Morphological Characterisation and Documentation of Freshwater Macrophytes in Pontian, Johor's Water

  • YIN SING ONG Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • SING TUNG TENG Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Keywords: Freshwater macrophyte, intraspecific variation, Malaysia, morphology


Freshwater macrophytes exist in varied life forms for example, emergent, submerged, floating-leaved and free-floating, and some of them have a heterophylly or multiple forms. The intraspecific variation in terms of morphology is common phenomena in macrophytes population; however, specific studies on morphology of freshwater macrophytes are still insufficient, especially in Pontian, Johor. Hence, this study aimed to characterise the freshwater macrophytes based on morphometrics and descriptive characteristics as well as to document their diversity and population. Thus, the morphometric measurements were done, the descriptive characteristics were documented in scientific photographs, the population of freshwater macrophytes was also estimated by using quadrat estimation technique. Additionally, the morphometrics of some collected samples were compared with the same sample in other studies to show the variation in the range of different measurements, the descriptive characteristics were also described as well as the population estimation assessed by area coverage was analysed in the form of statistical chart. The finding in this study showed variation in morphometric data and no intraspecific variation on phenotype of freshwater macrophytes in Pontian, Johor’s water.


Ayeni, O., Jimoh, M. & Saheed, A. (2015). Inflorescence and floral characters indicating C3 and C4 photosynthesis in some species of the genus Cyperus L. (Cyperaceae). International Journal of Biological and Chemical Sciences, 9: 1844. Available from: DOI: 10.4314/ijbcs.v9i4.10

Bidarlord, M., Jalili, A. & Zamani, R. (2019). First record of Pistia stratiotes (Water Lettuce) from Gilan Province (North of Iran). Rostaniha, 20(2): 182-187. Available from: DOI: 10.22092/botany.2019.128011.1176

Bowden, W.B., Glime, J.M. & Riis, T. (2017). Chapter 13 - Macrophytes and bryophytes. In Hauer, F.R. & Lamberti, G.A (Eds.), Methods in stream ecology, Volume 1 (Third Edition) (pp. 243-271). Boston: Academic Press.

Capers, R. (2000). A comparison of two sampling techniques in the study of submersed macrophytes richness and abundance. Aquatic Botany, 68: 87-92. Available from: DOI: 10.1016/S0304-3770(00)00102-9

Ceschin, S., Abati, S., Leacche, I. & Zuccarello, V. (2018). Ecological comparison between duckweeds in Central Italy: The invasive Lemna minuta vs the native L. minor. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 152(4): 674-683. Available from: DOI: 10.1080/11263504.2017.1317671

Ceschin, S., Leacche, I., Pascucci, S. & Abati, S. (2016). Morphological study of Lemna minuta Kunth, an alien species often mistaken for the native L. minor L. (Araceae). Aquatic Botany, 131: 51-56. Available from: DOI: 10.1016/j.aquabot.2016.01.005

Chapman, D., Coetzee, J., Hill, M., Hussner, A., Netherland, M., Pescott, O., Stiers, I., van Valkenburg, J. & Tanner, R. (2017). Pistia stratiotes L. Bulletin OEPP/EPPO Bulletin, 47: 537-543. Available from: DOI: 10.1111/epp.12429

Chavan, D.P., Survase, S.A. & Kunjalwar, S.J. (2014). Taxonomic study of genus Eleocharis R. BR. from Vidarbha Region of Maharashtra. Journal of Global Biosciences, 3(4): 744-748.

Csiky, J., Szalontai, B., Mesterhazy, A. & Olah, E. (2010). A morphological study of Ceratophyllum tanaiticum, a species new to the flora of Hungary. Preslia -Praha-, 82: 247-259.

Das, S. & Goswami, S. (2015). Physiological responses of water hyacinth, Eichhornia crassipes (Mart.) solms, to cadmium and its phytoremediation potential. Turkish Journal of Biology, 40: 94. Available from: DOI: 10.3906/biy-1411-86

de Assis Murillo, R., Corrêa Alves, D., dos Santos Machado, R., Silveira, M.J., Fidanza Rodrigues, K. & Thomaz, S.M. (2019). Responses of two macrophytes of the genus Polygonum to water level fluctuations and interspecific competition. Aquatic Botany, 157: 10-16. Available from: DOI:

Dibble, E.D. & Thomaz, S.M. (2009). Use of fractal dimension to assess habitat complexity and its influence on dominant invertebrates inhabiting tropical and temperate macrophytes. Journal of Freshwater Ecology, 24(1): 93-102. Available from: DOI: 10.1080/02705060.2009.9664269

Ferreiro, N., Giorgi, A. & Feijoó, C. (2013). Effects of macrophyte architecture and leaf shape complexity on structural parameters of the epiphytic algal community in a Pampean Stream. Aquatic Ecology, 47: 389-401. Available from: DOI: 10.1007/s10452-013-9452-1

Galal, T.M. & Farahat, E.A. (2015). The invasive macrophyte Pistia stratiotes L. as a bioindicator for water pollution in Lake Mariut, Egypt. Environmental Monitoring and Assessment, 187(11): 701. Available from: DOI: 10.1007/s10661-015-4941-4

Gopal, B. & Goel, U. (1993). Competition and allelopathy in aquatic plant communities. The Botanical Review, 59(3): 155-210. Available from: DOI: 10.1007/BF02856599

Harped, D.M., Adams, C. & Mavuti, K. (1995). The aquatic plant communities of the Lake Naivasha wetland, Kenya: pattern, dynamics and conservation. Wetlands Ecology and Management, 3(2): 111-123. Available from: DOI: 10.1007/BF00177693

Ho Thanh, T. (2012). Water Hyacinth (Eichhornia crassipes) – Biomass production, ensilability and feeding value to growing cattle. PhD Thesis. Swedish University of Agricultural Sciences.

Howard, G., Hyde, M. & Bingham, M. (2016). Alien Limnobium laevigatum (Humb. & Bonpl. ex Willd.) Heine (Hydrocharitaceae) becoming prevalent in Zimbabwe and Zambia. BioInvasions Records, 5: 221-225. Available from: DOI: 10.3391/bir.2016.5.4.05

Huang, X., Wang, L., Guan, X., Gao, Y., Liu, C. & Yu, D. (2017). The root structures of 21 aquatic plants in a macrophyte-dominated lake in China. Journal of Plant Ecology, 11: 1-8. Available from: DOI: 10.1093/jpe/rtx018

Ismail, S., Abd Hamid, M. & Mansor, M. (2018). Ecological correlation between aquatic vegetation and freshwater fish populations in Perak River, Malaysia. Biodiversitas, 19: 279-284. Available from: DOI: 10.13057/biodiv/d190138

Jeppesen, E., Jensen, J.P., Søndergaard, M., Lauridsen, T., Pedersen, L.J. & Jensen, L. (1997). Top-down control in freshwater lakes: The role of nutrient state, submerged macrophytes and water depth. Hydrobiologia, 342: 151-164. Available from: DOI: 10.1023/A:1017046130329

Kornijów, R., Kuoppamäki, K., Horppila, J., Luokkanen, E. & Kairesalo, T. (2004). Impacts of a submerged plant (Elodea canadensis) on interactions between roach (Rutilus rutilus) and its invertebrate prey communities in a lake littoral zone. Freshwater Biology, 50: 262-276. Available from: DOI: 10.1111/j.1365-2427.2004.01318.x

Les, D. (1993). Ceratophyllaceae. In Flowering plants· Dicotyledons (pp. 246-250): Springer.

Li, W., Willby, N. & Hilt, S. (2017). Macrophytes in freshwater habitats-perspectives from Asia proceeding of the second international symposium of aquatic plant biology (Macrophytes 2014, Wuhan, China). Aquatic Botany, 140: 45-47. Available from: DOI: 10.1016/j.aquabot.2017.04.005

Liang, Y.S., Wang, J.C. & Chen, C.H. (2012). Taxonomic revision of Lindernia all. (Scrophulariaceae sensu lato) in Taiwan. Taiwan Journal of Forest Science, 27: 95-116.

Liu, L., Quan, H., Dong, B.C., Bu, X. Q., Li, L., Liu, F. D., Lei, G.C. & Li, H.L. (2016). Nutrient enrichment alters impacts of Hydrocotyle vulgaris invasion on native plant communities. Scientific Reports, 6(1): 39468. Available from: DOI: 10.1038/srep39468

Maine, M., Suñe, N., Panigatti, M., Pizarro, M. & Emiliani, F. (1999). Relationships between water chemistry and macrophyte chemistry in lotic and lentic environments. Archiv fur Hydrobiologie, 145: 129-145.

Moody, M., Les, D. & DiTomaso, J.M. (2008). The role of plant systematics in invasive aquatic plant management. Journal of Aquatic Plant Management, 46: 7-15.

National Committee on Invasive Alien Species Malaysia (2018). Invasive alien species in Malaysia. Perpustakaan Negara Malaysia, pp. 113.

Oyedeji, A. & Abowei, J. (2012). The classification, distribution, control and economic importance of aquatic plants. International Journal of Fisheries and Aquatic Science, 1(2): 118-128.

Paolacci, S., Harrison, S. & Jansen, M.A.K. (2016). A comparative study of the nutrient responses of the invasive duckweed Lemna minuta, and the native, co-generic species Lemna minor. Aquatic Botany, 134: 47-53. Available from: DOI:

Richards, J.H. & Lee, D.W. (1986). Light Effects on Leaf morphology in water hyacinth (Eichhornia crassipes). American Journal of Botany, 73(12), 1741-1747. Available from: DOI:


Roznere, I. & Titus, J. (2017). Zonation of emergent freshwater macrophytes: responses to small-scale variation in water depth. The Journal of the Torrey Botanical Society, 144: 254-266. Available from: DOI: 10.3159/TORREY-D-16-00017.1

Schneider, E. & Williamson, P. (1993). Nymphaeaceae. In flowering plants· Dicotyledons (pp. 486-493): Springer.

Sharip, Z., Schooler, S., Hipsey, M. & Hobbs, R. (2012). Eutrophication, agriculture and water level control shift aquatic plant communities from floating-leaved to submerged macrophytes in Lake Chini, Malaysia. Biological Invasions, 14: 1029-1044. Available from: DOI: 10.1007/s10530-011-0137-1

Silva, J. & Leite, A. (2011). Reproductive biology and pollination of Cabomba aquatica (Cabombaceae). Rodriguesia, 62: 919-926. Available from: DOI: 10.1590/S2175-78602011000400017

Siti-Munirah, M.Y. & Chew, M.Y. (2010). Cabombaceae, a new family record for peninsular Malaysia. Malayan Nature Journal, 62(3): 241-248. Tucker, G.C. (1983). The taxonomy of Cyperus (Cyperaceae) in Costa Rica and Panama. Systematic Botany Monographs, 2: 1-85. Available from: DOI: 10.2307/25027589

Wang, J., Wang, W., Xiong, J., Li, L., Zhao, B., Sohail, M. & He, Z. (2021). A constructed wetland System with aquatic macrophytes for cleaning contaminated runoff/storm water from urban area in Florida. Journal of Environmental Management, 280: 111794. Available from: DOI: 10.1016/j.jenvman.2020.111794

Wang, M.Z., Bu, X.Q., Li, L., Dong, B.C., Li, H.L. & Yu, F.H. (2018). Constraints on the evolution of phenotypic plasticity in the clonal plant Hydrocotyle vulgaris. Journal of Evolutionary biology, 31(7): 1006-1017. Available from: DOI:

Wannan, B. (2019). New records of Lindernia s.l. (Linderniaceae) for Eastern Australia. Australian Systematic Botany, 32: 123-133. Available from: DOI:10.1071/SB18022

Wetzel, R.G. (1992). Wetlands as metabolic gates. Journal of Great Lakes Research. 18(4). 529-532. Available from: DOI:

How to Cite
ONG, Y. S., & TENG, S. T. (2022). Morphological Characterisation and Documentation of Freshwater Macrophytes in Pontian, Johor’s Water. Borneo Journal of Resource Science and Technology, 12(1), 1-22.