Enhancing Germination Efficiency in Dalbergia latifolia Roxb. (Fabaceae) through Seed Pre-treatment
DOI:
https://doi.org/10.33736/bjrst.10072.2026Keywords:
Dalbergia latifolia, seed germination, gibberellic acid, seed treatmentAbstract
Dalbergia latifolia, commonly known as Indian rosewood, is found in the Terai and distributed up to 1000 m in the Chure region of Nepal. This slow-growing species is threatened by overexploitation and illegal logging for its timber. This research was carried out to examine different seed treatment methods for the germination of D. latifolia. The primary objective of our research was to investigate the effect of various treatments and determine the most effective seed treatment method for enhancing seed germination of this species. The fallen pods from mother trees at Thulo Garduwa, Sindhuli, were collected in March. From the sun-dried fruits, the seeds were manually extracted and treated with water, 300 ppm Gibberlic Acid (GA₃), Sodium Hydroxide (NaOH) and Potassium Hydroxide (KOH). The treated and untreated (control) seeds were sown in completely randomized designed (CRD) plots inside the greenhouse. The germination data was recorded and analyzed. The germination parameters, like germination percentage (GP), total length of seedlings, vigor index (VI), germination rate (GR) and mean germination time (MGT), were calculated from the collected data. The result indicated that the seed treated with 300 ppm GA₃ showed the best germination with higher GP (53.67), total length (18.16 cm), VI (976.46), GR (6.94) and shorter MGT (8.01 days). Similarly, the least germination was given by KOH with GP (31.33) and GR (3.11), however, minimum seedling length (12.74 cm) and VI (557.62) were observed for the control. Surprisingly, KOH showed the best performance after GA₃ for seedling length. In conclusion, D. latifolia seeds possess low to moderate physiological dormancy and treatment with 300 ppm GA₃ significantly improved both germination performance and seedling growth.
References
Abdul-Baki, A.A. & Anderson, J.D. (1973). Vigor determination in soyabean seed by multiple criteria. Crop Science, 13: 630–633. DOI: 10.2135/cropsci1973.0011183X001300060013x.
Al-Absi, K.M. (2010). The effects of different pre-sowing seed treatments on breaking the dormancy of mahaleb cherries, Prunus mahaleb L. seeds. Seed Science and Technology, 38 (2): 332–340. DOI: 10.15258/sst.2010.38.2.06.
Arunkumar, A.N., Warrier, R.R., Kher, M.M. & Teixeira da Silva, J.A. (2022). Indian rosewood (Dalbergia latifolia Roxb.): biology, utilisation and conservation practices. Trees, 36 (3): 883–898. DOI: 10.1007/s00468021-02243-3.
Awasthi, P., Karki, H., Vibhuti, Bargali, K. & Bargali, S.S. (2016). Germination and seedling growth of pulse crop (Vigna Spp.) as affected by soil salt stress. Current Agriculture Research, 4 (2). DOI: 10.12944/CARJ.4.2.05.
Baskin, J.M. & Baskin, C.C. (2004). A classification system for seed dormancy. Seed Science Research, 14 (1): 1–16. DOI: 10.1079/SSR2003150.
Damaiyani, J. & Prabowo, H. (2019). Conservation strategy of a vulnerable species of ‘Rosewood’ (Dalbergia latifolia Roxb.) by insect pollinator identification. Journal of Physics: Conference Series, 1363 (1). IOP Publishing. pp. 012005. DOI: 10.1088/1742-6596/1363/1/012005.
Datt, G., Chauhan, J.S. & Ballabha, R. (2017). Influence of pre-sowing treatments on seed germination of various accessions of Timoor (Zanthoxylum armatum DC.) in the Garhwal Himalaya. Journal of Applied Research on Medicinal and Aromatic Plants, 7: 89–94. DOI: 10.1016/j.jarmap.2017.06.004.
Ellis, R.H. & Roberts, E.H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 373–409.
Gaikwad, R.S. & Borkar, S.U. (2017). Effect of pre-sowing treatments on seed germination and plant growth of syzygium cumuni (L.) Skeels. International Journal of Research and Analytical Reviews, 5 (4): 186–189.
Gaisamudre Kavita, N. & Dhabe Arvind S. (2011). Propagation of Meizotropis buteiformis Voigt. Journal of Ecobiotechnology, 3 (6): 13–15.
Ghosh, P.K. & Maiti, T.K. (2014). Effect of seed scarification on in vitro seed germination of Abrus precatorius L. Plant Archive, 14 (2): 881–885.
Groves, M. & Rutherford C. (2016). CITES and Timber. A guide to CITES-listed tree species.
Harsh, N.S.K., Dadwal, V.S. & Jamaluddin, J. (1992). A new post-emergence damping off disease of Eucalyptus seedlings. Indian Forester, 118 (4): 279–283. DOI: 10.36808/if/1992/v118i4/8423.
Hu, X.W., Wu, Y.P., Ding, X.Y., Zhang, R., Wang, Y.R., Baskin, J.M., & Baskin, C.C. (2014). Seed dormancy, seedling establishment and dynamics of the soil seed bank of Stipa bungeana (Poaceae) on the Loess Plateau of northwestern China. PLoS One, 9 (11): e112579. DOI: 10.1371/journal.pone.0112579.
Karam, N.S. & Al-Salem, M.M. (2001). Breaking dormancy in Arbutus andrachne L. seeds by stratification and gibberellic acid. Seed Science and Technology, 29 (1): 51–56.
Kazembe, T., Munyarari, E. & Charumbira, I. (2012). Use of traditional herbal medicines to cure malaria. Bulletin of Environment, Pharmacology and Life Sciences, 1 (4): 63–85.
Khare, C.P. (2008). Indian medicinal plants: an illustrated dictionary. Springer Science & Business Media.
Koutouan-Kontchoi, M.N., Phartyal, S.S., Rosbakh, S., Kouassi, E.K. & Poschlod, P. (2020). Seed dormancy and dormancy breaking conditions of 12 West African woody species with high reforestation potential in the forest-savanna ecotone of Côte d'Ivoire. Seed Science Technology, 48 (1): 101–116. DOI: 10.15258/sst.2020.48.1.12.
Kumar, U. & Chavan, R.L. (2018). Effect of pre-sowing treatments on germination parameters of Dalbergia latifolia Roxb. Journal of Farm Sciences, 31 (1): 99–101.
Mahatara, D., Acharya, A.K., Dhakal, B.P., Sharma, D.K., Ulak, S. & Paudel, P. (2021). Maxent modelling for habitat suitability of vulnerable tree Dalbergia latifolia in Nepal. Silva Fennica, 55 (4): 10441. DOI: 10.14214/sf.10441.
Masilamani, P., Annadurai, K., Balamurugan, J. & Bhaskaran, M. (2012). Effect of different hydroxides on germination and seedling vigour of teak (Tectona grandis L. f.). Indian Journal of Forestry, 35 (2): 167–170. DOI: 10.54207/bsmps1000-2012-A2JYE3.
Nagaveni, H.C., Ananthapadmanabha, H.S. & Rai, S.N. (1989). Effect of different chemicals on germination of sandal seeds (Santalum album Linn.). My Forest, 25 (4): 311–313.
Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. & Simons, A. (2009). Agroforestry database: a tree reference and selection guide. Version 4.
Praciak, A., Pasiecznik, N., Sheil, D., van Heist, M., Sassen, M., Sousa Correia, C., Dixon, C., Fyson, G., Rushforth, K. & Teeling C (eds.) (2013). The CABI encyclopedia of forest trees. CABI, Oxfordshire, UK.
Rodríguez, S., Vargas, I., Hijuelo, A., Loumeto, F., Silva, J.J., Pérez, J., Arias, Q., Fonseca, Y., Gómez, Y., Baldoquìn, M. & Oliva, D. (2020). Analysis of the effect of scarification process on papaya (Carica papaya L.) Seeds Germination. IntechOpen. DOI: 10.5772/intechopen.88012.
Richards, D.E., King, K.E., Ait-Ali, T. & Harberd, N.P. (2001.) How gibberellin regulates plant growth and development: a molecular genetic analysis of gibberellin signaling. Annual Review of Plant Biology, 52 (1): 67–88. DOI: 10.1146/annurev.arplant.52.1.67.
Sehgal, H. (1983). Disease problem of Eucalyptus in India. Indian Forester, 109: 909–816.
Sukhadiya, M., Dholariya, C.A., Behera, L.K., Nayak, D., Patel, S.M. & Mehta, A.A. (2020). Dalbergia latifolia Roxb: Biography of an indigenous multipurpose tree species of India. MFP News, 1: 1–7.
Spyroglou, G. & Radoglou, K. (2017). Effect of pre-treatments on the germination of jasmin box (Phillyrea latifolia) seeds in Greece. Revista Bosque, 38(2): 347–355. DOI: 10.4067/S0717-92002017000200012.
Swamy, B.R., Himabindu, K. & Sita, G.L. (1992). In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.). Plant Cell Reports, 11: 126–131. DOI: 10.1007/BF00232164.
Tanaka-Oda, A., Kenzo, T. & Fukuda, K. (2009). Optimal germination condition by sulfuric acid pretreatment to improve seed germination of Sabina vulgaris Ant. Journal of Forest Research, 14: 251–256. DOI: 10.1007/s10310-009-0129-5.
Wang, N., Zhang, Z., Xu, W., Zhou, H. & Ning, R. (2022). Research progress on dormancy mechanism and germination technology of Kobresia seeds. Plants, 11 (23): 3192. DOI: 10.3390/plants11233192.
Verma, P., Soni, K.K., Verma, R.K. & Shirin, F. (2016). Seedling die-back of Dalbergia latifolia in Central India caused by Colletotrichum capsici-A new record. International Journal of Current Microbiological Applied Science, 5 (6): 350–356. DOI: 10.20546/ijcmas.2016.506.040.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Borneo Journal of Resource Science and Technology

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright Transfer Statement for Journal
1) In signing this statement, the author(s) grant UNIMAS Publisher an exclusive license to publish their original research papers. The author(s) also grant UNIMAS Publisher permission to reproduce, recreate, translate, extract or summarize, and to distribute and display in any forms, formats, and media. The author(s) can reuse their papers in their future printed work without first requiring permission from UNIMAS Publisher, provided that the author(s) acknowledge and reference publication in the Journal.
2) For open access articles, the author(s) agree that their articles published under UNIMAS Publisher are distributed under the terms of the CC-BY-NC-SA (Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License) which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original work of the author(s) is properly cited.
3) For subscription articles, the author(s) agree that UNIMAS Publisher holds copyright, or an exclusive license to publish. Readers or users may view, download, print, and copy the content, for academic purposes, subject to the following conditions of use: (a) any reuse of materials is subject to permission from UNIMAS Publisher; (b) archived materials may only be used for academic research; (c) archived materials may not be used for commercial purposes, which include but not limited to monetary compensation by means of sale, resale, license, transfer of copyright, loan, etc.; and (d) archived materials may not be re-published in any part, either in print or online.
4) The author(s) is/are responsible to ensure his or her or their submitted work is original and does not infringe any existing copyright, trademark, patent, statutory right, or propriety right of others. Corresponding author(s) has (have) obtained permission from all co-authors prior to submission to the journal. Upon submission of the manuscript, the author(s) agree that no similar work has been or will be submitted or published elsewhere in any language. If submitted manuscript includes materials from others, the authors have obtained the permission from the copyright owners.
5) In signing this statement, the author(s) declare(s) that the researches in which they have conducted are in compliance with the current laws of the respective country and UNIMAS Journal Publication Ethics Policy. Any experimentation or research involving human or the use of animal samples must obtain approval from Human or Animal Ethics Committee in their respective institutions. The author(s) agree and understand that UNIMAS Publisher is not responsible for any compensational claims or failure caused by the author(s) in fulfilling the above-mentioned requirements. The author(s) must accept the responsibility for releasing their materials upon request by Chief Editor or UNIMAS Publisher.
6) The author(s) should have participated sufficiently in the work and ensured the appropriateness of the content of the article. The author(s) should also agree that he or she has no commercial attachments (e.g. patent or license arrangement, equity interest, consultancies, etc.) that might pose any conflict of interest with the submitted manuscript. The author(s) also agree to make any relevant materials and data available upon request by the editor or UNIMAS Publisher.