Variations and Hybridization Compatibility of Single Basidiospore Isolates of Pleurotus sajor-caju (Fr.) Sings
Abstract
Pleurotus sajor-caju (Fr.) Sings, a mushroom of the family Pleurotaceae, is gaining popularity due to its high nutrient content and capability of growing on various agricultural wastes. There is a need to breed new strain of P. sajor-caju to meet the rising demands of the increasing human population. Strain improvement is achievable through selection and hybridization. Unfortunately, there is limited information regarding the genetic variations of P. sajor caju in Malaysia. Therefore, this study is of interest to document the morphological variations of single basidiospore isolates and to generate hybrids. A total of 200 single basidiospore isolates (SB) obtained from a commercialized strain of P. sajor-caju were obtained from local supermarket in Kuching, Sarawak, and cultured individually on potato dextrose agar. These 200 SBs were characterized morphologically and divided into three main groups based on colony morphology i.e. scattered, rough and smooth. Variations can still be observed in each main group. From each main group, SBs representing the variations were further categorized based on their colony diameter growth after 7 days of post inoculation (CD-7dpi), i.e. slow growing CD-7dpi (SGCD-7), medium fast growing CD-7dpi (MFGCD-7) and fast growing CD-7dpi (FGCD-7). Ten FGCD-7 and ten SGCD-7 isolates were selected for hybridization. The selected SBs were hybridized in all possible pairings without repetition. Sixteen hybridized isolates were recognized and characterized based on CD-7dpi. For all FGCD-7 pairings, SGCD-7 pairings, and between FGCD-7 and SGCD-7 pairings, hybridized isolates had higher CD-7dpi than at least one of its parents were identified. The new hybridized isolates are interesting materials for future study.
References
Esser, K. & Blaich, R. (1994). Heterogenetics incompatibility in fungi. In Wessels, J.G.H. & Meinhardt, F. (Eds.), The Mycota I: Growth, differentiation and sexuality. Springer, Berlin Heidelberg New York. Pp 211-232.
Gharehaghaji, A.N., Goltapeh, E.M., Masiha, S., & Gordan, H.R. (2007). Hybrid production of oyster mushroom Pleurotus ostreatus (Jacq: Fries) Kummer. Pakistan Journal of Biological Sciences, 10(14): 2334-2340.
Gupta, B., Reddy, B.P.N., & Kotasthane, A.S. (2011). Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement. World Journal of Microbiology and Biotechnology, 27: 1-9.
Ikeda, K., Nakamura, H., & Matsumoto, N. (2002). Mycelial incompatibility operative in pairings between single basidiospore isolates of Helicobasidium mompa. Mycological Research, 107: 847-853.
https://doi.org/10.1017/S0953756203008049
Imran, M.M., Raja, M.M., Basith, M.A., & Asarudin, A. (2011). Determination of total phenol, flavanoid and antioxidant activity of edible mushrooms Pleurotus florida and Pleurotus eous. International Food Research Journal, 18: 574-577.
Kothe, E. (2001). Mating-type genes for basidiomycete strain improvement in mushroom farming. Applied Microbiology and Biotechnology, 56: 602-612.
Kronstad, J.W. & Staben, C. (1997). Mating type in filamentous fungi. Annual Review of Genetics, 31: 245-276.
Micali, O.C. & Smith, M.L. (2005). Biological concepts of vegetative self and nonself recognition in fungi. In Xu, J. (Ed). Evolutionary genetics of fungi. UK: Horizon Scientific Press. Pp 63-85.
Pala, S.A., Wani, A.H., & Mir, R.A. (2012). Yield performance of Pleurotus sajor-caju on different agro-based wastes. Annals of Biological Research, 3(4): 1938-1941.
Rosli, W.I. & Solihah, M.A. (2012). Effect on the addition of Pleurotus sajor-caju (PSC) on the physical and sensorial properties of beef patty. International Food Research Journal, 19(3): 993-999.
Sadler, M. (2003). Nutritional properties of edible fungi. British Nutrition Foundation Bulletin, 28: 305-308.
https://doi.org/10.1046/j.1467-3010.2003.00354.x
Schneider, I., Kressel, G., Meyer, A., Krings, U., Berger, R.G., & Hahn, A. (2011). Lipid lowering effects of oyster mushroom (Pleurotus ostreatus) in humans. Journal of Functional Foods, 3(1): 17-24.
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