Polyherbal Formulation of Apium graveolens, Tithonia diversifolia and Curcuma longa acts as an Antidiabetic Agent Through the Regulation of Nrf2 Modulating Biochemical Parameters
Keywords:
antidiabetic, Apium graveolens, Curcuma longa, diabetes mellitus, Tithonia diversifoliaAbstract
Nuclear factor erythroid 2-related factor 2 (Nrf2), superoxide dismutase (SOD), malondialdehyde (MDA) and biochemical clinic are molecular components associated with metabolic dysfunctions in diabetes mellitus (DM). Alternative therapeutic approaches for diabetes mellitus may include the use of bioactive compounds derived from natural sources. This study aimed to evaluate the effect of a formulation of celery (Apium graveolens), paitan (Tithonia diversifolia) and turmeric (Curcuma longa), known as SPK, for diabetes treatment. Diabetes was induced in mice through multiple intraperitoneal doses of streptozotocin (50 mg/kg BW for 4 days). The diabetic mice were divided into five groups: healthy mice (N), DM (diabetic mice without treatment), diabetic mice treated with SPK extracts (SPK 1, SPK 2 and SPK 3). The treatments were administered orally once daily for 21 days. On day 22, all mice were euthanised and organ (liver) and blood samples were collected. The liver was analysed using flow cytometry to determine the profiles of Nrf2, SOD and MDA. Blood samples were analysed for biochemical parameter concentrations. All data were statistically analysed using one-way ANOVA (p≤0.05) with SPSS, followed by the Tukey test. The results showed that SPK administration significantly increased Nrf2 and SOD profiles under diabetic conditions but did not significantly affect the MDA profile. Additionally, in biochemical parameters, SPK administration significantly reduced triglyceride, blood glucose, creatinine, urea/BUN, SGOT and SGPT levels. In conclusion, the SPK formulation has the potential to be used as an alternative treatment for diabetes mellitus.
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