Physicochemical and Antioxidant Properties of Copper-Amaranth Leaf Purees Using Viscozyme-L Enzymatic Liquefaction
Keywords:
Amaranth, cell wall-degrading enzyme, chlorophyll content, Viscozyme-LAbstract
This study evaluated the effect of cell wall-degrading enzyme (Viscozyme® L) on the physicochemical properties and antioxidant activity of copper-amaranth (Amaranthus Viridis Linn.) leaf purees. The purees were liquefied with varying concentrations of Viscozyme® L (0–3% v/w) over different incubation times (30 minutes to 24 hours). It was observed that treatment with 1% Viscozyme® L (v/w) at pH 5 for 3 hours, followed by incubation at 45 °C, resulted in a significant increase in total soluble solids (°Brix), acidity, and total chlorophyll content. The enzyme-treated purees exhibited higher DPPH (13.52 mM (TE)/g fresh weight), and FRAP (6.04 mM (TE)/g fresh weight) values, as well as in reducing sugar (118.43 mg/mL), soluble dietary fibre (4.32%) content and greener colour (-0.35) values, as compared to non-enzyme treated purees (0.13 mg/g, 9.47 mM (TE)/g and 3.72 mM (TE)/g fresh weight, and 12.75 mg/mL, 0.60%, and -0.28, respectively). These findings demonstrate that treatment with Viscozyme® L enzymes can effectively improve the nutritional and functional quality of vegetable-based purees, with potential applications in the processing of green vegetable juices.
References
Akbar, S., Rahman, R., Mushtaq, A., Azeem, M.W., & Al Mahruqi, Z.M. (2017). Slender Amaranth: A review on botany, chemistry, pharmacological importance and potential benefits. International Journal of Chemical and Biochemical Sciences, 12: 152-156.
Amobonye, A. E., Bhagwat, P., Ruzengwe, F. M., Singh, S. & Pillai, S. (2022). Pear juice clarification using polygalacturonase from Beauveria bassiana: Effects on rheological, antioxidant and quality properties. Polish Journal of Food and Nutrition Sciences, 72(1): 57-67. https://doi.org/10.31883/pjfns/145704
Amin, I., Norazaidah, Y., & Hainida, K.I.E. (2006). Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chemistry, 94(1): 47-52. https://doi.org/10.1016/j.foodchem.2004.10.048
Arsad, P., Wan Ibadullah, W.Z Mustapha, W.Z., Meor Hussin, A.S. & Sukor, R. (2015). Effects of enzymatic treatment on physicochemical properties of sugar palm fruit juice. International Journal of Advanced Science Engineering Information Technology, 5 (5): 308–312. https://doi.org/10.18517/ijaseit.5.5.577
Bashir, O., Hussain, S.Z. & Gani, G. (2021). Evaluating the physicochemical and antioxidant characteristics of apricot juice prepared through pectinase enzyme-assisted extraction from Halman variety. Food Measure. 15, 2645-2658. https://doi.org/10.1007/s11694-021-00833-w
Benzie, I.F. & Strain, J. (1996). The ferric-reducing ability of plasma (FRAP) is a measure of "antioxidant power". The FRAP assay. Analytical biochemistry, 239 (1): 70-76. https://doi.org/10.1006/abio.1996.0292
Brand-Williams, W., Cuvelier, M. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1): 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Buckenhüskes, H., Omran, H., Zhang, C. & Gierschner, K. (1990). Investigations on enzymatic liquefaction of red and white cabbage. Food Biotechnology, 4(1): 289-299. https://doi.org/10.1080/08905439009549741
Chauhan, M., Thakur, N.S., Thakur, A. & Hamid. (2017). Standardisation of enzymatic treatments for the extraction of juice from wild prickly pear (Opuntia dillenii Haw.). Indian Journal of Ecology, 44(6): 715-720.
Chawla, R. & Patil, G.R. (2010). Soluble dietary fibre. Comprehensive Reviews in Food Science and Food Safety, 9(2): 178-196. https://doi.org/10.1111/j.1541-4337.2009.00099.x
Chong, S.Y., & Wong, C.W. (2015). Production of spray-dried sapodilla (Manilkara zapota) powder from enzyme-aided liquefied puree. Journal of Food Processing and Preservation, 39: 2604-2611. https://doi.org/10.1111/jfpp.12510
Costache, Manuela, A., Gheorghe, C. & Gabriela, N. (2012). Studies concerning the extraction of chlorophyll and total carotenoids from vegetables. Romanian Biotechnological Letters, 17(5): 7703-7708.
Dere, S., Günes, T. & Sivaci, R. (1998). Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany, 22: 13-17.
Dias, J.S. & Ryder, E. (2011). World vegetable industry. Production, Breeding, Trends. Hort Review, 38: 299-356. https://doi.org/10.1002/9780470872376.ch8
Dueñas, M., Hernández, T. & Estrella, I. (2007). Changes in the content of bioactive polyphenolic compounds of lentils by the action of exogenous enzymes. Effect on their antioxidant activity. Food Chemistry, 101(1): 90-97. https://doi.org/10.1016/j.foodchem.2005.11.053
Galante, Y.M., De Conti, A. & Monteverdi, R. (1998). Application of Trichoderma enzymes in food and feed industries. In Harman, G.F. & Kubicek, C.P. (Eds), Trichoderma and Gliocladium-Enzyme, Biological Control and Commercial Applications. London, Taylor and Francis. pp. 327-342.
Godfrey, T. & West, S. (1996). Introduction to industrial enzymology. London. Macmillan Press. pp. 1-8.
Grabowski, J.A., Truong, V.D. & Daubert, C.R. (2008). Nutritional and rheological characterisation of spray-dried sweet potato powder. LWT - Food Science and Technology, 41(2): 206-216. https://doi.org/10.1016/j.lwt.2007.02.019
Grabowski, J., Truong, V.D. & Daubert, C. (2006). Spray‐Drying of amylase hydrolysed sweet potato puree and physicochemical properties of the powder. Journal of Food Science, 71(5): E209-E217. https://doi.org/10.1111/j.1750-3841.2006.00036.x
Gurrieri, S., Miceli, L., Lanza, M., Tomaselli, F., Bonomo, R. P. & Rizzarelli, E. (2000). Chemical characterisation of Sicilian prickly pear (Opuntia ficus indica) and perspective for the storage of its juice. Journal of Agriculture and Food Chemistry, 48: 5424- 5431. https://doi.org/10.1021/jf9907844
Guzmán, G. R., Dorantes, A. L., Hernández, U. H., Hernández, S. H., Ortiz, A. & Mora, E. R. (2002). Effect of zinc and copper chloride on the color of avocado puree heated with microwaves. Innovative Food Science and Emerging Technologies, 3(1): 47-53. https://doi.org/10.1016/S1466-8564(01)00053-4
Hesham, A. E. & Manal, F. S. (2015). Effect of incubation, enzymes and thermal pre-treatments on the quality of pumpkin juice. Journal of Nutrition and Food Science, 5: 371. https://doi.org/10.4172/2155-9600.1000371
Hong, Y. H., Jung, E. Y., Park, Y., Shin, K. S., Kim, T. Y., Yu, K. W., Chang, U. J. & Suh, H. J. (2013). Enzymatic improvement in the polyphenol extractability and antioxidant activity of green tea extracts. Bioscience, Biotechnology, and Biochemistry, 77(1): 22-29. https://doi.org/10.1271/bbb.120373
Humphrey A.M. (2004). Chlorophyll as a color and functional ingredient. J. Food Sci., 69: C422–C425. https://doi.org/10.1111/j.1365-2621.2004.tb10710.x
Isabelle, M., Lee, B. L., Lim, M. T., Koh, W. P., Huang, D. & Ong, C. N. (2010). Antioxidant activity and profiles of common fruits in Singapore. Food Chemistry, 123: 77–84. https://doi.org/10.1016/j.foodchem.2010.04.002
Jiménez-Aguilar, D.M. & Grusak, M.A. (2015). Evaluation of minerals, phytochemical compounds and antioxidant activity of Mexican, Central American and African green leafy vegetables. Plant Foods for Human Nutrition, 70: 357–364. https://doi.org/10.1007/s11130-015-0512-7
Kashyap, D.R., Vohra, P.K., Chopra, S. & Tewari, R. (2001). Applications of pectinases in the commercial sector. A review. Bioresource Technology, 77: 215- 227. https://doi.org/10.1016/s0960-8524(00)00118-8
Koley, T. K., Walia, S., Nath, P., Awasthi, O. & Kaur, C. (2011). Nutraceutical composition of Zizyphus mauritiana Lamk (Indian ber): Effect of enzyme-assisted processing. International Journal of Food Sciences and Nutrition, 62(3): 276-279. https://doi.org/10.3109/09637486.2010.526930
Kotcharian, A., Kunzek, H. & Dongowski, G. (2004). The influence of variety on the enzymatic degradation of carrots and on functional and physiological properties of the cell wall materials. Food Chemistry,87(2): 231-245. https://doi.org/10.1016/j.foodchem.2003.11.015
Lee, S. C., Prosky, L. & De Vries, J. (1992). Determination of total, soluble, and insoluble dietary fibre in foods. enzymatic-gravimetric method, MES-TRIS buffer. collaborative study. Journal of AOAC International (USA), 75: 395-416.
Lee, W.C., Yusof, S., Hamid, N.S.A. & Baharin, B.S. (2006). Optimising conditions for enzymatic clarification of banana juice using response surface methodology (RSM). Journal of Food Engineering, 73(1): 55-63. https://doi.org/10.1016/j.jfoodeng.2005.01.005
Leunda, Maria, A., Norma, G. & Stella, M.A. (2000). Color and chlorophyll content changes of minimally processed kiwifruit. Journal of Food Processing and Preservation, 24(1): 17-38. https://doi.org/10.1111/j.1745-4549.2000.tb00403.x
Li, B.B., Smith, B. & Hossain, M.M. (2006). Extraction of phenolics from citrus peels. II. Enzyme-assisted extraction method. Separation and Purification Technology, 48(2): 189-196. https://doi.org/10.1016/j.seppur.2005.07.019
Li, H.B., Wong, C.C., Cheng, K.W. & Chen, F. (2008). Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Science and Technology, 41(3): 385-390. https://doi.org/10.1016/j.lwt.2007.03.011
Liew Abdullah, A. G., Sulaiman, N. M., Aroua, M. K. & Megat Mohd Noor, M. J. (2007). Response surface optimisation of conditions for clarification of carambola fruit juice using a commercial enzyme. Journal of Food Engineering, 81(1): 65-71. https://doi.org/10.1016/j.jfoodeng.2006.10.013
Mavhungu, N. (2011). Antioxidant properties and cellular protective effects of selected african green leafy vegetables. PhD Thesis. Retrieved from http:// repository.up.ac.za/handle/2263/25198. University of Pretoria, South Africa.
Norjana, I. & Noor Aziah, A.A. (2012). Quality attributes of Durian (Durio zibethinus Murr) juice after pectinase enzyme treatment. International Food Research Journal, 18: 1117-1122.
Östbring, Karolina, Marilyn, R., Ingegerd, S., Jennie, O. & Charlotte, E.A. (2014). The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/co-lipase activity. Food and Function, 5 (9): 2157-2165. https://doi.org/10.1039/c3fo60651a
Özkan, G. & Bilek, S. E. (2015). Enzyme-assisted extraction of stabilised chlorophyll from spinach. Food Chemistry, 176: 152-157. https://doi.org/10.1016/j.foodchem.2014.12.059
Pocock, T., Król, M. & Huner, N. P. (2004). The determination and quantification of photosynthetic pigments by reverse phase high-performance liquid chromatography, thin-layer chromatography, and spectrophotometry. Photosynthesis Research Protocols: Springer: 137-148. https://doi.org/10.1385/1-59259-799-8:137
Puri, M., Sharma, D. & Barrow, C. J. (2012). Enzyme-assisted extraction of bioactives from plants. Trends in Biotechnology, 30(1): 37-44. https://doi.org/10.1016/j.tibtech.2011.06.014
Roslan, J., Ling, H.C., Sintang, M.D. & Saallah, S. (2020). Effect of heat treatment on rheological properties of bambangan (Mangifera pajang kosterm) fruit juice. Advances in Agricultural and Food Research Journal, 1(2). https://doi.org/10.36877/aafrj.a0000115
Sakhale, B., Pawar, V. & Gaikwad, S. (2016). Studies on effect of enzymatic liquefaction on quality characteristics of Kesar mango pulp. International Food Research Journal, 23(2): 860-865.
Saqib, A.A.N. & Whitney, P.J. (2011). Differential behaviour of the dinitrosalicylic acid (DNS) reagent towards mono- and di-saccharide sugars. Biomass and Bioenergy, 35(11): 4748-4750. https://doi.org/10.1016/j.biombioe.2011.09.013
Schweiggert, U., Hofmann, S., Reichel, M., Schieber, A. & Carle, R. (2008). Enzyme-assisted liquefaction of ginger rhizomes (Zingiber officinale Rosc.) for the production of spray-dried and paste-like ginger condiments. Journal of Food Engineering, 84(1): 28-38. https://doi.org/10.1016/j.jfoodeng.2007.04.013
Sharma, A., Sarkar, B. & Sharma, H. (2005). Optimisation of enzymatic process parameters for increased juice yield from carrot (Daucus carota L.) using response surface methodology. European Food Research and Technology, 221(1-2): 106-112. http://dx.doi.org/10.1007/s00217-005-1203-7
Shin, K. S. & Lee, J. H. (2021). Optimisation of enzymatic hydrolysis of immature citrus (Citrus unshiu Marcov.) for flavonoid content and antioxidant activity using a response surface methodology. Food science and biotechnology, 30(5): 663–673. https://doi.org/10.1007/s10068-021-00897-w
Siti Faridah M.A., Yusof, Y.A., Karim, R. & Muhammad, K. (2021). Effects of Enzymatic Liquefaction, Drying Techniques, and Wall Materials on the Physicochemical Properties, Bioactivities, and Morphologies of Zinc-Amaranth (Amaranthus viridis L.) Powders. International Journal of Food Science, 2021: 1819104. https://doi.org/10.1155/2021/1819104
Stoll, T., Schweiggert, U., Schieber, A. & Carle, R. (2003). Process for the recovery of a carotene-rich functional food ingredient from carrot pomace by enzymatic liquefaction. Innovative Food Science and Emerging Technologies, 4(4): 415-423. http://dx.doi.org/10.1016/S1466-8564(03)00060-2
Sun, T., Powers, J. R. & Tang, J. (2007). Effect of enzymatic macerate treatment on rutin content, antioxidant activity, yield, and physical properties of asparagus juice. Journal of Food Science, 72(4): S267-S271. https://doi.org/10.1111/j.1750-3841.2007.00345.x
Tochi, B.N., Wang, Z., Xu, S.Y. & Zhang, W.B. (2009). The influence of a pectinase and pectinase/hemicellulases enzyme preparations on percentage pineapple juice recovery, particulates and sensory attributes. Pakistan Journal of Nutrition, 8(8): 1184-1189. http://dx.doi.org/10.3923/pjn.2009.1184.1189
Uhlig, H. (1998). Industrial enzymes and their applications, New York. John Wiley and Sons, Inc. pp. 435.
Umsza-Guez, M. A., Rinaldi, R., Lago-Vanzela, E. S., Martin, N., Silva, R. & Thoméo, J. C. (2011). Effect of pectinolitic enzymes on the physical properties of caja-manga (Spondias cytherea Sonn.) pulp. Food Science and Technology (Campinas), 31(2): 517-526. https://doi.org/10.1590/S0101-20612011000200037
Urlaub, R. (2002). Modern use of enzymes in fruit processing. Fruit Processing, 8: 360-361.
Wargovich, M.J. (2000). Anticancer properties of fruits and vegetables. Hort Science, 35: 573-575. https://doi.org/10.21273/HORTSCI.35.4.573
Weinberg, Z., Szakacs, G., Linden, J. & Tengerdy, R. (1990). Recovery of protein and chlorophyll from alfalfa by simultaneous lactic acid fermentation and enzyme hydrolysis (ENLAC). Enzyme and Microbial Technology, 12(12): 921-925.
Wong, C., Pui, L. & Ng, J. (2015). Production of spray-dried Sarawak pineapple (Ananas comosus) powder from enzyme liquefied puree. International Food Research Journal, 22(4).
Wrolstad, R.E., Acree, T.E., Decker, E.A., Penner, M., Reid, D., Schwartz, S., Shoemaker, C., Smith, D. & Sporns, P. (2005). Pigments, Colorants, Flavors, Texture, and Bioactive Food Components. In Handbook of Food Analytical Chemistry, John Wiley and Sons, Inc.
Yusof, S. & Ibrahim, N. (1994). Quality of soursop juice after pectinase enzyme treatment. Food Chemistry, 51(1): 83-88. https://doi.org/10.1016/0308-8146(94)90052-3
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