Histological Alterations in Some Organs of African Catfish (Clarias gariepinus) Exposed to Sub-lethal Concentrations of Glyphosate [N-(phosphonomethyl) glycine]

  • Adedamola Akinbadewa Obafemi Awolowo University, Ile-Ife
  • Henry Adewole Obafemi Awolowo University, Ile-Ife
  • Stephen Adeyeni Obafemi Awolowo University, Ile-Ife
  • Victor Olaleye Obafemi Awolowo University, Ile-Ife
Keywords: Alterations, Clarias gariepinus, glyphosate, histology, organs


This study used a static bioassay to investigate the histological effects of glyphosate on the gill, liver and muscle of African catfish (Clarias gariepinus) fingerlings. This was done with a view of further characterising the effect of glyphosate on C. gariepinus fingerlings and other aquatic life forms. Six-week old C. gariepinus fingerlings with an average weight of 10.02 ± 0.2 g were stocked into three exposure sets (control, 2.75 ppm (25% of the 96 h LC50 value) and 5.50 ppm (50% of the 96 h LC50 value)) in triplicate at 30 fish per tank for 70 days. The 96 h LC50  value was 11.00 mg/L. Histological examination of the C. gariepinus exposed to various sublethal concentrations of glyphosate showed that major histological changes in their organs were concentration dependent such as gill arch vacuolation, excessive mucosal secretions, lifting of epithelial, and epithelium thickening, hyperplasia and telangiectasis in the gills, discolouration, change in form and consistent alterations involving hyperplasia, narrowing of the central nerve, necrosis, pkynosis, blood congestion and vacuolation of the liver, mild hyperplasia and inflammatory responses in the muscle of the fish. The severity of histological alteration was more pronounced in fish organs exposed to 5.50 ppm of glyphosate concentration. This study concluded that the toxicant (glyphosate) is highly toxic to C. gariepinus particularly at a concentration of 5.50 ppm, therefore its use near farm lands or adjacent water bodies should be discouraged.


Adedeji O. B., Adedeji A. O., Adeyemo O. K. & Agbede S. A. (2008). Acute toxicity of diazinon to the African catfish (Clarias gariepinus). African Journal of Biotechnology 7(5): 651–654.
Aliakbar H., Reza T., Ahmad S., Toktam B., Mohammad H. & Mohsen T. (2013). Median
lethal concentration of two pesticides, diazinon and deltamethrin, on tra catfish
Pangasius hypophthalmus (Siluroidei, Pangasiidae). Journal of Toxicology and Industrial Health. Pp.15-19
Ayanda, O.I. & Egbamuwo, E.A. (2012). Histopathological Examination of the liver and Gills
of Clarias gariepinus Treated with Glyphosate. Journal of Enviromental Research. 6 (3):228-234.
Ayoola, S.O. (2002). Toxicity of Glyphosate herbicide on Nile Tilapia (Oreochromis niloticus) Juvenile. Journal of Eco-toxicology and Environmental Safety. 7(4):
Ayoola S.O. (2008a). Toxicity of glyphosate herbicide on Nile tilapia (Oreochromis niloticus) juvenile. African Journal of Agricultural Research. 3(12):825-834, Available online at http://www.academicjournals.org/AJAR.
Ayoola, S.O. (2008b). Histopathological effects of Glyphosate on Juvenile African Catfish (Clarias gariepinus). European Journal of Agriculture and Environmental Science.
Bawa, V., Kondal, J.K., Hundal, S. S., & Harpinder K. (2017). Biochemical and Histological
Effects of Glyphosate on the Liver of Cyprinus carpio (Linn.). American Journal of Life Sciences. Environmental Toxicology 5(3-1):71-80.
Braunbeck, T. & Volkl, A. (1993). Toxicant-induced cytological alterations in the fish liver as biomarkers of environmental pollution? A case study on hepatocellular effects of dinitro-o-cresol in Golden Ide Leuciscus idus melanotus. In: Journal of Fish Eco-toxicology and Eco-physiology. Preceedings of an International Symposium, Heidelberg, September 1991. (Eds.) T. Braunbeck, W. Hanke, and H. Segner. VCH Verlagsgesellschaft mbH.Weinheim.
Carter, K., Rao, C., Lopez, A. D., & Taylor, R. (2012). Morality and cause of death
reporting and analysis systems in seven pacific Island countries. BMC Journal of Public Health 12: 436.
Camargo, M. M. P. & Martinez, C. B. R. (2007). Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology 5(3):327-336.
Cavas, T. & Konen, S. (2007). Detection of cytogenetic and DNA damage in peripheral
erythrocytes of goldfish (Carassius auratus) exposed to glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis 22:263-68.
Dutta, M. H., Munshi, D. J. & Roy K. P. (1994). Malathion induced changes in the lymphatic system of a catfish, Heteropneusles fossilis. Journal of Integrated
Comparative Biology 34 (5):50-53.
Figueiredo-Fernandes, A., Ferreira-Cardoso J. V., Garcia-Santos S., Monteiro S. M., CarrolaFolmar, L. C., Sanders, J. O. & Julin, A. M. (1979). Toxicity of the Herbicides Glyphosate and several of its formations to fish and Aquatic Invertebrates. Journal of Environmental Contamination Toxicology. 8: 269-278.
Folmar, L. C., Sanders, H. O. & Julin A. M. (1979). Toxicity of the herbicide glyphosphate and
several of its formulations to fish and aquatic invertebrates. Arch Environ ContamToxicol 8: 269-278.
Gernhöfer, M., Pawert, M., Schramm, M., Müller, E. & Triebskorn, R. (2001). Ultrastructural
biomarkers as tools to characterize the health status of fish in contaminated streams. J
Aquat Ecosyst Stress Recovery 8:241‑60.
Giesy, J.P., Dobson S., & Solomon K.R. (2000). Ecotoxicological risk assessment for Roundup
herbicide. Rev Environ Contam Toxicol 167:35‑120.
Hadi, A. A. & Alwan S. F. (2012). Histopathological changes in gills, liver and kidney of fresh water fish, Tilapia zillii, exposed to aluminium. International Journal of
Pharmacy and Life Science. 3(11): 2071-2081.
Heath, A. G. (1987).Water Pollution and Fish Physiology. CRC Press, Florida. Pp. 71 – 73.
Hued, A.C., Oberhofer S, & de los Ángeles-Bistoni M. (2012). Exposure to a commercial
glyphosate formulation (Roundup®) alters normal gill and liver histology and affects male sexual activity of Jenynsia multidentata (Anablepidae, yprinodontiformes). Arch Environ Contam Toxicol 62:107‑17.
Jiraungkoorskul W., Upatham E.S., Kruatrachue M., Sahaphong S., Vichasri-Grams S. &
Pokethitiyook P. (2002). Histopathological effects of Roundup, a glyphosate herbicide, on Nile tilapia (Oreochromis niloticus). Science Asia 28:121-127.
Ladipo, M.K., Doherty, V.F., & Oyebadejo, S.A. (2011). Acute Toxicity, Behavioural Changes and Histopathological Effect of paraquat Dichloride on Tissues of Catfish (Clarias gariepinus). International Journal of Biology. 3(2):67-74.
Luna, L.G (1968). Manual of Histologic Staining Method of the Armed Forces Institude of Pathology. New york: Migraw-Hill Pp. 73-76.
Mallatt, J. (1985). Fish gill structural changes induced by toxicants and other irritants: a statistical review. Canadian Journal of Fish and Aquatic Sciences, 42: 630-648.
Mohamed, F.A. (2009) Histopathological studies on Tilapia zillii and Solea vulguris from lake
Qarun, Egypt. World J Fish and Mari Sci 1: 29-39.
Mollendrof, F. (1973). Cytology and Cell Physiology. 3rd Edition. Academic Press. New
York. Pp. 48-51.
Myers, M. S., Johnson, L. L., Hom T., Coiler, T. K., Stein, J. E. & Varanasi, V. (1998).
Toxicopathic hepatic lesions in subadult English sole from Puget sound, WA; relationship to other indicators of contaminant exposure. Journal of Marine Environmental Resources 45:47-67.
Neibor, E., & Richardson, D. H. (1980). Replacement of non-descript term heavy metal by a biological and chemically significant classification of metal ions. Environmental Pollution Series 3(1): 24-45.
Neskovic, N.K., Poleksic, V., Elezovic, I., Karan,V. & Budimir, M. (1996). Biochemical and histopathological effects of Glyphosate on Carp (Cyprinus Carpio). Bulletin of Environmental Contamination Toxicology 56: 295- 302.
Nowak, B. (1992). Histological changes in gills induced by residues of endosulfan. Journal of
Aquatic Toxicology 23: 63-84.
Ogundiran M. A., Fawole O. O., Adewoye S. O. & Ayandiran T. A. (2009). Pathologic lesions
in the gills of Clarias gariepinus exposed to sublethal concentrations of soap and detergent effluents. Journal of Cell and Animal Biology 3 (5): 078-082. Available online at http://www.academicjournals.org/JCAB.
Okayi R.G., Aunue, P. A., Tachia, M. U. & Oshoke, O.J. (2010). Acute toxicity of
Glyphosate on Clarias gariepinus Fingerlings. Journal of Research in Forestry,
Wildlife and Environment. 2 (2): 150-154.
Olurin, K.B., Olojo, E.A.A., Mbaka, G., & Akindele, A. T. (2006). Histopathological responses of the gill and liver tissues of Clarias gariepinus fingerlings to the herbicide, Glyphosate. African Journal of Biotechnology 5 (24): 2480-2487.
Ortiz, R.M., Noren, D.P., Ortiz, C.L. & Talamantes, F. (2003). GTL and gherlin increase
with fasting in a naturally adapted species, the northern elephant seal (Mirounga angustirostris). Journal of Endocrinology 178: 533 – 539.
Pacheco, M. & Santos, M. A. (2002). Biotransformation, genotoxic and histopathological effects of environmental contaminants in European eel, Anguilla anguilla L. Eco-toxicology and Environmental Safety 53:331-347.
Ramírez-Duarte, W.F., Rondón-Barragán I.S. & Eslava-Mocha, P.R. (2008). Acute toxicity and
histopathological alterations of Roundup® herbicide on “cachama blanca” (Piaractus brachypomus). Pesq. Vet. Bras. 28(11):547-554
Reuters (2011). Roundup: Cancer Cause or Crucial for Food Production? The Huffington Post.
Reuters; Available from: http://www.huffingtonpost.com/2011/04/11/round‑up‑cancer‑cause_n_847423.html. [Last accessed on 2017 Mar 15].
Richmonds, C. & H. M. Dulta (1989). Histopatholigical changes induced by malathion in
the gills of blue gill. Lepomis macrochinis. Bulletin Environmental Contamination
Toxicology 43: 123-130.
Risbourg S. B. & Bastide J. (1995). Hepatic perturbations induced by a herbicide (atrazine) in
juvenile grey mullet, Liza ramada (Mugilidae Telostei): an ultra structural study. Journal of Aquatic Toxicology 31:217-229.
Ruppel, M.L., Brightwell, B.B., Schaefer, J. & Marvel J.T. (1977). Metabolism and
degradation of glyphosate in soil and water. J Agric Food Chem 25:517‑28.
Sabae S.Z., El-Sheekh M. M., Khalil, M. A., Elshouny, W. A. E. & Badr, H. M. (2014)
Seasonal and regional variation of physicochemical and bacteriological parameters of surface water in El-Bahr ElPherony, Menoufia, Egypt. World J Fish Marine Sci 6: 328-335.
Samanta P., Kumari P., Pal S., Mukherjee A.K., & Ghosh, A.R. (2018). Histopathological and
ultrastructural alterations in some organs of Oreochromis niloticus exposed to glyphosate-based herbicide, excel mera 71. J Microsc Ultrastruct 6:35-43.
Selvanathan, J., Vincent, S. & Nirmala, A. (2013). Histopathology changes in fresh water fish Clarias batirachius(linn.) exposed to Mercury and Cadmium. International Journal of Life Science and Pharmaceutical Research 3 (2): 34-38.
Sihtmae, M., Blinova, I., Kunnis-Beres, M., Kanarbik, L., Heinlaan, M. & Kahru, A. (2013)
Ecotoxicological effects of different glyphosate formulations. App Soil Ecol 72: 215–224.
Sullivan, K. M. & Somero, G. N. (1983). Size and diet related variations in enzyme activity and tissue compositions in the sablefish, Anoplopana Fimbria. Biological Bulletin of Marine Biology Laboratory 164:315-326.
Tomlin C.D.S (2006). The Pesticide Manual: A World Compendium. 14thedn. British Crop
Protection Council:Hampshire, UK. pp: 545-548.
Thanomsit, C., Wattanakornsiri A., & Nanthanawat, P. (2016). Effect of Glyphosate on Fish
Behavior and Histological Alteration of Gills in Asian Sea Bass (Lates calcarifer)
WHO (1994). Glyphosate. Environmental Health Criteria no.159, World Health Organization,
How to Cite
Akinbadewa, A., Adewole, H., Adeyeni, S., & Olaleye, V. (2021). Histological Alterations in Some Organs of African Catfish (Clarias gariepinus) Exposed to Sub-lethal Concentrations of Glyphosate [N-(phosphonomethyl) glycine]. Borneo Journal of Resource Science and Technology, 11(2), 62-75. https://doi.org/10.33736/bjrst.3815.2021