Chemosystematic Evaluation of Some Nigerian Gossypium hirsutum L. Using Qualitative and Quantitative Phytochemical Analysis
DOI:
https://doi.org/10.33736/bjrst.2728.2021Keywords:
Diversity, Gossypium hirsutum, phytochemicalsAbstract
There is little information on the use of phytochemicals for the systematic study of variation and resolving relatedness in Nigerian Gossypium hirsutum species. Thus, there is a need to carry out detailed phytochemical studies of the plant leaves, which could be helpful in ascertaining their diversity and relatedness. The qualitative and quantitative phytochemical screening was conducted on 18 accessions of G. hirsutum using the established laboratory protocols. The findings of this study indicated the presence of tannin, alkaloids, proline, flavonoids, and saponin in all of the accessions that were grouped into two at 17% genetic distance, indicating 83% relatedness. However, phytosterol, terpenoids and glycosides were absent in three of the accessions, which were grouped at 48%. The phytochemical compositions were tannin (1.30-1.09 mg/100 g), flavonoid (1.60-1.16 mg/100 g, terpenoid (0.42-0.35 mg/100 g), alkaloid (1.47-1.11 mg/100 g), saponin (4.86-1.59 mg/100 g), glycoside (1.02-0.88 mg/100 g), proline (1.57-1.0 mg/100 g) and phytosterol (0.36-0.2 mg/100 g). The aim of this research was to determine the diversity and relatedness of G. hirsutum genotypes collected from different locations of Nigeria. In general, this study emphasises the use of plant chemicals for classification purposes.
References
Amadi, B.A., Agomuo, E.N. & Ibegbulem, C.O. (2004). Research Methods in Biochemistry. Owerri: Supreme Publishers.
Akpabio, K.E. (1988). Crude protein electrophoresis of seeds of eight species of Crotalaria L. Nigeria Journal of Botany, 1: 106-110.
Atal, C. (1982). Cultivation and utilization of aromatic plants. 1st ed, New Delhi: Council of Scientific and Industrial Research. Pp 15-21.
Ayeni, M.J., Oyeyemi, S.D., Kayode, J. & Peter, G. P. (2015). Phytochemical, proximate and mineral analyses of the Leaves of Gossypium hirsutum L. and Momordica charantia L. Journal of Natural Sciences Research, 5(6): 99-107.
Ben, A.A., Anakaa, T.M. & Peters, G.C. (2017). Identification and taxonomic classification of plants in the family Portulacaceae in Dutsin-Ma local government area of Katsina State, Nigeria. International Journal of Innovative Research and Advanced Studies, 4(10): 146-149.
Boham, B.A. and Kocipal-Abyazan, R. (1994). Flanonoids and condensed tannins from leaves of Hawaiian Vaccinium reticulatum and V. calycinum. Pacific Science 48, Pp 458-463.
Chandrashekar, R., Kumar, A.K., Reddy, Y. R., Chaitanya, P.J., Bhavani, N. L. & Pochampalli, J. (2013). Isolation of gossypol and analysis of phytochemicals in seed extract of Bt and non-Bt varieties of cotton. Journal of Pharmacognosy and Phytochemistry, 2(1): 180-186.
Davis, P.A. & Heywood, VH. (1963). Principle of Angiosperm Taxonomy. Edinburgh: Oliver and Boyd. Pp 210-230.
Domingues, R. M., Kaita, M. C., Avelar, E., Sonza, K.E.S., Moraes, W.D.G.S & Franco, E.N. (1998). Electrophoretic characterization of exposed outer membrane proteins in environmental and human bacteroides fragilis strains. Zentralblatt für Bakteriologie, 287(4): 331-341.
https://doi.org/10.1016/S0934-8840(98)80167-9
Ejikeme, C. M., Ezeonu, C. S. & Eboatu, A. N. (2014). Determination of physical and phytochemical constituents of some tropical timbers indigenous to Niger Delta Area of Nigeria. European Scientific Journal, 10(8): 247-270.
Feliner, G.N. & Fernandez, I.A. (2000). Biosystematics in the 90s: Integrating data from different sources. Portugaliae Acta Biologica, 19: 9-19.
Harbourne, J. B. (1973). Phytochemical Methods. London: Chapman and Hall Ltd. Pp 49-188.
Harborne, J.B. (1993). Introduction to ecological biochemistry. London: Fourth Academic Press.
Hegnauer, R. (1986). Phytochemistry and plant taxonomy - An essay on the chemotaxonomy of higher plants. Phytochemistry, 25: 1519-1535.
https://doi.org/10.1016/S0031-9422(00)81204-2
Jones, B. and Luchsinger, A. (1987). Plant systematics. New York: Mc-Graw Hill Book Co.
Obadoni, B.O. & Ochuko, P.O. (2001). Phytochemical studies and efficacy of the extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Science, 8: 203-208.
https://doi.org/10.4314/gjpas.v8i2.16033
Odebiyi, A. & Sofowora, A.E. (1990). Phytochemical screening of Nigerian Medicinal Plants (Part III). Lloyida, 14: 234-246.
Pandey, B.P. (2007). A Text Book of Botany: Angiosperms: Taxonomy, Anatomy, Embryology (Including Tissue Culture) and Economic Botany. Second Edition. New Delhi: S. Chand and Company Limited, Pp 990.
Singh, R. (2016). Chemotaxonomy: A tool for plant classification. Journal of Medicinal Plant Studies, 4(2): 90-93.
Smith, M. A., Rottkamp, C. A., Nunomura, A., Raina, A. K. & Perry, G. (2000). Oxidative stress in Alzheimer's disease. Biochimica et Biophysica Acta-Molecular Basis of Disease, 1502(1): 139-144.
https://doi.org/10.1016/S0925-4439(00)00040-5
Smith, P. (1976). The chemotaxonomy of plants London. London: Edward Arnold (Publishers) Ltd.
Sofowora, E. A. (1993). Medicinal plants and traditional medicine in Africa. Ibadan, Nigeria: Spectrum Books Limited. Pp.150 -289.
Sharma, R & Kaur, S. (2017). Antimicrobial and phytochemical screening of Trikuta-traditional food of western Rajasthan. Indian Journal of Traditional Knowledge, 16(2): 270-276.
Sofowora, E. A. (1993). Medicinal plants and traditional medicine in Africa. Nigeria: Spectrum Books Limited, Pp.150 -289.
Stace, C. (1980). Plant Taxonomy and Biosystematics. London: Edward Arnold (Publishers) Ltd.
Trease, G.E. & Evans, W.C. (1989). Pharmacognosy. Nigeria: Nigerian Tirldercan Macmillian Publishers.
Umoh, O. (2020). Chemotaxonomy: The role of phytochemicals in chemotaxonomic delineation of taxa. Asian Plant Research Journal, 5(1): 43-52.
https://doi.org/10.9734/aprj/2020/v5i130100
Van-Burden, T. & Robinson, W. (1969). Formation of complexes between protein and tannin acid. Journal of Agricultural Food Chemistry, 17(4):772-777.
https://doi.org/10.1021/jf60164a003
Wink, M. and Waterman, P.G. (1999). Chemotaxonomy in relation to molecular phylogeny of plants. In Wink, M. and Waterman, P.G. (eds) Biochemistry of plant secondary metabolism. Sheffield Academic Press and CRC Press. Pp. 300-341.
Zheng, W. and Wang, S.Y. (2003). Oxygen Radical Absorbing Capacity of Flavonoids Phenolic Acids in Blueberry, Cranberry, Chokeberry and Liganberry. Journal of Agriculture and Food Chemistry, 51: 502-509.
Downloads
Published
How to Cite
Issue
Section
License
Copyright Transfer Statement for Journal
1) In signing this statement, the author(s) grant UNIMAS Publisher an exclusive license to publish their original research papers. The author(s) also grant UNIMAS Publisher permission to reproduce, recreate, translate, extract or summarize, and to distribute and display in any forms, formats, and media. The author(s) can reuse their papers in their future printed work without first requiring permission from UNIMAS Publisher, provided that the author(s) acknowledge and reference publication in the Journal.
2) For open access articles, the author(s) agree that their articles published under UNIMAS Publisher are distributed under the terms of the CC-BY-NC-SA (Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License) which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original work of the author(s) is properly cited.
3) For subscription articles, the author(s) agree that UNIMAS Publisher holds copyright, or an exclusive license to publish. Readers or users may view, download, print, and copy the content, for academic purposes, subject to the following conditions of use: (a) any reuse of materials is subject to permission from UNIMAS Publisher; (b) archived materials may only be used for academic research; (c) archived materials may not be used for commercial purposes, which include but not limited to monetary compensation by means of sale, resale, license, transfer of copyright, loan, etc.; and (d) archived materials may not be re-published in any part, either in print or online.
4) The author(s) is/are responsible to ensure his or her or their submitted work is original and does not infringe any existing copyright, trademark, patent, statutory right, or propriety right of others. Corresponding author(s) has (have) obtained permission from all co-authors prior to submission to the journal. Upon submission of the manuscript, the author(s) agree that no similar work has been or will be submitted or published elsewhere in any language. If submitted manuscript includes materials from others, the authors have obtained the permission from the copyright owners.
5) In signing this statement, the author(s) declare(s) that the researches in which they have conducted are in compliance with the current laws of the respective country and UNIMAS Journal Publication Ethics Policy. Any experimentation or research involving human or the use of animal samples must obtain approval from Human or Animal Ethics Committee in their respective institutions. The author(s) agree and understand that UNIMAS Publisher is not responsible for any compensational claims or failure caused by the author(s) in fulfilling the above-mentioned requirements. The author(s) must accept the responsibility for releasing their materials upon request by Chief Editor or UNIMAS Publisher.
6) The author(s) should have participated sufficiently in the work and ensured the appropriateness of the content of the article. The author(s) should also agree that he or she has no commercial attachments (e.g. patent or license arrangement, equity interest, consultancies, etc.) that might pose any conflict of interest with the submitted manuscript. The author(s) also agree to make any relevant materials and data available upon request by the editor or UNIMAS Publisher.