Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
DOI:
https://doi.org/10.33736/bjrst.248.2013Keywords:
Bacillus amyliquefaciens, starch degrading, amylase, in silico, sago wasteAbstract
Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, acellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the
α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino
acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528).
The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B.
subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed
similar characteristics compared to α-amylase from B. subtilis X-23.
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