Effect of Media and Temperature on Growth and Preliminary Detection of Ligninolytic and Cellulolysic Activity of Trametes spp.
DOI:
https://doi.org/10.33736/bjrst.271.2012Keywords:
Trametes species, Trametes ljubarski, Trametes velutina, cellulolytic fungi, fungal growthAbstract
Effects of media and temperatures on growths of Trametes cervina (Schwein.) Bres., T. ljubarski Pilat, T. orientalis (Yasuda) Imazeki and T. velutina (Pers.) G. Cunn were studied. There was significant different of effect of media on growth of all the Trametes isolates. All of the four Trametes isolates were able to grow on Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Yeast Malt Agar (YMA) and Corn Meal Agar (CMA), however, no growth of T. cervina was seen on Czapek Dox Agar (CDA). Colony growth rates varied depending on the media and isolate of the fungi. T. orientalis showed the fastest growth while T. cervina showed the slowest growth on all the tested media. Although, T. ljubarski, T. orientalis and T. velutina can grow on CDA, the formation of mycelia was sparsely. There were significant differences of effect of temperature on growth of the Trametes isolates. T. orientalis and T. velutina, both, can grow at temperature up to 40oC, T. ljubarski can growth at temperature up to 35oC while T. cervina at temperature up to 30oC only. The optimum growth temperature for, both, T. orientalis and T. velutina was at 30oC, while for isolate T. ljubarski at 35oC and T. cervina at 25oC. All of the four Trametes isolates showed excellent cellulosic activity, indicated by the formation of clearing zone in the test media. The diameters of the degraded area formed by all of the Trametes isolates were more than 8 cm. T. orientalis and T. ljubarski degraded lignin better than T. cervina or T. velutinaReferences
Akhtar, M., Blanchette, R. A. & Kirk, T. K. (1997). Fungal delignification and Biomechanical Pulping of wood. Advances in Biochemical Engineering/ Biotechnology, 57: 159-195.
https://doi.org/10.1007/BFb0102074
Aust, A. D. & Benson, J. T. (1993). The Fungus among Us: Use of White Rot Fungi to Biodegrade Environmental. Pollutants. Environmental Health Perspectives, 101(3).
https://doi.org/10.1289/ehp.93101232
Burdsall, Jr. H. H. (1998). Taxonomy of Industrially Important White-Rot Fungi, In: Environmentally Friendly Technologies for the Pulp and Paper Industry (eds Young R. A and Akhtar M.). John Wiley Sons. Inc, 259-272.
Cooke, R. C. & Whipps, J. M. (1993). Ecophysiology of fungi. Blackwell Scientific Publications, 85-110.
Deacon, J. W. (1997). Modern Mycology, 3rd edition. Blackwell Science Ltd, 90-103.
Gonzales, T., Terron, M. C., Zapico, E. J., Tellez, A. Yague, S. Carbajo, J. M. & Gonzales, A. E. (2003). Use of Multiplex Reverse Transcription-PCR to Study the Expression of a Laccase Gene Family in a Basidiomycetous Fungus. Applied and Environmental Microbiology, 7083-7090.
https://doi.org/10.1128/AEM.69.12.7083-7090.2003
Hankin, L. & Anagnostakis, S. L. (1977). Solid Media Containing Carboxymethylcellulose to Detect Cx Cellulase Activity of Micro-organuisms. Journal of General Microbiology 98: 109-115.
https://doi.org/10.1099/00221287-98-1-109
Jennings, D. H. & Lysek, G. (1996). Fungal biology: Understanding the fungal lifestyles. United Kingdom: Bios Scientific Publisher Limited. pp. 43-91.
Kasana, R.C., Salwan, R., Dhar, H., Dutt, S. & Gulati, A. (2008). A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram's Iodine. Current Microbiology, 57:503-507.
https://doi.org/10.1007/s00284-008-9276-8
Kirk, T. K. & Cullen, D. (1998). Enzymology and Molecular Genetics of Wood Degradation by White-Rot Fungi In: Environmentally Friendly Technologies for the Pulp and Paper Industry (Young R. A and Akhtar M., eds.), New York : John Wiley & Sons, 273-307.
Koller, G., Moder, M. & Czihal, K. (2000). Peroxidase degradation of selected PCB: a mechanistic study. Chemosphere 41:1827-1834.
https://doi.org/10.1016/S0045-6535(00)00132-6
Maheshwari, R., Bharadwaj, G. & Bhat, M. K. (2000). Thermophilic Fungi: Their Physiology and Enzymes. Journal of Microbiology and Molecular Biology, 64(3): 461-488.
https://doi.org/10.1128/MMBR.64.3.461-488.2000
Mswaka, A.Y. & Magan, N. (1998). Wood degradation, and cellulase and ligninase production by Trametes and other wood-inhibiting Basidiomycetes from indigenous forests of Zimbabwe. Mycological Research, 102 (11): 1399-1404.
https://doi.org/10.1017/S0953756298006789
Obst, J. R., Highley, T. L. & Miller, R. B. (1994). Influence of lignin type on the decay of woody angiosperms by Trametes Versicolor , In: Biodeterioration research 4: Mycotoxins, wood decay, plant stress, biocorrosion, and general biodeterioration (eds Llewellyn G. C, Dashek W. V and O'Rear C. E ).Proceedings of 4th meeting of the Pan American Biodeterioration Society as an electronic symposium. New York: Plenum Press: 357-374.
https://doi.org/10.1007/978-1-4757-9450-2_27
Pataky, N. R. (1999). Wood Rots and Decays. Plant Disease RPD No 642. Department of Crop Sciences of University of Illinois, 1-9.
Roy, A. & De, A. B. (1996). Polyporacea of India. International Book Distributors. India
Smith, D. & Onions, A. H. S. (1994). The preservation and maintenance of living fungi, 2nd edition. International Mycological Institute, CAB International, 15-28.
Tekere, M., Mswaka, A.Y., Zvauya, R. & Read, J. S. (2001a). Growth, dye degradation and ligninolytic activity studies on Zimbabwean white rot fungi. Enzyme and Microbial Technology 28: 420-426.
https://doi.org/10.1016/S0141-0229(00)00343-4
Tekere, M., Zvauya, R. & Read J. S. (2001b). Ligninolytic enzyme production in selected sub-tropical white rot fungi under different culture conditions. Journal of Basic Microbiology, 41(2):115-219.
https://doi.org/10.1002/1521-4028(200105)41:2<115::AID-JOBM115>3.0.CO;2-S
Tomsovsky, M. (2008). Molecular Phylogeny and Taxonomy Position of Trametes cervina and Description of a New Genus Trametopsis. Czech Mycol. 60 (1): 1-11.
https://doi.org/10.33585/cmy.60101
Whiting, E. C., Khan, A. & Gubler, W. D. (2001). Effect of Temperature and Water Potential on Survival and Mycelial Growth of Phaeomoniella chlamydospora and Phaeoacremonium spp. Plant Disease, 85(2): 195-201.
Downloads
How to Cite
Issue
Section
License
Copyright Transfer Statement for Journal
1) In signing this statement, the author(s) grant UNIMAS Publisher an exclusive license to publish their original research papers. The author(s) also grant UNIMAS Publisher permission to reproduce, recreate, translate, extract or summarize, and to distribute and display in any forms, formats, and media. The author(s) can reuse their papers in their future printed work without first requiring permission from UNIMAS Publisher, provided that the author(s) acknowledge and reference publication in the Journal.
2) For open access articles, the author(s) agree that their articles published under UNIMAS Publisher are distributed under the terms of the CC-BY-NC-SA (Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License) which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original work of the author(s) is properly cited.
3) For subscription articles, the author(s) agree that UNIMAS Publisher holds copyright, or an exclusive license to publish. Readers or users may view, download, print, and copy the content, for academic purposes, subject to the following conditions of use: (a) any reuse of materials is subject to permission from UNIMAS Publisher; (b) archived materials may only be used for academic research; (c) archived materials may not be used for commercial purposes, which include but not limited to monetary compensation by means of sale, resale, license, transfer of copyright, loan, etc.; and (d) archived materials may not be re-published in any part, either in print or online.
4) The author(s) is/are responsible to ensure his or her or their submitted work is original and does not infringe any existing copyright, trademark, patent, statutory right, or propriety right of others. Corresponding author(s) has (have) obtained permission from all co-authors prior to submission to the journal. Upon submission of the manuscript, the author(s) agree that no similar work has been or will be submitted or published elsewhere in any language. If submitted manuscript includes materials from others, the authors have obtained the permission from the copyright owners.
5) In signing this statement, the author(s) declare(s) that the researches in which they have conducted are in compliance with the current laws of the respective country and UNIMAS Journal Publication Ethics Policy. Any experimentation or research involving human or the use of animal samples must obtain approval from Human or Animal Ethics Committee in their respective institutions. The author(s) agree and understand that UNIMAS Publisher is not responsible for any compensational claims or failure caused by the author(s) in fulfilling the above-mentioned requirements. The author(s) must accept the responsibility for releasing their materials upon request by Chief Editor or UNIMAS Publisher.
6) The author(s) should have participated sufficiently in the work and ensured the appropriateness of the content of the article. The author(s) should also agree that he or she has no commercial attachments (e.g. patent or license arrangement, equity interest, consultancies, etc.) that might pose any conflict of interest with the submitted manuscript. The author(s) also agree to make any relevant materials and data available upon request by the editor or UNIMAS Publisher.