Diversity of Airborne Fungi at Pepper Plantation Lembah Bidong, Kuala Terengganu
DOI:
https://doi.org/10.33736/bjrst.2683.2020Abstract
Piper nigrum L. is well-known as the king of spices and widely used in various field such as food and medicines. In Malaysia, 98% of pepper production comes from the state of Sarawak. The National Commodity Policy (2011-2020) targets to increase the pepper plantation area from the current 16,331 ha to 20,110 ha by year 2020. However, pepper diseases remain as a major challenge in the pepper industry. A great number of airborne fungi pathogen may contribute to a significant economic loss in pepper production. Therefore, this study aims to morphologically identify the diversity of fungi obtained from air-borne samples in a pepper planation that are capable of causing pepper plant diseases. This experiment was conducted at a pepper plantation near Lembah Bidong, Kuala Terengganu. An Andersen spore sampler was used to collect the fungi spores. Culture based identification were then made. The study resulted in the identification of four genus of fungi such as Fusarium sp, Fusarium semictectum Fusarium oxysporum, Curvularia sp., Penicillium sp. and Trichoderma sp. (Ascomycetes). Further molecular identification will confirm the species of fungal pathogens and more understanding of their population as well as severity.
Keywords: Pepper, Piper nigrum L., air-borne, fungi, Andersen spore sampler
References
Adam, A., Kho, P. E., Sahari, N., Tida, A., Chen, Y. S., Tawie, & Mohamad, H. (2018). Dr. LADA: Diagnosing black pepper pests and diseases with decision tree. International Journal on Advanced Science, Engineering and Information Technology, 8(4-2), 1584.
https://doi.org/10.18517/ijaseit.8.4-2.6818
Agrios, G. (2005) Plant Pathology. 5th Ed., Elsevier Academic Press, Amsterdam. Akinsanmi, O. & Drenth, A. (2009). Phytophthora diseases management. Australian Macadamia Society Ltd. News Bulletin, 36. 32-34.
Al-Jaradi A., Al-Mahmooli I., Janke R., Maharachchikumbura S., Al-Saady N. & Al-Sadi A.M. (2018). Isolation and identification of pathogenic fungi and oomycetes associated with beans and cowpea root diseases in Oman. PeerJ Journal 6: e6064. DOI: 10.7717/peerj.6064
https://doi.org/10.7717/peerj.6064
Andersen, A.A. (1958) A new sampler for the collection, sizing and enumeration of viable air-borne particles. Journal of Bacteriology, 76(5): 471-484.
https://doi.org/10.1128/jb.76.5.471-484.1958
Brasier C., (2008). Phytophthora Biodiversity: How Many Phytophthora Species Are There? In Goheen, E.M. & Frankel, S.J. (eds) Phytophthoras in Forests and Natural Ecosystems. Proceedings of the Fourth Meeting of the International Union of Forest Research Organizations (IUFRO) Working Party S07.02.09101. August 26-31, 2007, Monterey, California. pp. 101-115
Cai G.H., Hashim J.H., Hashim Z., Ali F., Bloom E., Larsson L., Lampa E. & Norback D. (2011). Fungal DNA, allergens, mycotoxins and associations with asthmatic symptoms among pupils in schools from Johor Bahru, Malaysia. Pediatric Allergy and Immunology, 22: 290-297. DOI: 10.1111/j.1399-3038.2010.01127.x
https://doi.org/10.1111/j.1399-3038.2010.01127.x
Chen, Y.S., Dayod, M., Tawan, C.S. & Science, F. (2010). Phenetic Analysis of Cultivated Black Pepper (Piper nigrum L.) in Malaysia. International Journal of Agronomy, 45(1), 43-47.
Ellis, M.B. (1971). Dematiaceous hypomycetes, commonwealth agricultural bureaux. Slough, England: Farham Royal, p. 440.
Er, C.M., Sunar, N.M., Leman, A.M. & Othman, N. (2015). Direct growth inhibition assay of total airborne fungi with application of biocide-treated malt extract agar. MethodsX, 2: 340-344. DOI: 10.1016/j.mex.2015.07.002
https://doi.org/10.1016/j.mex.2015.07.002
Farhana, S.N.M.D, Rahamah, B.M., Khairulmazmi, A., Wong S.K. & Sariah, M. (2013). Morphological and molecular characterization of Phytophthora capsici, the Causal Agent of foot rot disease of black pepper in Sarawak, Malaysia. International Journal of Agriculture & Biology, 15: 1083-1090.
Farith M.K., Awang, A.S.A.H., Lihan, S. Mohd, H.M. H. & Hairul, A.R. (2015). In vitro antagonism of Phytophthora capsici and Fusarium solani by bacterial isolates from Sarawak. Malaysian Journal of Microbiology, 11(2): 137-143.
Frisvad, J.C., Smedsgaard, J. & Larsen, T.O. (2004). Mycotoxins, drugs and other extrolites produced by species in Penicillium subgenus Penicillium. Studies in Mycology, 49: 201-241.
Gentry, R.F., Mitrovic, M. & Bubash, G.R. (2012). Application of Andersen Sampler in Hatchery Sanitation. Poultry Science, 41(3): 794-804.
https://doi.org/10.3382/ps.0410794
Kifelew, H., Adugna, G. & Tilahun, D. (2017). Reaction of black pepper (Piper nigrum L) accessions against Phytophthora capsici in Ethiopia, proceedings of the fifth biennial conference of Ethiopian Horticultural Science Society (EHSS), Volume V.14-15 February 2015, Samara, Ethiopia.
Ji, F., He, D., Olaniran, A.O., Mokoena, M.P., Xu, J. & Shi, J. (2019). Occurrence, toxicity, production and detection of Fusarium mycotoxin. Food Production, Processing and Nutrition, 1, 6. DOI: 10.1186/s43014-019-0007-2
https://doi.org/10.1186/s43014-019-0007-2
Klich, M. A. (2002). Identification of common Aspergillus species. 1st Edition. Utrech, Netherlands. Centraalbureau voor Schimmelcultures (CBS). Pp. 140.
Kusai, N.A., Mior. Z., Azmi, M., Zulkifly, S., Yusof, M.T. & Mohd Zainudin, N.A.I. (2015). Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rendiconti Lincei, 27(2): 205-214.
https://doi.org/10.1007/s12210-015-0458-6
Lacey, M. & West, J. (2006). The air spora - A manual for catching and identifying airborne biological particles. Dordrecht, the Netherlands. Springer Publishing Company Ltd. DOI: 10.1111/j.1365-3059.2007.01610.x
https://doi.org/10.1111/j.1365-3059.2007.01610.x
Leslie, J.F. & Summerell, B.A. (2006). The Fusarium laboratory manual. Iowa, USA, Blackwell Publishing. Pp. 388.
https://doi.org/10.1002/9780470278376
Liu, T., Liu, L., Jiang, X., Huang, X. & Chen, J. (2010). A new furanoid toxin produced by Curvularia lunata, the causal agent of maize Curvularia leaf spot. Canadian Journal of Plant Pathology, 31 (1): 22-27. DOI: 10.1080/07060660909507568.
https://doi.org/10.1080/07060660909507568
Lucas, J. A. (2020). Plant Pathology and Plant Pathogens, 4th Edition, Wiley-Blackwell. 432.
Magyar, D. & Vass, M. & Li, D.W. (2016). Dispersal Strategies of Microfungi. In Li, D.W. (eds.), Biology of Microfungi, Fungal Biology. Switzerland, Springer International Publishing. DOI 10.1007/978-3-319-29137-6_14.
https://doi.org/10.1007/978-3-319-29137-6_14
Martin, R.R., James D. & Le'vesque, C.A. (2000). Impacts of molecular diagnostic technologies on plant disease management. Annual Review of Phytopathology, 38: 207-239.
https://doi.org/10.1146/annurev.phyto.38.1.207
Shams-Ghahfarokhi, M., Aghaei-Gharehbolagh, S., Aslani, N. & Razzaghi-Abyaneh M. (2014). Investigation on distribution of airborne fungi in outdoor environment in Tehran, Iran. Journal of Environmental Health Science & Engineering, 12, 54.DOI: 10.1186/2052-336X-12-54
https://doi.org/10.1186/2052-336X-12-54
Norbäck, D., Markowicz P., Cai, G-H., Hashim, Z., Ali, F. & Zheng, Y-W. (2014) Endotoxin, Ergosterol, Fungal DNA and Allergens in Dust from Schools in Johor Bahru, Malaysia- Associations with Asthma and Respiratory Infections in Pupils. PLoS ONE, 9, 2. DOI: 10.1371/journal.pone.0088303
https://doi.org/10.1371/journal.pone.0088303
Pady, S.M., & Kapica, L. (2007). Fungi in Air over the Atlantic Ocean. Mycologia, 47(1): 34-50. DOI: 10.2307/3755754
https://doi.org/10.2307/3755754
Perrone, G. & Susca, A. (2017). Penicillium Species and their associated mycotoxins. In Moretti, A. and Susca, A. (eds) Mycotoxigenic Fungi: Methods in molecular biology, Vol 1542. New York, Humana Press.
https://doi.org/10.1007/978-1-4939-6707-0_5
Rajan P, Sarma Y.R. & Anandaraj, M. (2002). Management of foot rot disease of black pepper with Trichoderma spp. Indian Phytopath, 55 (1): 34-38.
Rivka, B.G., (2001). Chapter 5 - Attack Mechanisms of the Pathogen, Postharvest Diseases of Fruits and Vegetables. Elsevier, Amsterdam, pp. 54-65.
https://doi.org/10.1016/B978-044450584-2/50005-8
Shahnazi, S., Meon, S., Vadamalai, G.K. Ahmad, K. & Nejat, N. (2012). Morphological and molecular characterization of Fusarium spp. associated with yellowing disease of black pepper (Piper nigrum L.) in Malaysia. Journal of General Plant Pathology, 78 (3): 160-169. DOI: 10.1007/s10327-012-0379-5
https://doi.org/10.1007/s10327-012-0379-5
Siti Nordahliawate M.S., Yong-Ju H., Avice, M.H. & Bruce D.L. F. (2012). Maturation of Leptosphaeria maculans and L. biglobosa pseudothecia and first appearance of phoma leaf spots on winter oilseed rape. Aspects of Applied Biology, 117: 209-215.
Siti Nordahliawate, M.S., Nur Ain, I.M.Z., Nur Azlin, A. & Baharuddin, S. (2012). Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia. Pertanika Journal of Tropical Agricultural and Sciences, 35 (2): 381-386.
Spellerberg, I.F. (2008). Encyclopedia of Ecology (p.3249-3252). New Zealand: Lincoln University, Lincoln.
https://doi.org/10.1016/B978-008045405-4.00132-4
Thomas, L. M. (2017). Survey for the Incidence of Foot Rot of Black Pepper Caused by Phytophthora capsici Leonian in Shivamogga and Chickmagaluru Districts of Karnataka State. International Journal of Pure & Applied Bioscience, 5(1): 293-298.
https://doi.org/10.18782/2320-7051.2562
Watanabe, T. (2002). Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. Boca Raton, CRC Press. DOI: 10.1201/9781420040821
https://doi.org/10.1201/9781420040821
West, J. S., & Kimber, R.B.E. (2015). Innovations in air sampling to detect plant pathogens. Annals of Applied Biology, 166(1): 4-17.
https://doi.org/10.1111/aab.12191
Wyatt, T.T., Wösten, H.A.B., & Dijksterhuis, J. (2013). Fungal spores for dispersion in space and time. In Sariaslani, S. & Gadd G.M. (eds) Advances in Applied Microbiology, 85: 43-91. DOI: 10.1016/B978-0-12-407672-3.00002-2
https://doi.org/10.1016/B978-0-12-407672-3.00002-2
Xiong,W., Li, Z., Liu, H., Xue, C., Zhang, R. & Wu, H. (2015) The effect of long term continuous cropping of black pepper on soil bacterial communities as determined by 454 pyrosequencing. PLoS ONE, 10, 8.DOI: 10.1371/journal.pone.0136946
https://doi.org/10.1371/journal.pone.0136946
Zhang, M., Zhang, T.Y. & Wu, W.P. (2004). A new name and a new variety in Curvularia. Mycosystema, 23, 177-178.
Downloads
Published
How to Cite
Issue
Section
License
Copyright Transfer Statement for Journal
1) In signing this statement, the author(s) grant UNIMAS Publisher an exclusive license to publish their original research papers. The author(s) also grant UNIMAS Publisher permission to reproduce, recreate, translate, extract or summarize, and to distribute and display in any forms, formats, and media. The author(s) can reuse their papers in their future printed work without first requiring permission from UNIMAS Publisher, provided that the author(s) acknowledge and reference publication in the Journal.
2) For open access articles, the author(s) agree that their articles published under UNIMAS Publisher are distributed under the terms of the CC-BY-NC-SA (Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License) which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original work of the author(s) is properly cited.
3) For subscription articles, the author(s) agree that UNIMAS Publisher holds copyright, or an exclusive license to publish. Readers or users may view, download, print, and copy the content, for academic purposes, subject to the following conditions of use: (a) any reuse of materials is subject to permission from UNIMAS Publisher; (b) archived materials may only be used for academic research; (c) archived materials may not be used for commercial purposes, which include but not limited to monetary compensation by means of sale, resale, license, transfer of copyright, loan, etc.; and (d) archived materials may not be re-published in any part, either in print or online.
4) The author(s) is/are responsible to ensure his or her or their submitted work is original and does not infringe any existing copyright, trademark, patent, statutory right, or propriety right of others. Corresponding author(s) has (have) obtained permission from all co-authors prior to submission to the journal. Upon submission of the manuscript, the author(s) agree that no similar work has been or will be submitted or published elsewhere in any language. If submitted manuscript includes materials from others, the authors have obtained the permission from the copyright owners.
5) In signing this statement, the author(s) declare(s) that the researches in which they have conducted are in compliance with the current laws of the respective country and UNIMAS Journal Publication Ethics Policy. Any experimentation or research involving human or the use of animal samples must obtain approval from Human or Animal Ethics Committee in their respective institutions. The author(s) agree and understand that UNIMAS Publisher is not responsible for any compensational claims or failure caused by the author(s) in fulfilling the above-mentioned requirements. The author(s) must accept the responsibility for releasing their materials upon request by Chief Editor or UNIMAS Publisher.
6) The author(s) should have participated sufficiently in the work and ensured the appropriateness of the content of the article. The author(s) should also agree that he or she has no commercial attachments (e.g. patent or license arrangement, equity interest, consultancies, etc.) that might pose any conflict of interest with the submitted manuscript. The author(s) also agree to make any relevant materials and data available upon request by the editor or UNIMAS Publisher.