Heavy Metal Tolerance of Klebsiella pneumoniae Kpn555 Isolated from Coffee Pulp Waste



Microorganisms gain the ability to tolerate complex pollutants such as heavy metals, pesticides and polyaromatic hydrocarbons on continual exposure to them. The ability of indigenous microorganisms in tolerance and degradation of persistent xenobiotics has been frequently exploited. Coffee pulp waste is known to contain trace amounts of heavy metals attributing to residues from agro industrial activities. In the present study, a Gram negative non motile rod shaped bacterium, isolated from coffee pulp waste, showed tolerance to heavy metals such as cadmium (Cd), lithium (Li) and mercury (Hg). It was identified as Klebsiella pneumoniae Kpn555 by 16s RNA sequencing. The bacterium showed a minimum inhibitory concentration of 150 mg/L, 250 mg/L and 10 mg/L of Cd, Li and Hg, respectively. When the bacterium was grown in nutrient broth supplemented with concentrations of Hg (5 and 10 mg/L), Cd (50 and 100 mg/L) and Li (100 and 200 mg/L), it was observed that the growth reduced with the increase in concentration of heavy metals. The residual heavy metal concentration in the cell free supernatant was determined and the percentage of reduction in the concentration of heavy metals was calculated. It was observed that the cell free broth had a reduction of 54.8%, 50.6% and 40.6% of Li, Cd and Hg, respectively. This means that the bacterium has adsorbed the heavy metals from the medium onto their cell wall or inside the cytoplasm. This study revealed that K. pneumoniae Kpn555 has multi heavy metal resistance which could be utilised for bioremediation of soil and water polluted with multiple heavy metals.


Abbas, S.Z., Rafatullah, M., Ismail, N. & Lalung, J. (2014). Isolation, identification, and characterization of cadmium resistant Pseudomonas sp. M3 from industrial wastewater. Journal of Waste Management, 2014: 1-6.


Balan, B.M., Shini, S., Krishnan, K.P. & Mohan, M. (2018). Mercury tolerance and biosorption in bacteria isolated from Ny-Ă…lesund, Svalbard, Arctic. Journal of Basic Microbiology, 58(4): 286-295. https://doi.org/10.1002/jobm.201700496


Beyersmann, D. & Hartwig, A. (2008). Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms. Archives of Toxicology, 82(8): 493-512. https://doi.org/10.1007/s00204-008-0313-y.


Dadi, D., Daba, G., Beyene, A., Luis, P. & Van der Bruggen, B. (2019). Composting and co-composting of coffee husk and pulp with source-separated municipal solid waste: a breakthrough in valorization of coffee waste. International Journal of Recycling of Organic Waste in Agriculture, 8(3): 263-277. https://doi.org/10.1007/s40093-019-0256-8.


David, M., Krishna, P.M. & Sangeetha, J. (2016). Elucidation of impact of heavy metal pollution on soil bacterial growth and extracellular polymeric substances flexibility. 3 Biotech, 6(2): 172. doi:10.1007/s13205-016-0475-x


Degefe, G., Mengistou, S. & Mohammed, S. (2016). Physico chemical evaluation of coffee husk, wastes of enset (Enset ventricosum), vegetable and khat (Catha edulis) through vermicomposting employing an epigeic earthworm Dendrobaena veneta (Rosa, 1886). African Journal of Biotechnology, 15(20): 884-890 https ://doi.org/10.5897/AJB20 15.14676.


Ezaka, E. & Anyanwa, C.U. (2011). Chromium (VI) tolerance of bacterial strains isolated from sewage oxidation ditch. International Journal of Environmental Sciences, 1(7): 1725-1734.

Goyal, B., Sharma, R. & Mehta, P. (2015). Treatment of toxic pollutants from waste water using novel technique. Current Research in Pharmaceutical Sciences, 05(01):1-11.

Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B.B., Beeregowda, K.N. (2014). Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology, 7(2): 60-72. doi:10.2478/intox-2014-0009.


Kafilzadeh, F., Moghtaderi, Y. & Jahromi, A.R. (2013). Isolation and identification of cadmium-resistant bacteria in Soltan Abad river sediments and determination of tolerance of bacteria through MIC and MBC. European Journal of Experimental Biology, 3(5): 268-273.

Khan, Z., Hussain, S.Z., Rehman, A., Zulfiqar, S. & Shakoori, A.R. (2015). Evaluation of cadmium resistant bacterium, Klebsiella pneumoniae, isolated from industrial wastewater for its potential use to bioremediate environmental cadmium. Pakistan Journal of Zoology, 47(6): 1503-1513.


Koptsik, G.N. (2014). Problems and prospects concerning the phytoremediation of heavy metal polluted soils: A review. Eurasian Soil Science, 47(9): 929-939.


Li, Z., Ma, Z., van der Kuijp, T. J., Yuan, Z., Huang, L. (2014). A review of soil heavy metal pollution from mines in China: Pollution and health risk assessment. Science of the Total Environment, 468: 843-853.


Marzan, L.W., Hossain, M., Mina, S.A., Akter, Y. & Chowdhury, A.M.M.A. (2017). Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint. Egyptian Journal of Aquatic Research, 43(1): 65-74.


Zafra, G., Regino, R., Agualimpia, B. & Aguilar, F. (2016). Molecular characterization and evaluation of oil-degrading native bacteria isolated from automotive service station oil-contaminated soils. Chemical Engineering Transactions, 49: 511-516.

How to Cite
APARNA K MOHAN, SHINY MARTIS B, SANJANA CHIPLUNKAR, SANDHYA KAMATH, LOUELLA CONCEPTA GOVEAS, & C VAMAN RAO. (2019). Heavy Metal Tolerance of Klebsiella pneumoniae Kpn555 Isolated from Coffee Pulp Waste. Borneo Journal of Resource Science and Technology, 9(2), 101-106. Retrieved from https://publisher.unimas.my/ojs/index.php/BJRST/article/view/2015