Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002

Authors

  • Muhammad Suhaib Mat Hussin
  • Mohd Hasnain Hussain
  • Awang Ahmad Sallehin Awang Husaini
  • Koplo Bujang
  • Dayang Salwani Awg Adeni
  • Mohd Azib Salleh

DOI:

https://doi.org/10.33736/bjrst.248.2013

Keywords:

Bacillus amyliquefaciens, starch degrading, amylase, in silico, sago waste

Abstract

Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a
cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the
α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino
acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528).
The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B.
subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed
similar characteristics compared to α-amylase from B. subtilis X-23.

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How to Cite

Mat Hussin, M. S., Hussain, M. H., Sallehin Awang Husaini, A. A., Bujang, K., Awg Adeni, D. S., & Salleh, M. A. (2016). Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002. Borneo Journal of Resource Science and Technology, 3(2), 36–39. https://doi.org/10.33736/bjrst.248.2013

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