Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut

Authors

  • CARLINA FREDDIE SIMOL Faculty of Agricultural Sciences and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • JOHN KEEN CHUBO Faculty of Agricultural Sciences and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • PATRICIA JIE HUNG KING Faculty of Agricultural Sciences and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • KIAN HUAT ONG Faculty of Agricultural Sciences and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • CINDY CHEW Faculty of Agricultural Sciences and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia
  • KHALID NAWI Faculty of Agricultural Sciences and Forestry, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008 Bintulu, Sarawak, Malaysia

DOI:

https://doi.org/10.33736/bjrst.2879.2021

Keywords:

Ligninolytic enzyme, ligninolytic microbe, termite gut

Abstract

Ligninolytic microbes have great potential in converting high lignin by-products to more utilisable products by decomposing the lignin-rich agricultural and industrial wastes. Thus, the aim of this study are to screen and identify the potential ligninolytic microbes from the termite (Coptotermes curvignathus) gut. The study was conducted at Universiti Putra Malaysia Bintulu Sarawak Campus, Malaysia. Twenty-seven microbes isolated from termite gut obtained from the Microbiology Laboratory, Faculty of Agricultural Science and Forestry, were used for the ligninolytic activity screening. Media with four different ligninolytic indicator dyes (Azure B, phenol red, methylene blue, and Remazol Brilliant Blue) were streaked with microbial isolates and incubated at 37 °C for 48 h. Out of twenty-seven microbe isolates, only three (CH2, CH5, and CH9) isolates showed decolourisation zone indicating the positive presence of ligninolytic activity. The 16S rRNA gene sequence data indicated the isolates are highly homologous to Bacillus spp.

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Published

2021-06-30

How to Cite

FREDDIE SIMOL, C., CHUBO, J. K., KING, P. J. H., ONG, K. H., CHEW, C. ., & NAWI, K. (2021). Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut. Borneo Journal of Resource Science and Technology, 11(1), 35–42. https://doi.org/10.33736/bjrst.2879.2021