. Pp. 1-70.
Lindquist E. & Yang Y. (2011). Degradation of benzoic acid and its derivatives in subcritical water. Journal of Chromatography A, 1218(15): 2146-2152.
https://doi.org/10.1016/j.chroma.2010.08.054
Mahnot, N.K., Saikia, S. & Mahanta, C.L. (2019). Quality characterization and effect of sonication time on bioactive properties of honey from North East India. Journal of Food Science and Technology, 56(2): 724-736.
https://doi.org/10.1007/s13197-018-3531-1
Miyata, R., Sahlan, M., Ishikawa, Y., Hashimoto, H., Honda, S. & Kumazawa, S. (2019). Propolis components from stingless bees collected on South Sulawesi, Indonesia, and their xanthine oxidase inhibitory activity. Journal of Natural Products, 82: 205-210.
https://doi.org/10.1021/acs.jnatprod.8b00541
Moniruzzaman, M., An, C., Rao, P., Hawlader, M., Mohd Azlan, S., Sulaiman, S. & Gan, S. (2014). Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: Determination of antioxidant capacity. BioMed Research International, 2014: 1-11.
https://doi.org/10.1155/2014/737490
Moniruzzaman, M., Khalil, I., Sulaiman, S.A. & Gan, S.H. (2013). Physicochemical and antioxidant properties of Malaysian honeys produced by Apis cerana, Apis dorsata and Apis mellifera. BMC Complementary and Alternative Medicine, 13(43): 1-12.
https://doi.org/10.1186/1472-6882-13-43
Nayik, G.A. & Nanda, V. (2016). Effect of thermal treatment and pH on antioxidant activity of saffron honey using response surface methodology. Food Measure, 10(1): 64-70.
https://doi.org/10.1007/s11694-015-9277-9
Ngaini, Z., Hussain, H., Kelabo, E.S., Wahi, R. & Farooq, S. (2021a). Chemical profiling, biological properties and environmental contaminants of stingless bee honey and propolis. Journal of Apicultural Research, 1-17.
https://doi.org/10.1080/00218839.2021.1948745
Ngaini, Z., Kelabo, E.S., Hussain, H. & Wahi, W. (2021b). High therapeutic properties of honey from the Borneo stingless bee, Heterotrigona itama. International Journal of Current Research, 13(4): 100-107.
https://doi.org/10.31782/IJCRR.2021.SP131
Nijveldt, RJ., Van Nood, E.L.S., Van Hoorn, D.E., Boelens, P.G., Van Norren, K. & Van Leeuwen, P.A. (2001). Flavonoids: A review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition, 74(4): 418-425.
https://doi.org/10.1093/ajcn/74.4.418
Onyeka, O., Okeke, M.U., Ezejiofor, C.C. & Ndubuisi, J.O. (2018). Antimicrobial activity of honeys from Nsukka and Ugwuaji in Enugu state, on selected pathogenic bacteria isolated from wound. Advances in Analytical Chemistry, 8(1): 6-9.
Rao, P.V., Krishnan, K.T., Salleh, N. & Gan, S.H. (2016). Biological and therapeutic effects of honey produced by honey bees and stingless bees: A comparative review. Revista Brasileira de Farmacognosia, 26(5): 657-664.
https://doi.org/10.1016/j.bjp.2016.01.012
Romainor, A.N.B., Chin, S.F., Pang, S.C. & Bilung, L.M. (2014). Preparation and characterization of chitosan nanoparticles-doped cellulose films with antimicrobial property. Journal of Nanomaterials, 2014: 1-10.
https://doi.org/10.1155/2014/710459
Sahlan, M., Mahira, K.F., Wiratama, I., Mahadewi, A.G., Yohda, M., Hermansyah, H. & Noguchi, K. (2019). Purification and characterization of proteins in multifloral honey from Kelulut bee (stingless bee). Heliyon, 5: 1-11.
https://doi.org/10.1016/j.heliyon.2019.e02835
Šarić, G., Marković, K., Vukičević, D., Lež, E., Hruškar, M. & Vahčić, N. (2013). Changes of antioxidant activity in honey after heat treatment. Czech Journal of Food Sciences, 31(6): 601-606.
https://doi.org/10.17221/509/2012-CJFS
Shapla, U.M., Solayman, M., Alam, N., Khalil, M.I. & Gan, S.H. (2018). 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: Effects on bees and human health. Chemistry Central Journal, 12(35): 1-18.
https://doi.org/10.1186/s13065-018-0408-3
Shen, Q., Zhang, B., Xu, R., Wang, Y., Ding, X. & Li, P. (2010). Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01. Anaerobe, 16(4): 380-386.
https://doi.org/10.1016/j.anaerobe.2010.06.006
Singh, I. & Singh, S. (2018). Honey moisture reduction and its quality. Journal of Food Science and Technology, 55(10): 3861-3871.
https://doi.org/10.1007/s13197-018-3341-5
Stojković, M., Cvetković, D., Savić, A., Topalić-Trivunović, L., Velemir, A., Papuga, S. & Žabić, M. (2020). Changes in the physicochemical, antioxidant and antibacterial properties of honeydew honey subjected to heat and ultrasound pretreatments. Journal of Food Science and Technology, 58: 2555-2566.
https://doi.org/10.1007/s13197-020-04762-2
Subramanian, R., Hebbar H.U. & Rastogi, N.K. (2007). Processing of honey: A review. International Journal of Food Properties, 10(1): 127-143.
https://doi.org/10.1080/10942910600981708
Sulaiman, N.H.I. & Sarbon, N.M. (2020). Physicochemical, antioxidant and antimicrobial properties of selected Malaysian honey as treated at different temperature: A comparative study. Journal of Apicultural Research, 1-9.
https://doi.org/10.1080/00218839.2020.1846295
Turkmen, N., Sari, F., Poyrazoglu, E.S. & Velioglu, Y.S. (2006). Effects of prolonged heating on antioxidant activity and colour of honey. Food Chemistry, 95(4): 653-657.
https://doi.org/10.1016/j.foodchem.2005.02.004
Turhan, I., Tetik, N., Karhan, M., Gurel, F. & Tavukcuoglu, H.R. (2008). Quality of honeys influenced by thermal treatment. LWT-Food Science and Technology, 41(8): 1396-1399.
https://doi.org/10.1016/j.lwt.2007.09.008
Wiegand, I., Hilpert, K. & Hancock, R.E.W. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols, 3(2): 163-175.
https://doi.org/10.1038/nprot.2007.521
Yan, S., Sun, M., Zhao, L., Wang, K., Fang, X., Wu, L. & Xue, X. (2019). Comparison of differences of α‑dicarbonyl compounds between naturally matured and artificially heated acacia honey: Their application to determine honey quality. Journal of Agricultural and Food Industry, 67: 12885-12894.
https://doi.org/10.1021/acs.jafc.9b05484
Zarei, M., Fazlara, A. & Tulabifard, N. (2019). Effect of thermal treatment on physicochemical and antioxidant properties of honey. Heliyon, 5(6): 1-6.
https://doi.org/10.1016/j.heliyon.2019.e01894
Zhishen, J., Mengcheng, T. & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4): 555-559.
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