Characterisation and Expression Analysis of Hydroxyphenylpyruvate Reductase derived from Orthosiphon aristatus

Authors

  • Zuliza Ahmad
  • Ahmad Husaini
  • Hairul Azman Roslan

DOI:

https://doi.org/10.33736/bjrst.226.2015

Keywords:

Phydroxyphenylpyruvate reductase (HPPR), Orthosiphon aristatus, rapid amplification of cDNA ends (RACE), rosmarinic acid

Abstract

Herbal products are getting more popular as alternative medicines and food supplements. The therapeutic effects of herbal medicines are mainly attributed to their bioactive secondary metabolites. Orthosiphon aristatus, locally known as ‘Misai Kucing’, is known for its various health benefits. One of the main chemical constituent of O. aristatus is rosmarinic acid, a plant polyphenol that has been proven to have antioxidant, anti-inflammatory and antimicrobial activities. Hydroxyphenylpyruvate reductase (HPPR) is one of the enzymes involved in rosmarinic acid biosynthetic pathway. Here we report the effect of UV on HPPR expression and the isolation of a full-length hppr cDNA from O. aristatus via rapid amplification of cDNA ends polymerase chain reaction. An increase in the expression was detected when the plant was exposed to UV and detected via the expression of hppr transcript. A 1116 bp nucleotide putative cDNA was isolated corresponding to 307 predicted amino acid. We have also isolated the 5’ and 3’ untranslated regions with a length of 54 bp and 123 bp, respectively. Sequence similarity analysis was performed against NCBI genebank and the BLAST result showed that the putative hppr cDNA isolated from O. aristatus exhibited high similarities with other hppr cDNA of the members of the Lamiaceae family such as Perilla frutescens, Salvia officinalis, Salvia miltiorrhiza and Solenostemon scutellarioides.

References

Abdelwahab, S.I., Mohan, S., Mohamed Elhassan, M., Al-Mekhlafi, N., Mariod, A.A., Abdul, A.B., Abdulla, M.A., & Alkharfy, K.M. (2011). Antiapoptotic and antioxidant properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervension in Bcl-2-mediated apoptotic pathway. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 156765.

https://doi.org/10.1155/2011/156765

Akowuah, G.A., Ismail, Z., Norhayati, I., & Sadikun, A. (2005). The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical-scavenging activity. Food Chemistry, 93: 311-317.

https://doi.org/10.1016/j.foodchem.2004.09.028

Barberini, S., Savona, M., Raffi, D., Leonardi, M., Pistelli, L., stocmal, A., Vainstein, A., Pistelli, L., & Ruffoni, B. (2013). Molecular cloning of SoHPPR encoding a hydroxyphenylpyruvate reductase, and its expression in cell suspension cultures of Salvia officinalis. Plant Cell, Tissue and Organ Culture, 114(1): 131-138.

https://doi.org/10.1007/s11240-013-0300-8

Chin, J.H., Hussin, A.H., & Ismail, S. (2009). Effect of Orthosiphon stamineus leaf extracts on hepatic cytochrome P450, UGT and GST activity in STZ-induced diabetic rats. Journal for the Advancement of Science and Arts, 1(1): 1-8.

Gasic, K., Hernandez, A., & Korban, S.S. (2004). RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Molecular Biology Reporter, 22: 437a-437g.

https://doi.org/10.1007/BF02772687

Hossain, M.A., Ismail, Z., Rahman, A., & Kang, S.C. (2008). Chemical composition and anti-fungal properties of the essential oils and crude extracts of Orthosiphon stamineus Benth. Industrial Crops and Products, 27: 328-334.

https://doi.org/10.1016/j.indcrop.2007.11.008

Iwai, M., Ohta, M., Tsuchiya, H., & Suzuki, T. (2010). Enhanced accumulation of caffeic acid and luteolin-glucoside in red perilla cultivated under red diode laser and blue LED illumination followed by UV-A irradiation. Journal of Functional Food, 2: 66-70.

https://doi.org/10.1016/j.jff.2009.11.002

Jaganath, I.B. & Ng, L.T. (2000). Herbs: The green pharmacy of Malaysia. Kuala Lumpur: Vinpress Sdn. Bhd. in collaboration with Malaysian Agricultural Research and Development Institute (MARDI).

Janiak, V., Petersen, M., Zentgraf, M., Klebe, G., & Heine, A. (2010). Structure and substrate docking of a hydroxyl(phenyl)pyruvate reductase from the higher plant Coleus blumei Benth. Acta Crystallographica, 66(5): 593-603.

https://doi.org/10.1107/S0907444910006360

Khamsah, S.M., Akowuah, G., & Zhari, I. (2006). Antioxidant activity and phenolic content of Orthosiphon stamineus Benth from different geographical origin. Journal of Sustainability Science and Management, 1(2): 14-20.

Kim, K.H., Janiak, V., & Petersen, M. (2004). Purification, cloning and functional expression of hydroxyphenylpyruvate reductase involved in rosmarinic acid biosynthesis in cell cultures of Coleus blumei. Plant Molecular Biology, 54: 311-323.

https://doi.org/10.1023/B:PLAN.0000036367.03056.b2

Kiong, A.L.P., Lai, A.G., Hussein, S., & Harun, A.R. (2008). Physiological responses of Orthosiphon stamineus to gamma radiation. American-Eurasian Journal of Sustainable Agriculture, 2(2): 135-149.

Li, W., Koike, K., Asada, Y., Yoshikawa, T., & Nikaido, T. (2005). Rosmarinic acid production by Coleus forskohlii hairy root cultures. Plant Cell, Tissue and Organ Culture, 80: 151-155.

https://doi.org/10.1007/s11240-004-9541-x

Luis, J.C., Pérez, R.M., & González, F.V. (2007). UV-B radiation effects on foliar concentrations of rosmarinic and carnosic acids in rosemary plants. Food Chemistry, 101: 1211-1215.

https://doi.org/10.1016/j.foodchem.2006.03.023

Matsuura, H.N., Costa, F., Yendo, A.C.A., & Fett-Neto, G. (2013). Photoelicitation of bioactive secondary metabolites by ultraviolet radiation: mechanisms, strategies and applications. In Chandra, S., Lata, H. & Varma, A. (eds), Biotechnology for Medicinal Plants, Springer-Verlag Berlin Heidelberg. Pp 171-190.

https://doi.org/10.1007/978-3-642-29974-2_7

Nicot, N., Hausman, J. Hoffmann, L., & Evers, D. (2005). Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. Journal of Experimental Botany, 56(421): 2907-2914.

https://doi.org/10.1093/jxb/eri285

Paul, N.D. & Gwynn-Jones, D. (2003). Ecological roles of solar UV radiation: towards an integrated approach. Trends in Ecology and Evolution, 18(1): 48-55.

https://doi.org/10.1016/S0169-5347(02)00014-9

Petersen, M. & Simmonds, M.S.J. (2003). Rosmarinic acid. Phytochemistry, 62: 121-125.

https://doi.org/10.1016/S0031-9422(02)00513-7

Petersen, M., Abdullah, Y., Benner, J., Eberle, D., Gehlen, K., Hucherig, S., Janiak, V., Kim, K.H., Sander, M., Weitzel, C., & Wolters, S. (2009). Evolution of rosmarinic acid biosynthesis. Phytochemistry, 70: 1663-1679.

https://doi.org/10.1016/j.phytochem.2009.05.010

Salleh, S.A., Rajab, N.F., Abdullah, N.R., Ismail, Z., Mouatt, P., Dowell, A., & Muhamad, S. (2011). In vitro chemopreventive activity of an ethyl acetate fraction derived from hot water extract of Orthosiphon stamineus in HepG2 cells. Journal of Medicinal Plants Research, 5(10): 1892-1899.

Tezuka, Y., Stampoulis, P., Banskota, A.H., Awale, S., Tran, K.Q., Saiki, I., & Kadota, S. (2000). Constituents of the Vietnamese medicinal plant Orthosiphon stamineus. Chemical and Pharmaceutical Bulletin, 48(11): 1711-1719.

https://doi.org/10.1248/cpb.48.1711

Tong, W.Y., Darah, I., & Latiffah, Z. (2011). Antimicrobial activities of endophytic fungal isolates from medicinal herb Orthosiphon stamineus Benth. Journal of Medicinal Plants Research, 5(5): 831-836.

Yam, M.F., Ang, L.F., Basir, R., Salman, I.M., Ameer, O.Z., & Asmawi, M.Z. (2009). Evaluation of the anti-pyretic potential of Orthosiphon stamineus Benth standardized extract. Inflammopharmacology, 17: 50-54.

https://doi.org/10.1007/s10787-008-8038-3

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How to Cite

Ahmad, Z., Husaini, A., & Roslan, H. A. (2016). Characterisation and Expression Analysis of Hydroxyphenylpyruvate Reductase derived from Orthosiphon aristatus. Borneo Journal of Resource Science and Technology, 5(1), 34–42. https://doi.org/10.33736/bjrst.226.2015

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