Screening of Indole-3-Acetic Acid (IAA) Productions by Endophytic Fusarium oxysporum Isolated from Phyllanthus niruri

  • Abdul Raffi Junaidi
  • Mohamad Hasnul Bolhassan
Keywords: Phyllathus niruri, endophytic Fusarium oxysporum, IAA, Salkowski’s methods


Ten newly isolated endophytic fungi from Phyllathus niruri Linn. were identified as Fusarium oxysporum. These isolates were screened out for their productivity of indole acetic acid (IAA) by the salkowski's method. Out of these isolates, two isolates showed high amount of indole acetic acid production, which were FO9 and FO10 with concentration of 23.52 μg/ml and 5.95 μg/ml, respectively.


Fierro-Coronado, R.A., Quiroz-Figuero, F.R., Garcia-Pérez, L.M., Ramirez-Chávez, E., Molina-Torres, J. & Maldonado-Mendoza, I.E. (2014). IAA-producing rhizobacteria from chickpea (Cicer arietinum L.) induce changes in root architecture and increase root biomass. Canadian Journal of Microbiology, 60(10): 639-648.

Aimé, S., Alabouvette, C., Steinberg, C. & Olivain, C. (2013). The endophytic strain Fusarium oxysporum Fo47: A good candidate for priming the defense responses in tomato roots. Molecular Plant-Microbe Interaction, 26(8): 918-926.

Bacon, C.W. & Yates, I.E. (2006). Endophytic root colonization by Fusarium Species: Histology, plant interactions, and toxicity. In Microbial Root Endophytes, 9: 133-152.

Bhagobaty, R.K. & Joshi, S.R. (2009). Promotion of seed germination of green gram and chick pea by Penicillium verruculosum RS7PF, a root endophytic fungus of Potentilla fulgens L. Advance Biotech, 8(12): 7-15.

Bhore, S.J., Ravichantar, N. & Loh, C.Y. (2010). Screening of endophytic bacteria isolated from leaves of Sambung Nyawa [Gynura procumbens (Lour.) Merr.] for cytokinin-like compounds. Bioinformation, 5(5): 191-197.

Cohen, B.A, Amsellem, Z., Maor, R., Sharon, A. & Gressel, J. (2002). Transgenically enhanced expression of indole-3-acetic Acid confers hypervirulence to plant pathogens. Phytopathology, 92(6): 590-596.

Dasri, K., Kaewharn, J., Kanso, S. & Sangchanjiradet, S. (2014). Optimization of indole-3-acetic acid (IAA) production by rhizobacteria isolated from epiphytic orchids. KKU Research Journal, 19(6): 268-275.

Glickmann, E. & Dessaux, Y. (1995). A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 61(2), 793-796.

Goveas, S.W., Madtha, R., Nivas, S.K. & D'Souza, L. (2011). Isolation of endophytic fungi from Coscinium fenestratum (Gaertn.) Colber. a red listed endangered medicinal plant. EurAsian Journal of Biosciences, 48-53.

Hasan, H.A.H. (2002). Gibberellin and auxinindole production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Rostlinna Vyroba, 48(3): 101-106.

Kedar, A., Rathod, D., Yadav, A., Agarkar, G. & Rai, M. (2014). Endophytic Phoma sp. isolated from medicinal plants promote the growth of Zea mays. Nusantara Bioscience, 6(2): 132-139.

Khalid, A., Arshad, M. & Ahmad Zahir, Z. (2003). Growth and yield response of wheat to inoculation with auxin producing plant growth promoting rhizobacteria. Pakistan Journal of Botany, 35(4): 483-498.

Khan, A. L., Hamayun, M., Kang, S.-M., Kim, Y.-H., Jung, H.-Y., Lee, J.-H. & Lee, I.-J. (2012). Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiology, 12(3), 1-14.

Martinuz, A., Schouten, A. & Sikora, R.A. (2013). Post-infection development of Meloidogyne incognita on tomato treated with the endophytes Fusarium oxysporum strain Fo162 and Rhizobium etli strain G12. BioControl, 58: 95-104.

Mohite, B. (2013). Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of Soil Science and Plant Nutrition, 13(3): 638-649.

Naik, A.D. & Juvekar, A.R. (2003). Effects of alkaloidal extract of Phyllanthus niruri on HIV replication. Indian Journal of Medical Sciences, 57(9): 387-393.

Paithankar, V.V., Raut, K.S., Charde, R.M. & Vyas, J.V. (2011). Phyllanthus Niruri!: A magic Herb. Research in Pharmacy, 1(4): 1-9.

Prasad, M.P. & Dagar, S. (2014). Identification and characterization of Endophytic bacteria from fruits like Avacado and Black grapes. International Journal of Current Microbiology and Applied Sciences, 3(8): 937-947.

Schulz, B. & Boyle, C. (2006). What are Endophytes? In Schulz, B.J.E., Boyle, C.J.C. & Sieber, Th.N. (Eds.), Microbial Root Endophytes. Berlin Heidelberg: Springer. Pp 1-13.

Meudt, W.J. & Gainess, T.P. (1967). Studies on the oxidation of Indole-3- acetic acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic acid oxidation products. Plant Physiology, 42: 1395-1399.

zum Felde, A., Mendoza, A., Cabrera, J.A., Kurtz, A., Schouten, A., Pocasangre, L., & Sikora, R. A. (2009). The burrowing nematode of banana: Strategies for controlling the uncontrollable. In Jones, D. & Van den Bergh, I. (Eds.), Proceeding of International Symposium on Banana Crop Prot. Sust. Prod. & Impr. Livelihood. Acta Horticulturae, 828, ISHS. Pp 101-108.

How to Cite
Junaidi, A. R., & Bolhassan, M. H. (1). Screening of Indole-3-Acetic Acid (IAA) Productions by Endophytic Fusarium oxysporum Isolated from Phyllanthus niruri. Borneo Journal of Resource Science and Technology, 7(1), 56-59.