A PRELIMINARY STUDY ON THE POLYMORPHISM OF HYPERVARIABLE D17S5 LOCUS IN UNIMAS CHINESE POPULATION

  • Hairul Azman Roslan
  • Jessie Ann Sim Chiew Ling
  • Rosmawati Saat
Keywords: VNTR, D17S5, polymerase chain reaction, forensic identification, allele distribution

Abstract

Variable number of tandem repeats (VNTRs) is one of the most useful tools in the study of polymorphism and have spurred studies in many populations throughout the world. Here we report a preliminary VNTR study of the polymorphism of the hypervariable locus D17S5, in the Chinese community in UNIMAS. Samples were obtained from seventy-five unrelated Chinese individuals. The study showed the presence of six alleles ranging from 170bp to 520bp in sizes. The allele frequencies ranged from 0.02 to 0.44 with heterozygosity value of 0.32. The distributions of the D17S5 alleles in the UNIMAS Chinese population were also determined with allele 2 having the highest frequency and alleles 5 and 6 being the lowest frequencies, respectively.

References

Carr, S. M. (2004). Principles of human population genetics. Memorial University of Newfoundland Website: http://www.mun.ca/biology/scarr/6390_ Population_Genetics.html

Gasparini, P., Mandich, P., Novelli, G., Bellone, E., Sangiuolo, F., De Stefano, F., Potenza, L., Trabetti, E., Marigo, M., Pignatti, P. F., Dallapiccola, B. & Ajmar F. (1991). Forensic applications of molecular genetic analysis: An Italian collaborative study on paternity testing by the determination of variable number of tandem repeat DNA polymorphisms. Human Heredity, 41:174-181.

https://doi.org/10.1159/000153997

Hartl, D. L. & Clark, A. G. (1997). Principles of Population Genetics (3rd Ed.). Sinauer.

Hoong, L. L. & Lek, K. C. 2005. Genetic polymorphisms in mitochondrial DNA hypervariable regions I, II and III of the Malaysian population. Asia Pacific Journal of Molecular Biology and Biotechnology 13:2 79-85.

Horn, G. T., Richards, B. & Klinger, K. W. (1989). Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nucleic Acids Research 17(5): 2140.

https://doi.org/10.1093/nar/17.5.2140

Katsuyama, Y., Inoko, H., Imanishi, T., Mizuki, N., Gojobori, T. & Ota, M. (1998). Genetic relationships among Japanese, Northern Han, Hui, Uygur, Kazakh, Greek, Saudi Arabian, and Italian populations based on allelic frequencies at four VNTR (D1S80, D4S43, COL2A1, D17S5) and one STR (ACTBP2) loci. Human Heredity, 48: 126-137.

https://doi.org/10.1159/000022793

Kijas, J. M. H., Fowler, J. C. S. & Van Daal, A. (1994). PCR amplification of alleles at locus D17S5: Detection of new and rare long-length alleles by oligoprobing in a survey of Australian populations. Website http://www.findarticles.com/p/articles/mi_qa3659/is_199404/ai_n8729501.

Pinheiro, M. F., Pontes, M. L., Huguet, E., Gene, M., Pinto da Costa, J. & Moreno, P. (1996). Study of three AMPFLPs (D1S80, 3'ApoB and YNZ22) in the population of the North of Portugal. Forensic Science International, 79: 23-29.

https://doi.org/10.1016/0379-0738(95)01883-2

Richards, B., Skoletsky, J., Shuber, P. A., Balfour, R., Stern, R. C., Dorkin, H. L., Parad, R. B., Witt, D. & Klinger, K. W. (1993). Multiplex PCR amplification from the CRFT gene using DNA prepared from buccal brushes/swabs. Human Molecular Genetics, 2: 159-163.

https://doi.org/10.1093/hmg/2.2.159

Sulaiman, N. S. (2006). Allele frequencies distribution of hypervariable loci in Melanau population sample of UNIMAS. Final Year Project, Universiti Malaysia Sarawak, Kota Samarahan. Unpublished.

Tamaki, K. & Jeffreys, A. J. (2005). Human tandem repeat sequences in forensic DNA typing. Journal of Legal Medicine, 7(4): 244-250.

https://doi.org/10.1016/j.legalmed.2005.02.002

Yang, Y. S., Wong, L. P., Lee, T. C., Mustafa, A. M., Mohamed, Z. & Lang C. (2004). Genetic polymorphism of cytochrome P450 2C19 in healthy Malaysian subjects. British Journal of Clinical Pharmacology 58:3 332-335.

https://doi.org/10.1111/j.1365-2125.2004.02144.x

Zainuddin, Z., Teh, L. K., Suhaimi, A. W. M. & Ismail, R. (2006). Malaysian Indians are genetically similar to Caucasians: CYP2C9 polymorphism. Journal of Clinical Pharmacy and Therapeutics 31:2 187-191.

https://doi.org/10.1111/j.1365-2710.2006.00727.x

Published
2016-06-29
How to Cite
Roslan, H. A., Chiew Ling, J. A. S., & Saat, R. (2016). A PRELIMINARY STUDY ON THE POLYMORPHISM OF HYPERVARIABLE D17S5 LOCUS IN UNIMAS CHINESE POPULATION. Borneo Journal of Resource Science and Technology, 1(1), 24-27. https://doi.org/10.33736/bjrst.259.2011
Section
General