A PRELIMINARY STUDY ON ANTIMICROBIAL ACTIVITY OF Imperata cylindrica

  • Ahmad Fahmi Harun Ismail
  • Othman Abd Samah
  • Abubakar Sule
Keywords: Antimicrobial activity, disc diffusion method, clinical isolates, inhibition zone.

Abstract

Antimicrobial properties of the methanol, chloroform or polybutylene succinate (PBS) extracts of leaves and rhizome from Imperata cylindrica were investigated against five clinical isolates of bacteria, namely Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. In disc diffusion test the three types of extracts showed varied levels of antimicrobial activity against the tested isolates, with inhibition zones ranging from 6.33 ± 0.58 to 11.67 ± 8.14 mm diameters. The highest activity was exerted by the methanol leaves extract on P. aeruginosa (11.67 ± 8.14mm) at 50 mg/ml and the least activity was exerted by both the leaves and rhizome extracts of the plant at 1mg/ml on B. subtilis, P. aeruginosa, S. aureus and E.coli (6.33 ± 0.58mm) respectively. A minimum inhibitory concentration (MIC) value of 25mg/ml was obtained for the methanol, chloroform, PBS leaves or rhizome extracts on B. subtilis, P. aeruginosa, S. aureus and E. coli

References

Bagyalakshmi, B., Sridhar, D., Ponmurugan, P. (2009). Antimicrobial activity of important Indian medicinal plants against pyogenic infection. Journal of Phytology, 1(6): 391-396.

Chunlaratthanaphorn, S., Lertprasertsuke, N., Srisawat, U., Thuppia, A., Ngamjariyawat, A., Suwanlikhid, N., Jaijoy, K. (2007). Acute and subchronic toxicity study of the water extract from root of Imperata cylindrica (Linn.) Raeusch. in rats. Journal of Vacuum Science and Technology, 29(Suppl. 1): 141-155.

Eloff, J.N. (1998). A sensitive and quick method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica, 64: 711-713.

https://doi.org/10.1055/s-2006-957563

Harborne, J.B. (1998). Phytochemical methods: A guide to modern techniques on plant analysis, 3rd edition. United Kingdom: Kluwer Academic Publishers, pp. 78-84.

MacDonald, G.E. (2004). Cogongrass (Imperata cylindrica)-Biology, Ecology, and Management. Critical Reviews in Plant Science, 23: 367-380.

https://doi.org/10.1080/07352680490505114

National Committee for Clinical Laboratory Standards (NCCLS). (2006). Perfomance Standards for Antimicrobial Susceptibility Tests; Approved Standards- 9th edition. Vol 28 No 8.

Osbourn., A.E. (1996). Preformed antimicrobial compounds and plant defence against fungal attack. The Plant Cell, 8: 1821-1831.

https://doi.org/10.1105/tpc.8.10.1821

Published
2016-06-29
How to Cite
Harun Ismail, A. F., Samah, O. A., & Sule, A. (2016). A PRELIMINARY STUDY ON ANTIMICROBIAL ACTIVITY OF Imperata cylindrica. Borneo Journal of Resource Science and Technology, 1(1), 63-66. https://doi.org/10.33736/bjrst.266.2011
Section
General