Diversity of Airborne Fungi at Pepper Plantation Lembah Bidong, Kuala Terengganu

  • NUR AINU FARHAH RABAE Laboratory for Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • SALMAN AZIZ Laboratory for Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • ASAMOAH FREDERICK OSEI Laboratory for Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • SITI NORDAHLIAWATE MOHAMED SIDIQUE Laboratory for Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

Abstract

Piper nigrum L. is well-known as the king of spices and widely used in various field such as food and medicines. In Malaysia, 98% of pepper production comes from the state of Sarawak. The National Commodity Policy (2011-2020) targets to increase the pepper plantation area from the current 16,331 ha to 20,110 ha by year 2020. However, pepper diseases remain as a major challenge in the pepper industry. A great number of airborne fungi pathogen may contribute to a significant economic loss in pepper production. Therefore, this study aims to morphologically identify the diversity of fungi obtained from air-borne samples in a pepper planation that are capable of causing pepper plant diseases. This experiment was conducted at a pepper plantation near Lembah Bidong, Kuala Terengganu. An Andersen spore sampler was used to collect the fungi spores. Culture based identification were then made. The study resulted in the identification of four genus of fungi such as Fusarium sp, Fusarium semictectum Fusarium oxysporum, Curvularia sp., Penicillium sp. and Trichoderma sp. (Ascomycetes). Further molecular identification will confirm the species of fungal pathogens and more understanding of their population as well as severity.

 

Keywords: Pepper, Piper nigrum L., air-borne, fungi, Andersen spore sampler

References

Adam, A., Kho, P. E., Sahari, N., Tida, A., Chen, Y. S., Tawie, & Mohamad, H. (2018). Dr. LADA: Diagnosing black pepper pests and diseases with decision tree. International Journal on Advanced Science, Engineering and Information Technology, 8(4-2), 1584.

https://doi.org/10.18517/ijaseit.8.4-2.6818

Agrios, G. (2005) Plant Pathology. 5th Ed., Elsevier Academic Press, Amsterdam. Akinsanmi, O. & Drenth, A. (2009). Phytophthora diseases management. Australian Macadamia Society Ltd. News Bulletin, 36. 32-34.

Al-Jaradi A., Al-Mahmooli I., Janke R., Maharachchikumbura S., Al-Saady N. & Al-Sadi A.M. (2018). Isolation and identification of pathogenic fungi and oomycetes associated with beans and cowpea root diseases in Oman. PeerJ Journal 6: e6064. DOI: 10.7717/peerj.6064

https://doi.org/10.7717/peerj.6064

Andersen, A.A. (1958) A new sampler for the collection, sizing and enumeration of viable air-borne particles. Journal of Bacteriology, 76(5): 471-484.

https://doi.org/10.1128/jb.76.5.471-484.1958

Brasier C., (2008). Phytophthora Biodiversity: How Many Phytophthora Species Are There? In Goheen, E.M. & Frankel, S.J. (eds) Phytophthoras in Forests and Natural Ecosystems. Proceedings of the Fourth Meeting of the International Union of Forest Research Organizations (IUFRO) Working Party S07.02.09101. August 26-31, 2007, Monterey, California. pp. 101-115

Cai G.H., Hashim J.H., Hashim Z., Ali F., Bloom E., Larsson L., Lampa E. & Norback D. (2011). Fungal DNA, allergens, mycotoxins and associations with asthmatic symptoms among pupils in schools from Johor Bahru, Malaysia. Pediatric Allergy and Immunology, 22: 290-297. DOI: 10.1111/j.1399-3038.2010.01127.x

https://doi.org/10.1111/j.1399-3038.2010.01127.x

Chen, Y.S., Dayod, M., Tawan, C.S. & Science, F. (2010). Phenetic Analysis of Cultivated Black Pepper (Piper nigrum L.) in Malaysia. International Journal of Agronomy, 45(1), 43-47.

Ellis, M.B. (1971). Dematiaceous hypomycetes, commonwealth agricultural bureaux. Slough, England: Farham Royal, p. 440.

Er, C.M., Sunar, N.M., Leman, A.M. & Othman, N. (2015). Direct growth inhibition assay of total airborne fungi with application of biocide-treated malt extract agar. MethodsX, 2: 340-344. DOI: 10.1016/j.mex.2015.07.002

https://doi.org/10.1016/j.mex.2015.07.002

Farhana, S.N.M.D, Rahamah, B.M., Khairulmazmi, A., Wong S.K. & Sariah, M. (2013). Morphological and molecular characterization of Phytophthora capsici, the Causal Agent of foot rot disease of black pepper in Sarawak, Malaysia. International Journal of Agriculture & Biology, 15: 1083-1090.

Farith M.K., Awang, A.S.A.H., Lihan, S. Mohd, H.M. H. & Hairul, A.R. (2015). In vitro antagonism of Phytophthora capsici and Fusarium solani by bacterial isolates from Sarawak. Malaysian Journal of Microbiology, 11(2): 137-143.

Frisvad, J.C., Smedsgaard, J. & Larsen, T.O. (2004). Mycotoxins, drugs and other extrolites produced by species in Penicillium subgenus Penicillium. Studies in Mycology, 49: 201-241.

Gentry, R.F., Mitrovic, M. & Bubash, G.R. (2012). Application of Andersen Sampler in Hatchery Sanitation. Poultry Science, 41(3): 794-804.

https://doi.org/10.3382/ps.0410794

Kifelew, H., Adugna, G. & Tilahun, D. (2017). Reaction of black pepper (Piper nigrum L) accessions against Phytophthora capsici in Ethiopia, proceedings of the fifth biennial conference of Ethiopian Horticultural Science Society (EHSS), Volume V.14-15 February 2015, Samara, Ethiopia.

Ji, F., He, D., Olaniran, A.O., Mokoena, M.P., Xu, J. & Shi, J. (2019). Occurrence, toxicity, production and detection of Fusarium mycotoxin. Food Production, Processing and Nutrition, 1, 6. DOI: 10.1186/s43014-019-0007-2

https://doi.org/10.1186/s43014-019-0007-2

Klich, M. A. (2002). Identification of common Aspergillus species. 1st Edition. Utrech, Netherlands. Centraalbureau voor Schimmelcultures (CBS). Pp. 140.

Kusai, N.A., Mior. Z., Azmi, M., Zulkifly, S., Yusof, M.T. & Mohd Zainudin, N.A.I. (2015). Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rendiconti Lincei, 27(2): 205-214.

https://doi.org/10.1007/s12210-015-0458-6

Lacey, M. & West, J. (2006). The air spora - A manual for catching and identifying airborne biological particles. Dordrecht, the Netherlands. Springer Publishing Company Ltd. DOI: 10.1111/j.1365-3059.2007.01610.x

https://doi.org/10.1111/j.1365-3059.2007.01610.x

Leslie, J.F. & Summerell, B.A. (2006). The Fusarium laboratory manual. Iowa, USA, Blackwell Publishing. Pp. 388.

https://doi.org/10.1002/9780470278376

Liu, T., Liu, L., Jiang, X., Huang, X. & Chen, J. (2010). A new furanoid toxin produced by Curvularia lunata, the causal agent of maize Curvularia leaf spot. Canadian Journal of Plant Pathology, 31 (1): 22-27. DOI: 10.1080/07060660909507568.

https://doi.org/10.1080/07060660909507568

Lucas, J. A. (2020). Plant Pathology and Plant Pathogens, 4th Edition, Wiley-Blackwell. 432.

Magyar, D. & Vass, M. & Li, D.W. (2016). Dispersal Strategies of Microfungi. In Li, D.W. (eds.), Biology of Microfungi, Fungal Biology. Switzerland, Springer International Publishing. DOI 10.1007/978-3-319-29137-6_14.

https://doi.org/10.1007/978-3-319-29137-6_14

Martin, R.R., James D. & Le'vesque, C.A. (2000). Impacts of molecular diagnostic technologies on plant disease management. Annual Review of Phytopathology, 38: 207-239.

https://doi.org/10.1146/annurev.phyto.38.1.207

Shams-Ghahfarokhi, M., Aghaei-Gharehbolagh, S., Aslani, N. & Razzaghi-Abyaneh M. (2014). Investigation on distribution of airborne fungi in outdoor environment in Tehran, Iran. Journal of Environmental Health Science & Engineering, 12, 54.DOI: 10.1186/2052-336X-12-54

https://doi.org/10.1186/2052-336X-12-54

Norbäck, D., Markowicz P., Cai, G-H., Hashim, Z., Ali, F. & Zheng, Y-W. (2014) Endotoxin, Ergosterol, Fungal DNA and Allergens in Dust from Schools in Johor Bahru, Malaysia- Associations with Asthma and Respiratory Infections in Pupils. PLoS ONE, 9, 2. DOI: 10.1371/journal.pone.0088303

https://doi.org/10.1371/journal.pone.0088303

Pady, S.M., & Kapica, L. (2007). Fungi in Air over the Atlantic Ocean. Mycologia, 47(1): 34-50. DOI: 10.2307/3755754

https://doi.org/10.2307/3755754

Perrone, G. & Susca, A. (2017). Penicillium Species and their associated mycotoxins. In Moretti, A. and Susca, A. (eds) Mycotoxigenic Fungi: Methods in molecular biology, Vol 1542. New York, Humana Press.

https://doi.org/10.1007/978-1-4939-6707-0_5

Rajan P, Sarma Y.R. & Anandaraj, M. (2002). Management of foot rot disease of black pepper with Trichoderma spp. Indian Phytopath, 55 (1): 34-38.

Rivka, B.G., (2001). Chapter 5 - Attack Mechanisms of the Pathogen, Postharvest Diseases of Fruits and Vegetables. Elsevier, Amsterdam, pp. 54-65.

https://doi.org/10.1016/B978-044450584-2/50005-8

Shahnazi, S., Meon, S., Vadamalai, G.K. Ahmad, K. & Nejat, N. (2012). Morphological and molecular characterization of Fusarium spp. associated with yellowing disease of black pepper (Piper nigrum L.) in Malaysia. Journal of General Plant Pathology, 78 (3): 160-169. DOI: 10.1007/s10327-012-0379-5

https://doi.org/10.1007/s10327-012-0379-5

Siti Nordahliawate M.S., Yong-Ju H., Avice, M.H. & Bruce D.L. F. (2012). Maturation of Leptosphaeria maculans and L. biglobosa pseudothecia and first appearance of phoma leaf spots on winter oilseed rape. Aspects of Applied Biology, 117: 209-215.

Siti Nordahliawate, M.S., Nur Ain, I.M.Z., Nur Azlin, A. & Baharuddin, S. (2012). Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia. Pertanika Journal of Tropical Agricultural and Sciences, 35 (2): 381-386.

Spellerberg, I.F. (2008). Encyclopedia of Ecology (p.3249-3252). New Zealand: Lincoln University, Lincoln.

https://doi.org/10.1016/B978-008045405-4.00132-4

Thomas, L. M. (2017). Survey for the Incidence of Foot Rot of Black Pepper Caused by Phytophthora capsici Leonian in Shivamogga and Chickmagaluru Districts of Karnataka State. International Journal of Pure & Applied Bioscience, 5(1): 293-298.

https://doi.org/10.18782/2320-7051.2562

Watanabe, T. (2002). Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. Boca Raton, CRC Press. DOI: 10.1201/9781420040821

https://doi.org/10.1201/9781420040821

West, J. S., & Kimber, R.B.E. (2015). Innovations in air sampling to detect plant pathogens. Annals of Applied Biology, 166(1): 4-17.

https://doi.org/10.1111/aab.12191

Wyatt, T.T., Wösten, H.A.B., & Dijksterhuis, J. (2013). Fungal spores for dispersion in space and time. In Sariaslani, S. & Gadd G.M. (eds) Advances in Applied Microbiology, 85: 43-91. DOI: 10.1016/B978-0-12-407672-3.00002-2

https://doi.org/10.1016/B978-0-12-407672-3.00002-2

Xiong,W., Li, Z., Liu, H., Xue, C., Zhang, R. & Wu, H. (2015) The effect of long term continuous cropping of black pepper on soil bacterial communities as determined by 454 pyrosequencing. PLoS ONE, 10, 8.DOI: 10.1371/journal.pone.0136946

https://doi.org/10.1371/journal.pone.0136946

Zhang, M., Zhang, T.Y. & Wu, W.P. (2004). A new name and a new variety in Curvularia. Mycosystema, 23, 177-178.

Published
2020-12-31
How to Cite
NUR AINU FARHAH RABAE, SALMAN AZIZ, ASAMOAH FREDERICK OSEI, & SITI NORDAHLIAWATE MOHAMED SIDIQUE. (2020). Diversity of Airborne Fungi at Pepper Plantation Lembah Bidong, Kuala Terengganu. Borneo Journal of Resource Science and Technology, 10(2), 147-154. https://doi.org/10.33736/bjrst.2683.2020