Prevalence of Leptospira Species in Environmental Soil and Water from National Parks in Sarawak, Malaysia

Authors

  • Chai Fung Pui
  • Lesley Maurice Bilung
  • Lela Su'ut
  • Kasing Apun

DOI:

https://doi.org/10.33736/bjrst.230.2015

Keywords:

Leptospira, national park, soil, Sarawak, water

Abstract

In Malaysia, there is an increasing number of reported leptospirosis cases which led to mortality. The infection is usually spread through the urine of infected animals and may contaminate the environmental soil and water. This study was conducted to determine the prevalence of Leptospira spp. in two national parks in Sarawak. A total of 50 soil and 60 water samples were collected from Tanjung Datu National Park (TDNP) and Bako National Park (BNP). The samples were filtered through sterile membrane filter prior to inoculation into modified semisolid Ellinghausen-McCullough-Johnson-Harris (EMJH) media added with 5-fluorouracil. The cultures were incubated at room temperature (28-30 °C) for 30 days before specific polymerase chain reaction (PCR) was conducted. PCR-positive samples were subjected to DNA sequencing. Out of the 110 environmental samples from two national parks in Sarawak, the prevalence of pathogenic, intermediate and saprophytic Leptospira was 0.9%, 5.5% and 0%, respectively. Results indicated that Leptospira borgpetersenii serovar Mini and Leptospira wolffii serovar Khorat were the pathogenic and intermediate Leptospira circulating in these study areas, respectively. Due to the ability of Leptospira to survive for months in environment, there is a risk of exposure to the public and tourists who visit these national parks. Increased awareness, continuous monitoring and effective preventive measures should be taken by local authorities to control leptospirosis outbreak.

References

Ahmed, A., Klaasen, H.L.B.M., van der Veen, M., van der Linden, H., Goris, M.G.A., & Hartskeerl, R.A. (2012). Evaluation of real-time PCR and culturing for the detection of Leptospires in canine samples. Advances in Microbiology, 2: 162-170.

https://doi.org/10.4236/aim.2012.22021

Backhaus, N. (2003). Non-place jungle: the construction of authenticity in national parks of Malaysia. Indonesia and the Malay World, 31(89): 151-160.

https://doi.org/10.1080/13639810304438

Benacer, D., Who, P.Y., Mohd Zain, S.N., Amran, F., & Thong, K.L. (2013). Pathogenic and saprophytic Leptospira species in water and soils from selected urban sites in Peninsular Malaysia. Microbes and Environment, 28(1): 138-140.

https://doi.org/10.1264/jsme2.ME12154

Cetinkaya, B., Ertas, H.B., Ongor, H., & Muz, A. (2000). Detection of Leptospira species by polymerase chain reaction (PCR) in urine of cattle. Turkish Journal of Veterinary and Animal Sciences, 24: 123-130.

Collares-Pereira, M., Korver, H., Cao Thi, B.V., Santos-Reis, M., Bellenger, E., Baranton, G., & Terpstra, W.J. (2000). Analysis of Leptospira isolates from mainland Portugal and the Azores islands. FEMS Microbiology Letters, 185: 181-187.

https://doi.org/10.1111/j.1574-6968.2000.tb09059.x

Desvars, A., Naze, F., Vourc'h, G., Cardinale, E., Picardeau, M., Michault, A., & Bourhy, P. (2012). Similarities in Leptospira serogroup and species distribution in animals and humans in the Indian Ocean Island of Mayotte. The American Journal of Tropical Medicine and Hygiene, 87(1): 134-140.

https://doi.org/10.4269/ajtmh.2012.12-0102

Diesch, S., McCulloch, W.F., Braun, J.L., & Crawford Jr, R.P. (1969). Environmental studies on the survival of Leptospires in a farm creek following a human leptospirosis outbreak in Iowa. Bulletin of the Wildlife Disease Association, 5: 166-173.

https://doi.org/10.7589/0090-3558-5.3.166

Evangelista, K.V. & Coburn, J. (2010). Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses. Future Microbiology, 5(9): 1413-1425.

https://doi.org/10.2217/fmb.10.102

Fentahun, T. & Alemayehu, M. (2012). Leptospirosis and its public health significance: A review. European Journal of Applied Sciences, 4(6): 238-244.

Guidugli, F., Castro, A.A., & Atallah, A.N. (2000). Systematic reviews on leptospirosis. Revista do Instituto de Medicina Tropical de Sao Paulo, 42(1): 47-49.

https://doi.org/10.1590/S0036-46652000000100008

Lim, J.K., Murugaiyah, V.A., Ramli, A., Abdul Rahman, H., Mohamed, N., Shamsudin, N., & Tan, J.C. (2011). A case study: Leptospirosis in Malaysia. Webmed Central Infectious Diseases, 2(12): WMC002764.

Mohan, A.R.M., Cumberbatch, A., Adesiyun, A.A., & Chadee, D.D. (2009). Epidemiology of human leptospirosis in Trinidad and Tobago, 1996-2007: A retrospective study. Acta Tropica, 112: 260-265.

https://doi.org/10.1016/j.actatropica.2009.08.007

Murgia, R., Riquelme, N., Baranton, G., & Cinco, M. (1997). Oligonucleotides specific for pathogenic and saprophytic Leptospira occurring in water. FEMS Microbiology Letters, 148(1): 27-34.

https://doi.org/10.1111/j.1574-6968.1997.tb10262.x

Ricaldi, J.N., Fouts, D.E., Selengut, J.D., Harkins, D.M., Patra, K.P., Moreno, A., Lehmann, J.S., Purushe, J., Sanka, R., Torres, M., Webster, N.J., Vinetz, J.M., & Matthias, M.A. (2012). Whole genome analysis of Leptospira licerasiae provides insight into leptospiral evolution and pathogenicity. PLOS Neglected Tropical Diseases, 6(10): e1853. doi:10.1371/journal.pntd.0001853.

https://doi.org/10.1371/journal.pntd.0001853

Ridzlan, F.R., Bahaman, A.R., Khairani-Bejo, S., & Mutalib, A.R. (2010). Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia. Tropical Biomedicine, 27(3): 632-638.

Sarawak Health Department. Communicable Disease Control Section (SHDCPRC). (2015). Sarawak Weekly Epid News, Week 9, 1-7 March 2015.

Sejvar, J., Bancroft, E., Winthrop, K., Bettinger, J., Bajani, M., Bragg, S., Shutt, K., Kaiser, R., Marano, N., Papovic, T., Tappero, J., Ashford, D., Mascola, L., Vugia, D., Perkins, B., Rosentein, N., & Eco-Challenge Investigation Team. (2003). Leptospirosis in "Eco-Challenge" Athletes, Malaysian Borneo. Emerging Infectious Diseases, 9: 702-707.

https://doi.org/10.3201/eid0906.020751

Sergio, A.D.M., Figueroa, E.G., Saadia, V.G.M., Elizabeth, S.H., Beatriz, R.S., Victor, M.A. A., & Espinosa, J.N. (2012). Leptospirosis prevalence in patients with initial diagnosis of dengue. Journal of Tropical Medicine. Volume 2012, Article ID 519701, 5 pages. doi:10.1155/2012/519701.

https://doi.org/10.1155/2012/519701

Slack, A.T., Kalambaheti, T., Symonds, M.L., Dohnt, M.F., Galloway, R.L., Steigerwalt, A.G., Chaicumpa, W., Bunyaraksyotin, G., Craig, S., Harrower, B.J., & Smythe, L.D. (2008). Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand. International Journal of Systematic and Evolutionary Microbiology, 58: 2305-2308.

https://doi.org/10.1099/ijs.0.64947-0

Slack, A.T., Symonds, M.L., Dohnt, M.F., & Smythe, L.D. (2006). Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiology, 6: 95.

https://doi.org/10.1186/1471-2180-6-95

Su'ut, L., Yusof, H., Arif, M.T., Rahim, N.A.A., Katip, J.T., & Suhaili, M.R. (2011). The prevalence of leptospirosis in the Rejang Basin of Sarawak. UNIMAS Research Update, 7(2): 5.

Tansuphasiri, U., Thipsuk, C., Phulsuksombati, D., & Chanyasanha, C. (2006). Duplex PCR-hybridization based detection of pathogenic Leptospira in environmental water samples obtained from endemic areas in northeast region of Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 37(4): 729-741.

Thayaparan, S., Robertson, I., Amran, F., Su'ut, L., & Abdullah, M.T. (2013). Serological prevalence of leptospiral infection in wildlife in Sarawak, Malaysia. Borneo Journal of Resource Science and Technology, 2(2): 79-82.

https://doi.org/10.33736/bjrst.281.2013

Uavechanichkul, R., Sraphet, S., Suwancharoen, D., & Triwitayakorn, K. (2011). PCR-based technique for detection and differentiation of pathogenic and saprophytic Leptospira species. International Journal of Microbiological Research, 2(1): 43-48.

Vein, J., Perrin, A., Berny, P.J., Benoit, E., Leblond, A., & Kodjo, A. (2012). Adaptation of a real-time PCR method for the detection and quantification of pathogenic leptospires in environmental water. Canadian Journal of Microbiology, 58: 828-835.

https://doi.org/10.1139/w2012-060

Victoriano, A.F.B., Smythe, L.D., Gloriani-Barzaga, N., Cavinta, L.L., Kasai, T., Limpakarnjanarat, K., Ong, B.L., Gongal, G., Hall, J., Coulombe, C.A., Yanagihara, Y., Yoshida, S-i., & Adler, B. (2009). Leptospirosis in the Asia Pacific Region. BMC Infectious Diseases, 9:147 doi:10.1186/1471-2334/9/147.

https://doi.org/10.1186/1471-2334-9-147

Villanueva, S.Y.A.M., Ezoe, H., Baterna, R.A., Yanagihara, Y., Muto, M., Koizumi, N., Fukui, T., Okamoto, Y., Masuzawa, T., Cavinta, L.L., Gloriani, N.G., & Yoshida, S-i. (2010). Serologic and molecular studies of Leptospira and Leptospirosis among rats in the Philippines. The American Journal of Tropical Medicine and Hygiene, 82(5): 889-898.

https://doi.org/10.4269/ajtmh.2010.09-0711

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How to Cite

Pui, C. F., Bilung, L. M., Su’ut, L., & Apun, K. (2016). Prevalence of Leptospira Species in Environmental Soil and Water from National Parks in Sarawak, Malaysia. Borneo Journal of Resource Science and Technology, 5(1), 49–57. https://doi.org/10.33736/bjrst.230.2015

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