Banana (Musa acuminata), Orange (Citrus reticulata), and Watermelon (Citrullus lanatus) Peels as Prebiotic

  • SUI SIEN LEONG Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Science, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • ERRA FAZIRA ABDUL RAHIM Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Science, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • SHAHRUL RAZID SARBINI Department of Crop Science, Faculty of Agricultural and Forestry Science, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • KAMIL LATIF Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Science, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • MASNINDAH MALAHUBBAN Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Science, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
Keywords: Fruit waste powder, Lactobacillus, mineral, prebiotics, proximate

Abstract

Fruit waste is being studied as a non-conventional alternative source of nutritional and mineral content that might be employed as functional food ingredients. This study aims to identify the 1) proximate and mineral composition of banana, orange and watermelon waste powder subjected to different drying methods; 2) prebiotic potential of the fruit waste powder (FWP) in growth enhancement of the probiotic Lactobacillus casei. The fruit peels were processed by two methods: freeze-dried and oven-dried. All FWP was sterilised and milled into particle size <180μm. The proximate (total ash, crude protein, crude fat, crude fibre), mineral (Ca, Zn, Na, K, Mg, Cu) profiling was analysed in triplicate according to standard. Prebiotic activities of FWP were determined through the growth of L. casei analysed. Significant differences (p<0.05) result was observed between the proximate and mineral parameters in all FWP. Watermelon FWP had the highest moisture, ash, sodium, potassium, phosphorus, and zinc content, while banana FWP contained the highest crude protein, crude fat, and magnesium content. Both banana and watermelon FWP were found to exhibit high crude fibre content. The orange WP was reported with the highest carbohydrate, calcium, and copper content. Although significant differences (p<0.05) in composition were noted, the oven and freeze-drying methods employed showed no pronounced effect. Calcium, copper content (all FWP), sodium and phosphorus (watermelon FWP), phosphorus (banana FWP) examined highly exceeded the recommended dietary allowance (RDA) limit. Banana FWP showed the highest L. casei net growth of log10 8.28±0.02– 8.36±0.01 CFU/mL and 91.61–98.66% of survival rate, thus showing its potential as prebiotic agents among other FWP. All types of FWP showed significant difference (p<0.05) in bacterial growth except for oven-dried orange FWP. Overall, the results revealed that all these fruit wastes could be exploited for the nutrient and value-added potential in food formulations due to their inexpensiveness, natural, safe, and environmental friendliness.

References

Adetoro, A.O., Opara, U.L. & Fawole, O.A. (2020). Effect of hot-air and freeze-drying on the quality attributes of dried pomegranate (Punica granatum L.) arils during long-term cold storage of whole fruit. Agriculture, 10: 493.

Akubor, P.I. & John, I.E.Z.E. (2012). Quality evaluation and cake making potential of sun and oven-dried carrot fruit. International Journal of Biosciences, 2: 19-27.

Al-Hindi, R.R. & Abd El Ghani, S. (2020). Production of functional fermented milk beverages supplemented with pomegranate peel extract and probiotic lactic acid bacteria. Journal of Food Quality, 2020: 1-9.

Association of Official Agricultural Chemists (AOAC). (2005). Official Method of Analysis of the Association of the Analytical Chemists. Maryland, USA: Association of Official Analytical Chemists Press.

Barbosa-Canovas, G., Ortega-Rivas, E., Juliano, P. & Yan, H. (2005). Food Powders: Physical Properties, Processing and Functionality. New York, NY, USA: Kluwer Academic/Plenum Publisher.

Bis-Souza, C.V., Penna, A.L.B. & da Silva Barretto, A.C. (2020). Applicability of potentially probiotic Lactobacillus casei in low-fat Italian type salami with added fructooligosaccharides: In vitro screening and technological evaluation. Meat Science, 168: 108186.

Chaouch, M.A. & Benvenuti, S. (2020). The role of fruit by-products as bioactive compounds for intestinal health. Foods, 9: 1716.

Chatterjee, E. & Manuel, S.G.A. (2016). Effect of fruit pectin on growth of lactic acid bacteria. Journal of Probiotics and Health, 4: 147.

Czech, A., Zarycka, E., Yanovych, D., Zasadna, Z., Grzegorczyk, I. & Kłys, S. (2020). Mineral content of the pulp and peel of various citrus fruit cultivars. Biological Trace Element Research, 193: 555-563.

Davis, C.D. & Milner, J.A. (2009). Gastrointestinal microflora, food components and colon cancer prevention. The Journal of Nutritional Biochemistry, 20: 743–752.

Dayarathna, S.G.A.R.M. & Karunarathna, B. (2021). Effect of different fruit peel powders as natural fertilizers on growth of okra (Belmoschus esculentus L.). Journal of Agricultural Sciences – Sri Lanka, 16: 67-79.

Dhama, K., Khurana, S.K., Karthik, K., Tiwari, R., Malik, Y.P.S. & Chauhan, R.S. (2014). Panchgavya: Immune-enhancing and therapeutic perspectives. Journal of Immunology and Immunopathology, 16: 1–11.

do Espírito Santo, A.P., Cartolano, N.S., Silva, T.F., Soares, F.A., Gioielli, L.A., Perego, P., Converti, A. & Oliveira, M.N. (2012). Fibers from fruit by- products enhance probiotic viability and fatty acid profile and increase CLA content in yoghurts. International Journal of Food Microbiology, 154: 135-144.

Garcia-Amezquita, L.E., Tejada-Ortigoza, V., Serna-Saldivar, S.O. & Welti-Chanes, J. (2018). Dietary fiber concentrates from fruit and vegetable by-products: Processing, modification, and application as functional ingredients. Food Bioprocess Technology, 11: 1439-1463.

Gladvin, G., Sudhaakr, G., Swathi, V. & Santhisri, K.V. (2017). Mineral and vitamin compositions contents in watermelon peel (rind). International Journal of Current Microbiology and Applied Sciences, 5: 129-133.

Hammes, W.P. & Hertel, C. (2006). The genera Lactobacillus and Carnobacterium. In Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. and Stackebrandt, E. (Eds.) The Prokaryotes: A Handbook on the Biology of Bacteria. New York: Springer. Pp. 320–403.

Hassan, H.F., Hassan, U.F., Usher, O.A., Ibrahim, A.B. & Tabe, N.N. (2018). Exploring the potentials of banana (Musa Sapietum) peels in feed formulation. International Journal of Advanced Research, 5: 10-14.

Hebert, E.M., Raya, R.R. & De Giori, G.S. (2000). Nutritional requirements and nitrogen-dependent regulation of proteinase activity of Lactobacillus helveticus CRL 1062. Applied and Environmental Microbiology, 66(12): 5316–5321.

Ho, L., Suhaimi, M.A., Ismail, I. & Mustafa, A.M. (2016). Effect of different drying conditions on proximate compositions of red and yellow fleshed watermelon rind powders. Journal of Agrobiotechnology, 7: 1-12.

Karami, S., Roayaei, M., Hamzavi, H., Bahmani, M., Hassanzad-Azar, H., Leila, M. & Rafieian-Kopaei, M. (2017). Isolation and identification of probiotic Lactobacillus from local dairy and evaluating their antagonistic effect on pathogens. International Journal of Pharmaceutical Investigation, 7: 137-141.

Kaur, A.P., Bhardwaj, S., Dhanjal, D.S., Nepovimova, E., Cruz-Martins, N., Kuča, K., Chopra, C., Singh, R., Kumar, H., Șen, H., Kumar, V., Verma, R. & Kumar, D. (2021). Plant prebiotics and their role in the amelioration of diseases. Biomolecules, 11: 440.

Kumbhar, J.V., Rajwade, J.M. & Paknikar, K.M. (2015). Fruit peels support higher yield and superior quality bacterial cellulose production. Applied Microbiology and Biotechnology, 99: 6677-6691.

Kumbhar, J.V., Rajwade, J.M. & Paknikar, K.M. (2015). Fruit peels support higher yield and superior quality bacterial cellulose production. Applied Microbiology and Biotechnology, 99(16): 6677–6691.

Lingoh, A.D., Leong, S.S. & Sarbini, S.R. (2020). Detection of lactic acid bacteria (LAB) from local breed chicken gut as probiotic agent in livestock. Pakistan Journal of Nutrition, 19: 197-203.

Liu, K. (2019). Effects of sample size, dry ashing temperature and duration on determination of ash content in algae and other biomass. Algal Research, 40: 101486.

Mæhre, H., Dalheim, L., Edvinsen, G., Elvevoll, E. & Jensen, I.J. (2018). Protein determination—Method matters. Foods, 7: 5-15.

Markowiak, P. & Śliżewska, K. (2017). Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients, 9: 1021-1027.

M’hiri, N., Ioannou, I., Ghoul, M. & Mihoubi Boudhrioua, N. (2016). Phytochemical characteristics of citrus peel and effect of conventional and nonconventional processing on phenolic compounds: A review. Food Reviews International, 33: 587-619.

Mirhosseini, H. & Amid, B.T. (2013). Effect of different drying techniques on flow ability characteristics and chemical properties of natural carbohydrate-protein gum from durian fruit seed. Chemistry Central Journal, 7: 1.

Mokhtar, S.M., Swailam, H.M. & Embaby, H.E. (2018). Physicochemical properties, nutritional value and techno-functional properties of goldenberry (Physalis peruviana) waste powder concise title: Composition of goldenberry juice waste. Food Chemistry, 248: 1-7.

Morais, D.R., Rotta, E.M., Sargi, S.C., Bonafe, E.G., Suzuki, R.M., Souza, N.E. & Visentainer, J.V. (2017). Proximate composition, mineral contents and fatty acid composition of the different parts and dried peels of tropical fruits cultivated in Brazil. Journal of the Brazilian Chemical Society, 28: 308-318.

Morris, A., Barnett, A. & Burrows, O. (2004). Effect of processing on nutrient content of foods. Cajanus, 37: 160-164.

Omotoso, O. T. & Adedire, C. O. (2007). Nutrient composition, mineral content and the solubility of the proteins of palm weevil, Rhynchophorus phoenicis. (Coleoptera: Curculionidae). Journal of Zhejiang University Science B, 8: 318-322.

Pathak, P.D., Mandavgane, S.A. & Kulkarni, B.D. (2017). Fruit peel waste: Characterization and its potential uses. Current Science, 113: 444-454.

Pyar, H. & Peh, K.K. (2018). Chemical compositions of banana peels (Musa sapientum) fruits cultivated in Malaysia using proximate analysis. Research Journal of Chemistry and Environment, 22: 108-113.

Radha, Kumar, M., Puri, S., Pundir, A., Bangar, S.P., Changan, S., Choudhary, P., Parameswari, E., Alhariri, A., Samota, M.K., Damale, R.D., Singh, S., Berwal, M.K., Dhumal, S., Bhoite, A.G., Senapathy, M., Sharma, A., Bhushan, B. & Mekhemar, M. (2021). Evaluation of nutritional, phytochemical, and mineral composition of selected medicinal plants for therapeutic uses from cold desert of western Himalaya. Plants, 10: 1429.

Rafiq, S., Singh, B. & Gat, Y. (2019). Effect of different drying techniques on chemical composition, and antioxidant properties of kinnow (Citrus reticulata) peel. Journal of Food Science and Technology, 56: 2458-2466.

Rawi, M.H., Zaman, S.A., Pa'ee, K.F., Leong, S.S. & Sarbini, S.R. (2020). Prebiotics metabolism by gut-isolated probiotics. Food Science Technology, 57: 2786-2799.

Recommended Dietary Allowances (RDA). (1989). National Research Council (US) Subcommittee on the Tenth Edition of the Recommended Dietary Allowances. Washington (DC): National Academies Press.

Rohana, S., Yatim, M., Halim, K., Hamid, K., Ismail, K.N., Rashid, Z.A. & Shafie, F.A. (2019). Study on waste generation and composition in rapid residential development of sub urban area in Kuala Selangor district, Selangor. Journal of Wastes and Biomass Management, 1: 1-5.

Romelle, F.D., Ashwini, Rani, P. & Manohar, R.S. (2016). Chemical composition of some selected fruit peels. European Journal of Food Science and Technology, 4: 12-21.

Salgaço, M.K., Perina, N.P., Tomé, T.M., Mosquera, E.M.B., Lazarini, T., Sartoratto, A. & Sivieri, K. (2021). Probiotic infant cereal improves children’s gut microbiota: Insights using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®). Food Research International, 143: 110292.

Sendra, E., Fayos, P., Lario, Y., Fernández-López, J., Sayas-Barberá, E. & Pérez-Alvarez, J.A. (2008). Incorporation of citrus fibers in fermented milk containing probiotic bacteria. Food Microbiology, 25: 13-21.

Sogi, D.S., Siddiq, M., Greiby, I. & Dolan, K.D. (2013). Total phenolics, antioxidant activity, and functional properties of ‘Tommy Atkins’ mango peel and kernel as affected by drying methods. Food Chemistry, 141(3): 2649–2655.

Suarni, M.A. & Firmansyah, I.U. (2008). Effect of drying temperature on nutritional quality of protein maize. 10th Asian Regional Maize Workshop Proceedings, 80–82.

Tripathy, A., Dash, J., Kancharla, S., Kolli, P., Mahajan, D., Senapati, S. & Jena, M.K. (2021). Probiotics: A promising candidate for management of colorectal cancer. Cancers, 13: 3178.

Ummadi, M.S. & Curic-Bawden, M. (2008). Use of protein hydrolysates in industrial starter culture fermentations. In Protein hydrolysates in biotechnology (pp. 91–114). Springer.

Wilkowska, A., Nowak, A., Antczak-Chrobot, A., Motyl, I., Czyżowska, A. & Paliwoda, A. (2019). Structurally different pectic oligosaccharides produced from apple pomace and their biological activity in vitro. Foods (Basel, Switzerland), 8: 365.

Zahid, H.F., Ranadheera, C.S., Fang, Z. & Ajlouni, S. (2021). Utilization of mango, apple and banana fruit peels as prebiotics and functional ingredients. Agriculture, 11: 584.

Published
2022-06-30
How to Cite
LEONG, S. S., ABDUL RAHIM, E. F., SARBINI, S. R., LATIF, K., & MALAHUBBAN, M. (2022). Banana (Musa acuminata), Orange (Citrus reticulata), and Watermelon (Citrullus lanatus) Peels as Prebiotic. Borneo Journal of Resource Science and Technology, 12(1), 81-94. https://doi.org/10.33736/bjrst.4528.2022