Application of Real Time PCR for the Detection and Enumeration of Vibrio parahaemolyticus from Seafood in Sarawak (Malaysia)
Abstract
Vibrio parahaemolyticus has been associated as the major cause of food poisoning and gastroenteritis in manycountries including Southeast Asia. Their human infection is regularly associated with the consumption of raw
or undercooked seafood and contaminated water supplies. A rapid method of detection is important to monitor
the occurrence of V. parahaemolyticus in both food and environment samples especially in Malaysia. Thus, the
aim of this study was to detect and enumerate V. parahaemolyticus from seafood by using the real-time PCR
based on the SYBR green assay. targeting the thermolabile (tl) gene. The assay was applied on 24 seafood
samples comprised of 6 cockles, 6 prawns, 6 squids and 6 fishes. In this study, all of the six cockles and prawns
were positive for the presence of V. parahaemolyticus while fish samples have only 2 positive isolates.
However, this study recorded no presence of V. parahaemolyticus in squids. Overall, the real-time PCR assay
was proven to be highly specific, and is sensitive in detecting and enumerating V. parahaemolyticus in the
seafood samples. In addition, this study has proven that seafood (especially cockles and prawns) are potential
sources for V. parahaemolyticus.
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