The Control of Panicle Blight Bacterial Pathogen on Rice Seeds Through In Vitro Treatments

Authors

  • IRDA SAFNI Universitas Sumatera Utara
  • UCI UTARI Universitas Sumatera Utara
  • MARYANI CYCCU TOBING Universitas Sumatera Utara
  • LISNAWITA Universitas Sumatera Utara

DOI:

https://doi.org/10.33736/bjrst.2542.2020

Abstract

Burkholderia glumae is a seed-borne pathogen of rice known to cause bacterial panicle blight disease. The lack of effective control methods makes seed treatment the alternative management approach. The aim of this research was to determine an effective seed treatments technique, using liquid smoke, clove oil, hot water and copper hydroxide fungicide treatment against bacteria B. glumae. The experiment used a complete randomized design with five treatments and three replications, including control, liquid smoke, clove oil, hot water, and copper hydroxide fungicide. The results showed the propensity for all treatments to reduce bacterial populations on rice seeds, while liquid smoke, clove oil, and fungicide did not reduce vigour and viability. Application of copper hydroxide fungicide 77% at concentration of 5% was recommended as the best treatment to control the bacterial pathogen.

Keywords: Burkholderia glumae, clove oil, copper hydroxide fungicide, hot water, liquid smoke, rice

References

Abdollahi, M.R. (2012). Effect of plant-derived smoke on germination, seedling vigour and growth of rapeseed (Brassica napus) under laboratory and greenhouse conditions. Seed Science & Technology, 40(3): 437-442

https://doi.org/10.15258/sst.2012.40.3.15

Achrom, M. (2015). Perlakuan pencelupan asap cair untuk mengeleminasi bakteri Burkholderia glumae Kurita & Tabei pada benih padi (Oryza sativa L.)

[Dipping treatment of liquid smoke to eliminate bacteri Burkholderia glumae Kurita and Tabei on rice (Oryza sativa L)]. Balai Uji Terap Teknik dan Metode Karantina Pertanian, Jawa Barat, Indonesia.

Aisyah, I., Sinaga, M.S., Nawangsih, A.S., Giyanto & Pari G. (2018). Utilization of liquid smoke to suppress blood diseases on bananas and its effects on the plant growth. AGRIVITA Journal of Agricultural Sciences, 40(3): 453-460.

https://doi.org/10.17503/agrivita.v40i3.1390

Baharuddin, Harniati R., Faisal, F., Yani, A., Suparmi, Hamid, H., Kuswinanti, T. & Jahuddin R. (2017). Incidence of panicle blight disease (Burkholderia glumae) on rice in South Sulawesi. [Keberadaan penyakit busuk bulir (Burkholderia glumae) pada tanaman padi di Sulawesi Selatan]. In Pradana,M.G. & Mubin, N. (eds.). Proceedings Simposium Nasional Fitopatologi Indonesia, Bogor, Indonesia.

Belmar, S.B., Kelsey, C.D., Gebremariam, T.A., Wamishe, Y.A. (2014). Seed management to control bacterial panicle blight of rice. AAES Research Series, 62: 82-94.

Directorate General of Food Crop - Directorate of Rice and Holticultural Production Sub Directorate of Quality Control and Seed Certification. 1991. Petunjuk Pengawas Benih [Directory of Seed Control]. Jakarta.

Flematti, G.R., Merritt, D.J., Piggott, M.J., Trengove, R.D., Smith, S.M., Dixon, K.W. & Ghisalberti, E.L. (2011). Burning vegetation produces cyanohydrins that liberate cyanide and stimulate seed germination. Nature Communications, 2: 360. DOI: 10.1038 /ncomms1356

https://doi.org/10.1038/ncomms1356

Hasanah, Y. (2017). Karakterisasi dan Pengendalian Burkholderia glumae Penyebab Penyakit Bacterial Grain Rot Menggunakan Teknologi Perlakuan Benih Kombinasi Perlakuan Fisik dan Kimia [Characterization and Control of Burkholderia glumae the Causal Pathogen of Bacterial Grain Rot Using Technology of Seed Treatment with Combination of Physichal and Chemical Treatments]. (Thesis), Universitas Brawijaya, Indonesia.

Hasibuan, M., Safni, I., Lisnawita & Lubis, K. (2018). Morphological characterization of several strainsof the rice pathogenic bacterium Burkholderia glumae in North Sumatra. IOP Conf. Series:Earth and Environmental Science, 122: 012044.

https://doi.org/10.1088/1755-1315/122/1/012044

Hikichi, Y. & Egami, H. (1998). Control systems for bacterial grain rot of rice with oxolinic acid and seed selection with salt solution. Annals of the Phytopathological Society of Japan, 61(4): 405-409.

https://doi.org/10.3186/jjphytopath.61.405

Hoerussalam., Roesli, E.Z., Suparto, A. & Lukman, R. (2017). Cara Efektif Untuk Menghilangkan Burkholderia glumae Dan Meningkatkan Kualitas Benih Padi. Seminar Nasional Kongres XXIV Perhimpunan Fitopatologi Indonesia, Kendari, Indonesia.

Ishaq, M., Rumiati, A.T., Permatasari, E.O. (2017). Analisis faktor - faktor yang mempengaruhi produksi padi di Provinsi Jawa Timur menggunakan regresi semiparametrik spline [Analysis factors effecting rice production in East Java using regression semiparametric spline]. Jurnal Sains dan Seni, 6(1): 2337-3520.

https://doi.org/10.12962/j23373520.v6i1.22451

Kishore, G. K., Pande, S. & Harish, S. (2007). Evaluation of essential oils and their components for broad-spectrum antifungal activity and control of late leaf spot and crown rot diseases in peanut. Plant Disease, 91: 375-379

https://doi.org/10.1094/PDIS-91-4-0375

Lamichhane, J.R., Barzman, L., Booij, K., Boonekamp, P., Desneux, N., Huber, L., Kudsk, P., Langrell, S.E.H., Ratnadass, A., Ricci, P., Sarah, J.L. & Messéan, A. (2015). Robust cropping systems to

tackle pests under climate change. A review. Agronomy for Sustainable Development, 35(2): 443-459.

Light, M.E. & van Staden, J. (2004). The potential of smoke in seed technology. South African Journal of Botany, 70(1): 97-101.

https://doi.org/10.1016/S0254-6299(15)30311-2

Lingbeck, J.M., Cordero, P., O'Bryan, C.A., Johnson, M.G., Ricke, S.C. & Crandall, P.G. (2014). Functionality of liquid smoke as an all-natural antimicrobial in food preservation. Meat Science, 97(2): 197-206.

https://doi.org/10.1016/j.meatsci.2014.02.003

Milus, E.A. & Rothrock, C.S. (1997). Efficacy of bacterial seed treatments for controlling Pythium root rot of winter wheat. Plant Disease, 81: 180-184.

https://doi.org/10.1094/PDIS.1997.81.2.180

Nalis, S. (2015). Keefektifan perlakuan microwave, air panas, panas kering, dan bakterisida untuk menekan infeksi Pantoea stewartii subsp. stewartii pada benih jagung manis. [The efectivity of microwave, hot water, hot dry and bactericide to supress infection of Pantoea stewartii subsp. stewartii on sweetcorn seeds]. (Thesis), Institut Pertanian Bogor, Indonesia.

https://doi.org/10.14692/jfi.11.4.128

Payamara, J. (2011). Usage of wood vinegar as new organic substance. International Journal of ChemTech Research, 3(3): 1658-1662.

Quarantine Agriculture. (2015). Lampiran Keputusan Menteri Pertanian (Permentan) No 51/Permentan/KR010/9/2015 tentang Jenis-jenis Organisme Pengganggu Tumbuhan Karantina (OPTK) Kategori A1 dan A2. [Appendices of Ministry of Agriculture Injunction No 51/Permentan/KR010/9/2015 about different quarantine plant pests category A1 and A2]

Situmeang, M. (2013). Pengaruh Berbagai Perlakuan Untuk Mengendalikan Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) Terbawa Benih Serta Meningkatkan Viabilitas dan Vigor Benih Padi (Oryza sativa L.). [Effect of Several Treatments to Control Seed-borne Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) and to Improve Seed Vigor and Viability (Oryza sativa L). (Thesis), University of Gadjah Mada, Yogyakarta, Indonesia.

Spadaro, D., Herforth-Rahme, J. & van der Wolf, J. (2017). Organic seed treatments of vegetables to prevent seedborne diseases. Acta Horticulture, 1164, 23-32. DOI: 10.17660/ActaHortic.2017.1164.3

https://doi.org/10.17660/ActaHortic.2017.1164.3

Suryani L. (2017): Burkholderia glumae Penyebab Hawar pada Malai Padi. [Burkholderia glumae the causal pathogen of rice leaf blight]. Department of Agricultural Quarantine Class 1, Banjarmasin, Indonesia.

Sutopo L. (2004): Teknologi Benih. [Seed Technology]. Rajawali Press, Jakarta, Indonesia.

Tapke, VF. (1923). Effects of the modified hot water treatment on germination, growth and yield of wheat. Journal of Agricultural Research, 28(1): 79-97.

Taylor, J.D. & Dye, D.W. (1976), Evaluation of streptomycin seed treatments for the control of bacterial blight of peas (Pseudomonas pisi Sackett 1916). New Zealand. Journal of Agricultural Research, 19(1): 91-95. DOI: 10.1080/ 00288233.1976.10421050

https://doi.org/10.1080/00288233.1976.10421050

Trung, H.M., Van, N.V., Vien, N.V., Lam, D.T. & Lien, M. (1993). Occurrence of Rice Grain Rot Disease in Vietnam. International Rice Research Notes, 18(3): 30.

Tung, L.D. & Serrano, E.P. (2011). Effects of warm in breaking dormancy of rice seed. Omonrice, 18: 129-136. Urakami, T., Ito-Yoshida, C., Araki, H., Kijima, T., Suzuki, K. I., & Komagata, K. (1994). Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and Description of Burkholderia vandii sp. International Journal of Systematic and Evolutionary Microbiology, 44(2): 235-245.

https://doi.org/10.1099/00207713-44-2-235

van der Wolf, J.M., Bimbaum, Y., van der Zouwen, P.S. & Groot, S.P.C. (2008). Disinfection of vegetable seed by treatment with essential oils, organic acids and plant extracts. Seed Science and Technolongy, 36: 76-88.

https://doi.org/10.15258/sst.2008.36.1.08

Wang, M., Schuettner, M., Xu, S., Paetz, C., Wilder, J., Baldwin, I.T. & Groten, K. (2017). Catechol, a major component of smoke, influences primary root growth and root hair elongation through reactive oxygen. New Phytologist, 213: 1755-1770.

https://doi.org/10.1111/nph.14317

Wiyono, S., Giyanto, Mutaqin, K.H., Hidayat, S.H. & Supramana, W. (2017). Emerging Diseases pada Tanaman Pertanian: strategi dan opsi kebijakan pengendalian. In Pradana, M.G. & Mubin, N. (eds.). Simposium Nasional Fitopatologi Bogor, Indonesia.

Yang, J.F., Yang, C.H., Liang, M.T., Gao, Z.J., Wu, Y.W. & Chuang, L.Y. (2016). Chemical composition, antioxidant, and antibacterial activity of wood vinegar from Litchi chinensis. Molecules, 21(9): 1150. DOI: 10.3390/molecules21091150

https://doi.org/10.3390/molecules21091150

Zagory, D., Parmeter, J.R., Jr. (1984). Fungitoxicity of smoke. Phytopathology, 74: 1027-1031.

https://doi.org/10.1094/Phyto-74-1027

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Published

2020-12-31

How to Cite

SAFNI, I., UTARI, U., TOBING , M. C. ., & LISNAWITA. (2020). The Control of Panicle Blight Bacterial Pathogen on Rice Seeds Through In Vitro Treatments. Borneo Journal of Resource Science and Technology, 10(2), 141–146. https://doi.org/10.33736/bjrst.2542.2020